Job ID = 10166258 SRX = SRX9008799 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 61710374 spots for SRR12518271/SRR12518271.sra Written 61710374 spots for SRR12518271/SRR12518271.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166669 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:39 61710374 reads; of these: 61710374 (100.00%) were unpaired; of these: 32805580 (53.16%) aligned 0 times 23341050 (37.82%) aligned exactly 1 time 5563744 (9.02%) aligned >1 times 46.84% overall alignment rate Time searching: 00:11:39 Overall time: 00:11:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6705397 / 28904794 = 0.2320 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:04:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:04:48: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:04:48: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:04:53: 1000000 INFO @ Thu, 08 Oct 2020 21:04:57: 2000000 INFO @ Thu, 08 Oct 2020 21:05:02: 3000000 INFO @ Thu, 08 Oct 2020 21:05:06: 4000000 INFO @ Thu, 08 Oct 2020 21:05:11: 5000000 INFO @ Thu, 08 Oct 2020 21:05:15: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:05:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:05:18: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:05:18: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:05:20: 7000000 INFO @ Thu, 08 Oct 2020 21:05:23: 1000000 INFO @ Thu, 08 Oct 2020 21:05:24: 8000000 INFO @ Thu, 08 Oct 2020 21:05:28: 2000000 INFO @ Thu, 08 Oct 2020 21:05:29: 9000000 INFO @ Thu, 08 Oct 2020 21:05:32: 3000000 INFO @ Thu, 08 Oct 2020 21:05:34: 10000000 INFO @ Thu, 08 Oct 2020 21:05:37: 4000000 INFO @ Thu, 08 Oct 2020 21:05:38: 11000000 INFO @ Thu, 08 Oct 2020 21:05:41: 5000000 INFO @ Thu, 08 Oct 2020 21:05:43: 12000000 INFO @ Thu, 08 Oct 2020 21:05:46: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:05:47: 13000000 INFO @ Thu, 08 Oct 2020 21:05:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:05:48: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:05:48: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:05:51: 7000000 INFO @ Thu, 08 Oct 2020 21:05:52: 14000000 INFO @ Thu, 08 Oct 2020 21:05:53: 1000000 INFO @ Thu, 08 Oct 2020 21:05:56: 8000000 INFO @ Thu, 08 Oct 2020 21:05:56: 15000000 INFO @ Thu, 08 Oct 2020 21:05:58: 2000000 INFO @ Thu, 08 Oct 2020 21:06:01: 16000000 INFO @ Thu, 08 Oct 2020 21:06:01: 9000000 INFO @ Thu, 08 Oct 2020 21:06:02: 3000000 INFO @ Thu, 08 Oct 2020 21:06:06: 17000000 INFO @ Thu, 08 Oct 2020 21:06:06: 10000000 INFO @ Thu, 08 Oct 2020 21:06:07: 4000000 INFO @ Thu, 08 Oct 2020 21:06:11: 18000000 INFO @ Thu, 08 Oct 2020 21:06:11: 11000000 INFO @ Thu, 08 Oct 2020 21:06:12: 5000000 INFO @ Thu, 08 Oct 2020 21:06:15: 19000000 INFO @ Thu, 08 Oct 2020 21:06:16: 12000000 INFO @ Thu, 08 Oct 2020 21:06:16: 6000000 INFO @ Thu, 08 Oct 2020 21:06:20: 20000000 INFO @ Thu, 08 Oct 2020 21:06:21: 13000000 INFO @ Thu, 08 Oct 2020 21:06:21: 7000000 INFO @ Thu, 08 Oct 2020 21:06:25: 21000000 INFO @ Thu, 08 Oct 2020 21:06:26: 8000000 INFO @ Thu, 08 Oct 2020 21:06:26: 14000000 INFO @ Thu, 08 Oct 2020 21:06:29: 22000000 INFO @ Thu, 08 Oct 2020 21:06:31: 9000000 INFO @ Thu, 08 Oct 2020 21:06:31: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 21:06:31: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 21:06:31: #1 total tags in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:06:31: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:06:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:06:31: #1 tags after filtering in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:06:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:06:31: #1 finished! INFO @ Thu, 08 Oct 2020 21:06:31: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:06:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:06:31: 15000000 INFO @ Thu, 08 Oct 2020 21:06:32: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:06:32: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:06:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:06:35: 10000000 INFO @ Thu, 08 Oct 2020 21:06:36: 16000000 INFO @ Thu, 08 Oct 2020 21:06:40: 11000000 INFO @ Thu, 08 Oct 2020 21:06:40: 17000000 INFO @ Thu, 08 Oct 2020 21:06:44: 12000000 INFO @ Thu, 08 Oct 2020 21:06:45: 18000000 INFO @ Thu, 08 Oct 2020 21:06:49: 13000000 INFO @ Thu, 08 Oct 2020 21:06:50: 19000000 INFO @ Thu, 08 Oct 2020 21:06:54: 14000000 INFO @ Thu, 08 Oct 2020 21:06:54: 20000000 INFO @ Thu, 08 Oct 2020 21:06:58: 15000000 INFO @ Thu, 08 Oct 2020 21:06:59: 21000000 INFO @ Thu, 08 Oct 2020 21:07:03: 16000000 INFO @ Thu, 08 Oct 2020 21:07:04: 22000000 INFO @ Thu, 08 Oct 2020 21:07:05: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 21:07:05: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 21:07:05: #1 total tags in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:07:05: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:07:05: #1 tags after filtering in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:07:05: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:07:05: #1 finished! INFO @ Thu, 08 Oct 2020 21:07:05: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:07:05: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:07:07: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:07:07: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:07:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:07:07: 17000000 INFO @ Thu, 08 Oct 2020 21:07:12: 18000000 INFO @ Thu, 08 Oct 2020 21:07:17: 19000000 INFO @ Thu, 08 Oct 2020 21:07:21: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:07:26: 21000000 INFO @ Thu, 08 Oct 2020 21:07:30: 22000000 INFO @ Thu, 08 Oct 2020 21:07:32: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 21:07:32: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 21:07:32: #1 total tags in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:07:32: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:07:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:07:32: #1 tags after filtering in treatment: 22199397 INFO @ Thu, 08 Oct 2020 21:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:07:32: #1 finished! INFO @ Thu, 08 Oct 2020 21:07:32: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:07:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:07:33: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:07:33: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:07:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。