Job ID = 14172079 SRX = SRX9008799 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 61710374 spots for SRR12518271/SRR12518271.sra Written 61710374 spots for SRR12518271/SRR12518271.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172552 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:20 61710374 reads; of these: 61710374 (100.00%) were unpaired; of these: 32805580 (53.16%) aligned 0 times 23341050 (37.82%) aligned exactly 1 time 5563744 (9.02%) aligned >1 times 46.84% overall alignment rate Time searching: 00:13:20 Overall time: 00:13:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6705397 / 28904794 = 0.2320 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:25:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:25:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:25:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:25:42: 1000000 INFO @ Sat, 11 Dec 2021 14:25:48: 2000000 INFO @ Sat, 11 Dec 2021 14:25:54: 3000000 INFO @ Sat, 11 Dec 2021 14:26:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:26:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:26:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:26:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:26:08: 5000000 INFO @ Sat, 11 Dec 2021 14:26:12: 1000000 INFO @ Sat, 11 Dec 2021 14:26:15: 6000000 INFO @ Sat, 11 Dec 2021 14:26:18: 2000000 INFO @ Sat, 11 Dec 2021 14:26:21: 7000000 INFO @ Sat, 11 Dec 2021 14:26:25: 3000000 INFO @ Sat, 11 Dec 2021 14:26:28: 8000000 INFO @ Sat, 11 Dec 2021 14:26:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:26:35: 9000000 INFO @ Sat, 11 Dec 2021 14:26:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:26:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:26:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:26:38: 5000000 INFO @ Sat, 11 Dec 2021 14:26:42: 10000000 INFO @ Sat, 11 Dec 2021 14:26:42: 1000000 INFO @ Sat, 11 Dec 2021 14:26:45: 6000000 INFO @ Sat, 11 Dec 2021 14:26:49: 11000000 INFO @ Sat, 11 Dec 2021 14:26:49: 2000000 INFO @ Sat, 11 Dec 2021 14:26:52: 7000000 INFO @ Sat, 11 Dec 2021 14:26:56: 12000000 INFO @ Sat, 11 Dec 2021 14:26:57: 3000000 INFO @ Sat, 11 Dec 2021 14:26:59: 8000000 INFO @ Sat, 11 Dec 2021 14:27:03: 13000000 INFO @ Sat, 11 Dec 2021 14:27:04: 4000000 INFO @ Sat, 11 Dec 2021 14:27:06: 9000000 INFO @ Sat, 11 Dec 2021 14:27:09: 14000000 INFO @ Sat, 11 Dec 2021 14:27:11: 5000000 INFO @ Sat, 11 Dec 2021 14:27:13: 10000000 INFO @ Sat, 11 Dec 2021 14:27:16: 15000000 INFO @ Sat, 11 Dec 2021 14:27:18: 6000000 INFO @ Sat, 11 Dec 2021 14:27:19: 11000000 INFO @ Sat, 11 Dec 2021 14:27:23: 16000000 INFO @ Sat, 11 Dec 2021 14:27:25: 7000000 INFO @ Sat, 11 Dec 2021 14:27:26: 12000000 INFO @ Sat, 11 Dec 2021 14:27:30: 17000000 INFO @ Sat, 11 Dec 2021 14:27:33: 8000000 INFO @ Sat, 11 Dec 2021 14:27:34: 13000000 INFO @ Sat, 11 Dec 2021 14:27:37: 18000000 INFO @ Sat, 11 Dec 2021 14:27:40: 9000000 INFO @ Sat, 11 Dec 2021 14:27:41: 14000000 INFO @ Sat, 11 Dec 2021 14:27:44: 19000000 INFO @ Sat, 11 Dec 2021 14:27:47: 10000000 INFO @ Sat, 11 Dec 2021 14:27:48: 15000000 INFO @ Sat, 11 Dec 2021 14:27:51: 20000000 INFO @ Sat, 11 Dec 2021 14:27:53: 11000000 INFO @ Sat, 11 Dec 2021 14:27:55: 16000000 INFO @ Sat, 11 Dec 2021 14:27:58: 21000000 INFO @ Sat, 11 Dec 2021 14:28:00: 12000000 INFO @ Sat, 11 Dec 2021 14:28:01: 17000000 INFO @ Sat, 11 Dec 2021 14:28:04: 22000000 INFO @ Sat, 11 Dec 2021 14:28:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:28:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:28:06: #1 total tags in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:28:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:28:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:28:06: #1 tags after filtering in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:28:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:28:06: #1 finished! INFO @ Sat, 11 Dec 2021 14:28:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:28:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:28:07: 13000000 INFO @ Sat, 11 Dec 2021 14:28:08: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 14:28:08: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:28:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:28:08: 18000000 INFO @ Sat, 11 Dec 2021 14:28:14: 14000000 INFO @ Sat, 11 Dec 2021 14:28:15: 19000000 INFO @ Sat, 11 Dec 2021 14:28:20: 15000000 INFO @ Sat, 11 Dec 2021 14:28:22: 20000000 INFO @ Sat, 11 Dec 2021 14:28:27: 16000000 INFO @ Sat, 11 Dec 2021 14:28:28: 21000000 INFO @ Sat, 11 Dec 2021 14:28:34: 17000000 INFO @ Sat, 11 Dec 2021 14:28:35: 22000000 INFO @ Sat, 11 Dec 2021 14:28:37: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:28:37: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:28:37: #1 total tags in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:28:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:28:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:28:37: #1 tags after filtering in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:28:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:28:37: #1 finished! INFO @ Sat, 11 Dec 2021 14:28:37: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:28:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:28:39: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 14:28:39: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:28:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:28:40: 18000000 INFO @ Sat, 11 Dec 2021 14:28:47: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:28:53: 20000000 INFO @ Sat, 11 Dec 2021 14:29:00: 21000000 INFO @ Sat, 11 Dec 2021 14:29:06: 22000000 INFO @ Sat, 11 Dec 2021 14:29:08: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:29:08: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:29:08: #1 total tags in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:29:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:29:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:29:08: #1 tags after filtering in treatment: 22199397 INFO @ Sat, 11 Dec 2021 14:29:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:29:08: #1 finished! INFO @ Sat, 11 Dec 2021 14:29:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:29:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:29:10: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 14:29:10: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:29:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008799/SRX9008799.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。