Job ID = 14171006 SRX = SRX9005702 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 762254 READS because READLEN < 1 Read 9699779 spots for SRR12515103/SRR12515103.sra Written 9699779 spots for SRR12515103/SRR12515103.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171525 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] 8 unmatched pairs [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2539662 / 7818206 = 0.3248 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:39:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:39:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:39:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:39:54: 1000000 INFO @ Sat, 11 Dec 2021 09:40:00: 2000000 INFO @ Sat, 11 Dec 2021 09:40:06: 3000000 INFO @ Sat, 11 Dec 2021 09:40:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:40:18: 5000000 INFO @ Sat, 11 Dec 2021 09:40:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:40:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:40:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:40:25: 6000000 INFO @ Sat, 11 Dec 2021 09:40:26: 1000000 INFO @ Sat, 11 Dec 2021 09:40:31: 7000000 INFO @ Sat, 11 Dec 2021 09:40:33: 2000000 INFO @ Sat, 11 Dec 2021 09:40:38: 8000000 INFO @ Sat, 11 Dec 2021 09:40:41: 3000000 INFO @ Sat, 11 Dec 2021 09:40:44: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:40:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:40:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:40:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:40:49: 4000000 INFO @ Sat, 11 Dec 2021 09:40:51: 10000000 INFO @ Sat, 11 Dec 2021 09:40:55: 1000000 INFO @ Sat, 11 Dec 2021 09:40:56: 5000000 INFO @ Sat, 11 Dec 2021 09:40:58: 11000000 INFO @ Sat, 11 Dec 2021 09:41:01: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 09:41:01: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 09:41:01: #1 total tags in treatment: 5171093 INFO @ Sat, 11 Dec 2021 09:41:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:41:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:41:01: #1 tags after filtering in treatment: 4389343 INFO @ Sat, 11 Dec 2021 09:41:01: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 11 Dec 2021 09:41:01: #1 finished! INFO @ Sat, 11 Dec 2021 09:41:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:41:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:41:01: 2000000 INFO @ Sat, 11 Dec 2021 09:41:02: #2 number of paired peaks: 5375 INFO @ Sat, 11 Dec 2021 09:41:02: start model_add_line... INFO @ Sat, 11 Dec 2021 09:41:02: start X-correlation... INFO @ Sat, 11 Dec 2021 09:41:02: end of X-cor INFO @ Sat, 11 Dec 2021 09:41:02: #2 finished! INFO @ Sat, 11 Dec 2021 09:41:02: #2 predicted fragment length is 222 bps INFO @ Sat, 11 Dec 2021 09:41:02: #2 alternative fragment length(s) may be 222 bps INFO @ Sat, 11 Dec 2021 09:41:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05_model.r WARNING @ Sat, 11 Dec 2021 09:41:02: #2 Since the d (222) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:41:02: #2 You may need to consider one of the other alternative d(s): 222 WARNING @ Sat, 11 Dec 2021 09:41:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:41:02: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:41:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:41:04: 6000000 INFO @ Sat, 11 Dec 2021 09:41:08: 3000000 INFO @ Sat, 11 Dec 2021 09:41:12: 7000000 INFO @ Sat, 11 Dec 2021 09:41:14: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:41:15: 4000000 INFO @ Sat, 11 Dec 2021 09:41:19: 8000000 INFO @ Sat, 11 Dec 2021 09:41:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:41:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:41:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.05_summits.bed INFO @ Sat, 11 Dec 2021 09:41:19: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6717 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:41:21: 5000000 INFO @ Sat, 11 Dec 2021 09:41:27: 9000000 INFO @ Sat, 11 Dec 2021 09:41:28: 6000000 INFO @ Sat, 11 Dec 2021 09:41:34: 7000000 INFO @ Sat, 11 Dec 2021 09:41:35: 10000000 INFO @ Sat, 11 Dec 2021 09:41:41: 8000000 INFO @ Sat, 11 Dec 2021 09:41:42: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:41:46: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 09:41:46: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 09:41:46: #1 total tags in treatment: 5171093 INFO @ Sat, 11 Dec 2021 09:41:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:41:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:41:46: #1 tags after filtering in treatment: 4389343 INFO @ Sat, 11 Dec 2021 09:41:46: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 11 Dec 2021 09:41:46: #1 finished! INFO @ Sat, 11 Dec 2021 09:41:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:41:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:41:47: #2 number of paired peaks: 5375 INFO @ Sat, 11 Dec 2021 09:41:47: start model_add_line... INFO @ Sat, 11 Dec 2021 09:41:47: start X-correlation... INFO @ Sat, 11 Dec 2021 09:41:47: end of X-cor INFO @ Sat, 11 Dec 2021 09:41:47: #2 finished! INFO @ Sat, 11 Dec 2021 09:41:47: #2 predicted fragment length is 222 bps INFO @ Sat, 11 Dec 2021 09:41:47: #2 alternative fragment length(s) may be 222 bps INFO @ Sat, 11 Dec 2021 09:41:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10_model.r WARNING @ Sat, 11 Dec 2021 09:41:47: #2 Since the d (222) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:41:47: #2 You may need to consider one of the other alternative d(s): 222 WARNING @ Sat, 11 Dec 2021 09:41:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:41:47: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:41:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:41:47: 9000000 INFO @ Sat, 11 Dec 2021 09:41:54: 10000000 INFO @ Sat, 11 Dec 2021 09:41:59: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:42:00: 11000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:42:03: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 09:42:03: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 09:42:03: #1 total tags in treatment: 5171093 INFO @ Sat, 11 Dec 2021 09:42:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:42:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:42:04: #1 tags after filtering in treatment: 4389343 INFO @ Sat, 11 Dec 2021 09:42:04: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 11 Dec 2021 09:42:04: #1 finished! INFO @ Sat, 11 Dec 2021 09:42:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:42:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:42:04: #2 number of paired peaks: 5375 INFO @ Sat, 11 Dec 2021 09:42:04: start model_add_line... INFO @ Sat, 11 Dec 2021 09:42:04: start X-correlation... INFO @ Sat, 11 Dec 2021 09:42:04: end of X-cor INFO @ Sat, 11 Dec 2021 09:42:04: #2 finished! INFO @ Sat, 11 Dec 2021 09:42:04: #2 predicted fragment length is 222 bps INFO @ Sat, 11 Dec 2021 09:42:04: #2 alternative fragment length(s) may be 222 bps INFO @ Sat, 11 Dec 2021 09:42:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20_model.r WARNING @ Sat, 11 Dec 2021 09:42:04: #2 Since the d (222) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:42:04: #2 You may need to consider one of the other alternative d(s): 222 WARNING @ Sat, 11 Dec 2021 09:42:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:42:04: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:42:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:42:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:42:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:42:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.10_summits.bed INFO @ Sat, 11 Dec 2021 09:42:04: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4916 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:42:16: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:42:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:42:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:42:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005702/SRX9005702.20_summits.bed INFO @ Sat, 11 Dec 2021 09:42:21: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3284 records, 4 fields): 5 millis CompletedMACS2peakCalling