Job ID = 14171262 SRX = SRX9005654 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 1034865 READS because READLEN < 1 Read 5837248 spots for SRR12515055/SRR12515055.sra Written 5837248 spots for SRR12515055/SRR12515055.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171742 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] 3 unmatched pairs [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] 2 unmatched pairs [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] 16 unmatched pairs [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 531193 / 4382325 = 0.1212 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:10:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:10:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:10:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:10:35: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:10:50: 2000000 INFO @ Sat, 11 Dec 2021 11:10:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:10:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:10:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:11:05: 3000000 INFO @ Sat, 11 Dec 2021 11:11:06: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:11:19: 4000000 INFO @ Sat, 11 Dec 2021 11:11:20: 2000000 INFO @ Sat, 11 Dec 2021 11:11:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:11:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:11:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:11:33: 5000000 INFO @ Sat, 11 Dec 2021 11:11:34: 3000000 INFO @ Sat, 11 Dec 2021 11:11:36: 1000000 INFO @ Sat, 11 Dec 2021 11:11:48: 4000000 INFO @ Sat, 11 Dec 2021 11:11:48: 6000000 INFO @ Sat, 11 Dec 2021 11:11:52: 2000000 INFO @ Sat, 11 Dec 2021 11:12:02: 5000000 INFO @ Sat, 11 Dec 2021 11:12:04: 7000000 INFO @ Sat, 11 Dec 2021 11:12:07: 3000000 INFO @ Sat, 11 Dec 2021 11:12:16: 6000000 INFO @ Sat, 11 Dec 2021 11:12:19: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:12:22: 4000000 INFO @ Sat, 11 Dec 2021 11:12:29: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 11:12:29: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 11:12:29: #1 total tags in treatment: 3760557 INFO @ Sat, 11 Dec 2021 11:12:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:12:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:12:29: #1 tags after filtering in treatment: 3027254 INFO @ Sat, 11 Dec 2021 11:12:29: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 11 Dec 2021 11:12:29: #1 finished! INFO @ Sat, 11 Dec 2021 11:12:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:12:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:12:30: #2 number of paired peaks: 7298 INFO @ Sat, 11 Dec 2021 11:12:30: start model_add_line... INFO @ Sat, 11 Dec 2021 11:12:30: start X-correlation... INFO @ Sat, 11 Dec 2021 11:12:30: end of X-cor INFO @ Sat, 11 Dec 2021 11:12:30: #2 finished! INFO @ Sat, 11 Dec 2021 11:12:30: #2 predicted fragment length is 219 bps INFO @ Sat, 11 Dec 2021 11:12:30: #2 alternative fragment length(s) may be 219 bps INFO @ Sat, 11 Dec 2021 11:12:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05_model.r WARNING @ Sat, 11 Dec 2021 11:12:30: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 11:12:30: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Sat, 11 Dec 2021 11:12:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 11:12:30: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:12:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:12:30: 7000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 11:12:37: 5000000 INFO @ Sat, 11 Dec 2021 11:12:41: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:12:44: 8000000 INFO @ Sat, 11 Dec 2021 11:12:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05_peaks.xls INFO @ Sat, 11 Dec 2021 11:12:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:12:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.05_summits.bed INFO @ Sat, 11 Dec 2021 11:12:46: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (8393 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:12:52: 6000000 INFO @ Sat, 11 Dec 2021 11:12:53: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 11:12:53: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 11:12:53: #1 total tags in treatment: 3760557 INFO @ Sat, 11 Dec 2021 11:12:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:12:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:12:53: #1 tags after filtering in treatment: 3027254 INFO @ Sat, 11 Dec 2021 11:12:53: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 11 Dec 2021 11:12:53: #1 finished! INFO @ Sat, 11 Dec 2021 11:12:53: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:12:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:12:54: #2 number of paired peaks: 7298 INFO @ Sat, 11 Dec 2021 11:12:54: start model_add_line... INFO @ Sat, 11 Dec 2021 11:12:54: start X-correlation... INFO @ Sat, 11 Dec 2021 11:12:54: end of X-cor INFO @ Sat, 11 Dec 2021 11:12:54: #2 finished! INFO @ Sat, 11 Dec 2021 11:12:54: #2 predicted fragment length is 219 bps INFO @ Sat, 11 Dec 2021 11:12:54: #2 alternative fragment length(s) may be 219 bps INFO @ Sat, 11 Dec 2021 11:12:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10_model.r WARNING @ Sat, 11 Dec 2021 11:12:54: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 11:12:54: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Sat, 11 Dec 2021 11:12:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 11:12:54: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:12:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:13:05: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:13:07: 7000000 INFO @ Sat, 11 Dec 2021 11:13:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10_peaks.xls INFO @ Sat, 11 Dec 2021 11:13:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:13:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.10_summits.bed INFO @ Sat, 11 Dec 2021 11:13:10: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6544 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:13:21: 8000000 INFO @ Sat, 11 Dec 2021 11:13:30: #1 tag size is determined as 125 bps INFO @ Sat, 11 Dec 2021 11:13:30: #1 tag size = 125 INFO @ Sat, 11 Dec 2021 11:13:30: #1 total tags in treatment: 3760557 INFO @ Sat, 11 Dec 2021 11:13:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:13:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:13:30: #1 tags after filtering in treatment: 3027254 INFO @ Sat, 11 Dec 2021 11:13:30: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 11 Dec 2021 11:13:30: #1 finished! INFO @ Sat, 11 Dec 2021 11:13:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:13:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:13:31: #2 number of paired peaks: 7298 INFO @ Sat, 11 Dec 2021 11:13:31: start model_add_line... INFO @ Sat, 11 Dec 2021 11:13:31: start X-correlation... INFO @ Sat, 11 Dec 2021 11:13:31: end of X-cor INFO @ Sat, 11 Dec 2021 11:13:31: #2 finished! INFO @ Sat, 11 Dec 2021 11:13:31: #2 predicted fragment length is 219 bps INFO @ Sat, 11 Dec 2021 11:13:31: #2 alternative fragment length(s) may be 219 bps INFO @ Sat, 11 Dec 2021 11:13:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20_model.r WARNING @ Sat, 11 Dec 2021 11:13:31: #2 Since the d (219) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 11:13:31: #2 You may need to consider one of the other alternative d(s): 219 WARNING @ Sat, 11 Dec 2021 11:13:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 11:13:31: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:13:31: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:13:42: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:13:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20_peaks.xls INFO @ Sat, 11 Dec 2021 11:13:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:13:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005654/SRX9005654.20_summits.bed INFO @ Sat, 11 Dec 2021 11:13:46: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4632 records, 4 fields): 8 millis CompletedMACS2peakCalling