Job ID = 16436799 SRX = SRX8948322 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 245855 READS because READLEN < 1 Read 20577930 spots for SRR12453879/SRR12453879.sra Written 20577930 spots for SRR12453879/SRR12453879.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436973 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] 94 unmatched pairs [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] 4 unmatched pairs [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3796256 / 19958128 = 0.1902 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:12:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:12:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:12:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:12:33: 1000000 INFO @ Tue, 02 Aug 2022 12:12:38: 2000000 INFO @ Tue, 02 Aug 2022 12:12:43: 3000000 INFO @ Tue, 02 Aug 2022 12:12:47: 4000000 INFO @ Tue, 02 Aug 2022 12:12:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:12:57: 6000000 INFO @ Tue, 02 Aug 2022 12:12:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:12:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:12:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:13:02: 7000000 INFO @ Tue, 02 Aug 2022 12:13:03: 1000000 INFO @ Tue, 02 Aug 2022 12:13:08: 8000000 INFO @ Tue, 02 Aug 2022 12:13:09: 2000000 INFO @ Tue, 02 Aug 2022 12:13:13: 9000000 INFO @ Tue, 02 Aug 2022 12:13:14: 3000000 INFO @ Tue, 02 Aug 2022 12:13:19: 10000000 INFO @ Tue, 02 Aug 2022 12:13:19: 4000000 INFO @ Tue, 02 Aug 2022 12:13:24: 5000000 INFO @ Tue, 02 Aug 2022 12:13:24: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:13:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:13:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:13:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:13:29: 6000000 INFO @ Tue, 02 Aug 2022 12:13:29: 12000000 INFO @ Tue, 02 Aug 2022 12:13:33: 1000000 INFO @ Tue, 02 Aug 2022 12:13:34: 7000000 INFO @ Tue, 02 Aug 2022 12:13:34: 13000000 INFO @ Tue, 02 Aug 2022 12:13:39: 2000000 INFO @ Tue, 02 Aug 2022 12:13:39: 14000000 INFO @ Tue, 02 Aug 2022 12:13:39: 8000000 INFO @ Tue, 02 Aug 2022 12:13:44: 15000000 INFO @ Tue, 02 Aug 2022 12:13:44: 3000000 INFO @ Tue, 02 Aug 2022 12:13:44: 9000000 INFO @ Tue, 02 Aug 2022 12:13:49: 16000000 INFO @ Tue, 02 Aug 2022 12:13:49: 10000000 INFO @ Tue, 02 Aug 2022 12:13:49: 4000000 INFO @ Tue, 02 Aug 2022 12:13:54: 17000000 INFO @ Tue, 02 Aug 2022 12:13:54: 11000000 INFO @ Tue, 02 Aug 2022 12:13:55: 5000000 INFO @ Tue, 02 Aug 2022 12:13:59: 18000000 INFO @ Tue, 02 Aug 2022 12:14:00: 12000000 INFO @ Tue, 02 Aug 2022 12:14:00: 6000000 INFO @ Tue, 02 Aug 2022 12:14:04: 19000000 INFO @ Tue, 02 Aug 2022 12:14:05: 13000000 INFO @ Tue, 02 Aug 2022 12:14:05: 7000000 INFO @ Tue, 02 Aug 2022 12:14:09: 20000000 INFO @ Tue, 02 Aug 2022 12:14:11: 14000000 INFO @ Tue, 02 Aug 2022 12:14:11: 8000000 INFO @ Tue, 02 Aug 2022 12:14:14: 21000000 INFO @ Tue, 02 Aug 2022 12:14:16: 15000000 INFO @ Tue, 02 Aug 2022 12:14:16: 9000000 INFO @ Tue, 02 Aug 2022 12:14:19: 22000000 INFO @ Tue, 02 Aug 2022 12:14:21: 16000000 INFO @ Tue, 02 Aug 2022 12:14:21: 10000000 INFO @ Tue, 02 Aug 2022 12:14:24: 23000000 INFO @ Tue, 02 Aug 2022 12:14:27: 11000000 INFO @ Tue, 02 Aug 2022 12:14:27: 17000000 INFO @ Tue, 02 Aug 2022 12:14:29: 24000000 INFO @ Tue, 02 Aug 2022 12:14:32: 12000000 INFO @ Tue, 02 Aug 2022 12:14:32: 18000000 INFO @ Tue, 02 Aug 2022 12:14:34: 25000000 INFO @ Tue, 02 Aug 2022 12:14:37: 13000000 INFO @ Tue, 02 Aug 2022 12:14:37: 19000000 INFO @ Tue, 02 Aug 2022 12:14:39: 26000000 INFO @ Tue, 02 Aug 2022 12:14:42: 14000000 INFO @ Tue, 02 Aug 2022 12:14:43: 20000000 INFO @ Tue, 02 Aug 2022 12:14:44: 27000000 INFO @ Tue, 02 Aug 2022 12:14:47: 15000000 INFO @ Tue, 02 Aug 2022 12:14:48: 21000000 INFO @ Tue, 02 Aug 2022 12:14:49: 28000000 INFO @ Tue, 02 Aug 2022 12:14:51: 16000000 INFO @ Tue, 02 Aug 2022 12:14:53: 22000000 INFO @ Tue, 02 Aug 2022 12:14:54: 29000000 INFO @ Tue, 02 Aug 2022 12:14:56: 17000000 INFO @ Tue, 02 Aug 2022 12:14:59: 23000000 INFO @ Tue, 02 Aug 2022 12:14:59: 30000000 INFO @ Tue, 02 Aug 2022 12:15:01: 18000000 INFO @ Tue, 02 Aug 2022 12:15:04: 24000000 INFO @ Tue, 02 Aug 2022 12:15:05: 31000000 INFO @ Tue, 02 Aug 2022 12:15:06: 19000000 INFO @ Tue, 02 Aug 2022 12:15:09: 25000000 INFO @ Tue, 02 Aug 2022 12:15:10: 32000000 INFO @ Tue, 02 Aug 2022 12:15:11: 20000000 INFO @ Tue, 02 Aug 2022 12:15:14: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:15:14: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:15:14: #1 total tags in treatment: 16080739 INFO @ Tue, 02 Aug 2022 12:15:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:15:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:15:14: #1 tags after filtering in treatment: 12949916 INFO @ Tue, 02 Aug 2022 12:15:14: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 02 Aug 2022 12:15:14: #1 finished! INFO @ Tue, 02 Aug 2022 12:15:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:15:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:15:14: 26000000 INFO @ Tue, 02 Aug 2022 12:15:15: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 12:15:15: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:15:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:15:16: 21000000 INFO @ Tue, 02 Aug 2022 12:15:20: 27000000 INFO @ Tue, 02 Aug 2022 12:15:21: 22000000 INFO @ Tue, 02 Aug 2022 12:15:25: 28000000 INFO @ Tue, 02 Aug 2022 12:15:26: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:15:31: 29000000 INFO @ Tue, 02 Aug 2022 12:15:31: 24000000 INFO @ Tue, 02 Aug 2022 12:15:35: 25000000 INFO @ Tue, 02 Aug 2022 12:15:36: 30000000 INFO @ Tue, 02 Aug 2022 12:15:40: 26000000 INFO @ Tue, 02 Aug 2022 12:15:41: 31000000 INFO @ Tue, 02 Aug 2022 12:15:45: 27000000 INFO @ Tue, 02 Aug 2022 12:15:47: 32000000 INFO @ Tue, 02 Aug 2022 12:15:50: 28000000 INFO @ Tue, 02 Aug 2022 12:15:51: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:15:51: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:15:51: #1 total tags in treatment: 16080739 INFO @ Tue, 02 Aug 2022 12:15:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:15:51: #1 tags after filtering in treatment: 12949916 INFO @ Tue, 02 Aug 2022 12:15:51: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 02 Aug 2022 12:15:51: #1 finished! INFO @ Tue, 02 Aug 2022 12:15:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:15:52: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 12:15:52: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:15:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:15:55: 29000000 INFO @ Tue, 02 Aug 2022 12:15:59: 30000000 INFO @ Tue, 02 Aug 2022 12:16:04: 31000000 INFO @ Tue, 02 Aug 2022 12:16:08: 32000000 INFO @ Tue, 02 Aug 2022 12:16:12: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 12:16:12: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 12:16:12: #1 total tags in treatment: 16080739 INFO @ Tue, 02 Aug 2022 12:16:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:16:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:16:12: #1 tags after filtering in treatment: 12949916 INFO @ Tue, 02 Aug 2022 12:16:12: #1 Redundant rate of treatment: 0.19 INFO @ Tue, 02 Aug 2022 12:16:12: #1 finished! INFO @ Tue, 02 Aug 2022 12:16:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:16:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:16:13: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 12:16:13: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:16:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8948322/SRX8948322.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。