Job ID = 14170429 SRX = SRX8784758 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6131067 spots for SRR12280888/SRR12280888.sra Written 6131067 spots for SRR12280888/SRR12280888.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170823 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:36 6131067 reads; of these: 6131067 (100.00%) were unpaired; of these: 275273 (4.49%) aligned 0 times 5604697 (91.41%) aligned exactly 1 time 251097 (4.10%) aligned >1 times 95.51% overall alignment rate Time searching: 00:02:36 Overall time: 00:02:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 382257 / 5855794 = 0.0653 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:53:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:53:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:53:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:54:07: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:54:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:54:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:54:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:54:23: 1000000 INFO @ Sat, 11 Dec 2021 06:54:24: 2000000 INFO @ Sat, 11 Dec 2021 06:54:34: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:54:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:54:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:54:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:54:45: 3000000 INFO @ Sat, 11 Dec 2021 06:54:51: 3000000 INFO @ Sat, 11 Dec 2021 06:54:53: 1000000 INFO @ Sat, 11 Dec 2021 06:54:56: 4000000 INFO @ Sat, 11 Dec 2021 06:55:03: 4000000 INFO @ Sat, 11 Dec 2021 06:55:05: 2000000 INFO @ Sat, 11 Dec 2021 06:55:08: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:55:14: #1 tag size is determined as 99 bps INFO @ Sat, 11 Dec 2021 06:55:14: #1 tag size = 99 INFO @ Sat, 11 Dec 2021 06:55:14: #1 total tags in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:55:14: #1 tags after filtering in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:55:14: #1 finished! INFO @ Sat, 11 Dec 2021 06:55:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:55:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:55:14: #2 number of paired peaks: 1475 INFO @ Sat, 11 Dec 2021 06:55:14: start model_add_line... INFO @ Sat, 11 Dec 2021 06:55:14: start X-correlation... INFO @ Sat, 11 Dec 2021 06:55:14: end of X-cor INFO @ Sat, 11 Dec 2021 06:55:14: #2 finished! INFO @ Sat, 11 Dec 2021 06:55:14: #2 predicted fragment length is 249 bps INFO @ Sat, 11 Dec 2021 06:55:14: #2 alternative fragment length(s) may be 249 bps INFO @ Sat, 11 Dec 2021 06:55:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10_model.r INFO @ Sat, 11 Dec 2021 06:55:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:55:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:55:15: 5000000 INFO @ Sat, 11 Dec 2021 06:55:17: 3000000 INFO @ Sat, 11 Dec 2021 06:55:20: #1 tag size is determined as 99 bps INFO @ Sat, 11 Dec 2021 06:55:20: #1 tag size = 99 INFO @ Sat, 11 Dec 2021 06:55:20: #1 total tags in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:55:20: #1 tags after filtering in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:55:20: #1 finished! INFO @ Sat, 11 Dec 2021 06:55:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:55:20: #2 number of paired peaks: 1475 INFO @ Sat, 11 Dec 2021 06:55:20: start model_add_line... INFO @ Sat, 11 Dec 2021 06:55:20: start X-correlation... INFO @ Sat, 11 Dec 2021 06:55:20: end of X-cor INFO @ Sat, 11 Dec 2021 06:55:20: #2 finished! INFO @ Sat, 11 Dec 2021 06:55:20: #2 predicted fragment length is 249 bps INFO @ Sat, 11 Dec 2021 06:55:20: #2 alternative fragment length(s) may be 249 bps INFO @ Sat, 11 Dec 2021 06:55:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05_model.r INFO @ Sat, 11 Dec 2021 06:55:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:55:20: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:55:27: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:55:28: 4000000 INFO @ Sat, 11 Dec 2021 06:55:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10_peaks.xls INFO @ Sat, 11 Dec 2021 06:55:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:55:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.10_summits.bed INFO @ Sat, 11 Dec 2021 06:55:34: Done! INFO @ Sat, 11 Dec 2021 06:55:34: #3 Call peaks for each chromosome... pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (2739 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:55:39: 5000000 INFO @ Sat, 11 Dec 2021 06:55:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05_peaks.xls INFO @ Sat, 11 Dec 2021 06:55:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:55:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.05_summits.bed INFO @ Sat, 11 Dec 2021 06:55:41: Done! pass1 - making usageList (11 chroms): 8 millis pass2 - checking and writing primary data (6358 records, 4 fields): 30 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:55:44: #1 tag size is determined as 99 bps INFO @ Sat, 11 Dec 2021 06:55:44: #1 tag size = 99 INFO @ Sat, 11 Dec 2021 06:55:44: #1 total tags in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:55:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:55:44: #1 tags after filtering in treatment: 5473537 INFO @ Sat, 11 Dec 2021 06:55:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:55:44: #1 finished! INFO @ Sat, 11 Dec 2021 06:55:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:55:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:55:45: #2 number of paired peaks: 1475 INFO @ Sat, 11 Dec 2021 06:55:45: start model_add_line... INFO @ Sat, 11 Dec 2021 06:55:45: start X-correlation... INFO @ Sat, 11 Dec 2021 06:55:45: end of X-cor INFO @ Sat, 11 Dec 2021 06:55:45: #2 finished! INFO @ Sat, 11 Dec 2021 06:55:45: #2 predicted fragment length is 249 bps INFO @ Sat, 11 Dec 2021 06:55:45: #2 alternative fragment length(s) may be 249 bps INFO @ Sat, 11 Dec 2021 06:55:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20_model.r INFO @ Sat, 11 Dec 2021 06:55:45: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:55:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:55:57: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:56:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20_peaks.xls INFO @ Sat, 11 Dec 2021 06:56:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:56:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8784758/SRX8784758.20_summits.bed INFO @ Sat, 11 Dec 2021 06:56:04: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (853 records, 4 fields): 2 millis CompletedMACS2peakCalling