Job ID = 14172500 SRX = SRX8755939 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29246238 spots for SRR12248076/SRR12248076.sra Written 29246238 spots for SRR12248076/SRR12248076.sra fastq に変換しました。 bowtie でマッピング中... Your job 14173051 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:23 29246238 reads; of these: 29246238 (100.00%) were paired; of these: 8295676 (28.36%) aligned concordantly 0 times 18057879 (61.74%) aligned concordantly exactly 1 time 2892683 (9.89%) aligned concordantly >1 times ---- 8295676 pairs aligned concordantly 0 times; of these: 3069860 (37.01%) aligned discordantly 1 time ---- 5225816 pairs aligned 0 times concordantly or discordantly; of these: 10451632 mates make up the pairs; of these: 8265086 (79.08%) aligned 0 times 1142915 (10.94%) aligned exactly 1 time 1043631 (9.99%) aligned >1 times 85.87% overall alignment rate Time searching: 00:29:23 Overall time: 00:29:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1968444 / 23647873 = 0.0832 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:26:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:26:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:26:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:26:57: 1000000 INFO @ Sat, 11 Dec 2021 16:27:04: 2000000 INFO @ Sat, 11 Dec 2021 16:27:10: 3000000 INFO @ Sat, 11 Dec 2021 16:27:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:27:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:27:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:27:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:27:24: 5000000 INFO @ Sat, 11 Dec 2021 16:27:27: 1000000 INFO @ Sat, 11 Dec 2021 16:27:31: 6000000 INFO @ Sat, 11 Dec 2021 16:27:34: 2000000 INFO @ Sat, 11 Dec 2021 16:27:39: 7000000 INFO @ Sat, 11 Dec 2021 16:27:40: 3000000 INFO @ Sat, 11 Dec 2021 16:27:46: 8000000 INFO @ Sat, 11 Dec 2021 16:27:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:27:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:27:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:27:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:27:53: 9000000 INFO @ Sat, 11 Dec 2021 16:27:53: 5000000 INFO @ Sat, 11 Dec 2021 16:27:58: 1000000 INFO @ Sat, 11 Dec 2021 16:27:59: 6000000 INFO @ Sat, 11 Dec 2021 16:28:00: 10000000 INFO @ Sat, 11 Dec 2021 16:28:05: 2000000 INFO @ Sat, 11 Dec 2021 16:28:06: 7000000 INFO @ Sat, 11 Dec 2021 16:28:08: 11000000 INFO @ Sat, 11 Dec 2021 16:28:12: 3000000 INFO @ Sat, 11 Dec 2021 16:28:13: 8000000 INFO @ Sat, 11 Dec 2021 16:28:15: 12000000 INFO @ Sat, 11 Dec 2021 16:28:19: 4000000 INFO @ Sat, 11 Dec 2021 16:28:19: 9000000 INFO @ Sat, 11 Dec 2021 16:28:22: 13000000 INFO @ Sat, 11 Dec 2021 16:28:26: 10000000 INFO @ Sat, 11 Dec 2021 16:28:26: 5000000 INFO @ Sat, 11 Dec 2021 16:28:30: 14000000 INFO @ Sat, 11 Dec 2021 16:28:32: 11000000 INFO @ Sat, 11 Dec 2021 16:28:33: 6000000 INFO @ Sat, 11 Dec 2021 16:28:37: 15000000 INFO @ Sat, 11 Dec 2021 16:28:39: 12000000 INFO @ Sat, 11 Dec 2021 16:28:40: 7000000 INFO @ Sat, 11 Dec 2021 16:28:45: 16000000 INFO @ Sat, 11 Dec 2021 16:28:45: 13000000 INFO @ Sat, 11 Dec 2021 16:28:47: 8000000 INFO @ Sat, 11 Dec 2021 16:28:52: 14000000 INFO @ Sat, 11 Dec 2021 16:28:52: 17000000 INFO @ Sat, 11 Dec 2021 16:28:54: 9000000 INFO @ Sat, 11 Dec 2021 16:28:58: 15000000 INFO @ Sat, 11 Dec 2021 16:28:59: 18000000 INFO @ Sat, 11 Dec 2021 16:29:01: 10000000 INFO @ Sat, 11 Dec 2021 16:29:05: 16000000 INFO @ Sat, 11 Dec 2021 16:29:07: 19000000 INFO @ Sat, 11 Dec 2021 16:29:08: 11000000 INFO @ Sat, 11 Dec 2021 16:29:11: 17000000 INFO @ Sat, 11 Dec 2021 16:29:14: 20000000 INFO @ Sat, 11 Dec 2021 16:29:15: 12000000 INFO @ Sat, 11 Dec 2021 16:29:18: 18000000 INFO @ Sat, 11 Dec 2021 16:29:21: 21000000 INFO @ Sat, 11 Dec 2021 16:29:22: 13000000 INFO @ Sat, 11 Dec 2021 16:29:24: 19000000 INFO @ Sat, 11 Dec 2021 16:29:29: 22000000 INFO @ Sat, 11 Dec 2021 16:29:29: 14000000 INFO @ Sat, 11 Dec 2021 16:29:31: 20000000 INFO @ Sat, 11 Dec 2021 16:29:36: 15000000 INFO @ Sat, 11 Dec 2021 16:29:36: 23000000 INFO @ Sat, 11 Dec 2021 16:29:37: 21000000 INFO @ Sat, 11 Dec 2021 16:29:43: 16000000 INFO @ Sat, 11 Dec 2021 16:29:43: 24000000 INFO @ Sat, 11 Dec 2021 16:29:44: 22000000 INFO @ Sat, 11 Dec 2021 16:29:50: 17000000 INFO @ Sat, 11 Dec 2021 16:29:50: 23000000 INFO @ Sat, 11 Dec 2021 16:29:51: 25000000 INFO @ Sat, 11 Dec 2021 16:29:57: 24000000 INFO @ Sat, 11 Dec 2021 16:29:57: 18000000 INFO @ Sat, 11 Dec 2021 16:29:58: 26000000 INFO @ Sat, 11 Dec 2021 16:30:03: 25000000 INFO @ Sat, 11 Dec 2021 16:30:04: 19000000 INFO @ Sat, 11 Dec 2021 16:30:05: 27000000 INFO @ Sat, 11 Dec 2021 16:30:10: 26000000 INFO @ Sat, 11 Dec 2021 16:30:12: 20000000 INFO @ Sat, 11 Dec 2021 16:30:13: 28000000 INFO @ Sat, 11 Dec 2021 16:30:16: 27000000 INFO @ Sat, 11 Dec 2021 16:30:19: 21000000 INFO @ Sat, 11 Dec 2021 16:30:20: 29000000 INFO @ Sat, 11 Dec 2021 16:30:22: 28000000 INFO @ Sat, 11 Dec 2021 16:30:26: 22000000 INFO @ Sat, 11 Dec 2021 16:30:27: 30000000 INFO @ Sat, 11 Dec 2021 16:30:29: 29000000 INFO @ Sat, 11 Dec 2021 16:30:33: 23000000 INFO @ Sat, 11 Dec 2021 16:30:35: 31000000 INFO @ Sat, 11 Dec 2021 16:30:35: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:30:40: 24000000 INFO @ Sat, 11 Dec 2021 16:30:42: 32000000 INFO @ Sat, 11 Dec 2021 16:30:42: 31000000 INFO @ Sat, 11 Dec 2021 16:30:47: 25000000 INFO @ Sat, 11 Dec 2021 16:30:48: 32000000 INFO @ Sat, 11 Dec 2021 16:30:49: 33000000 INFO @ Sat, 11 Dec 2021 16:30:54: 26000000 INFO @ Sat, 11 Dec 2021 16:30:55: 33000000 INFO @ Sat, 11 Dec 2021 16:30:57: 34000000 INFO @ Sat, 11 Dec 2021 16:31:01: 34000000 INFO @ Sat, 11 Dec 2021 16:31:01: 27000000 INFO @ Sat, 11 Dec 2021 16:31:04: 35000000 INFO @ Sat, 11 Dec 2021 16:31:08: 35000000 INFO @ Sat, 11 Dec 2021 16:31:09: 28000000 INFO @ Sat, 11 Dec 2021 16:31:12: 36000000 INFO @ Sat, 11 Dec 2021 16:31:15: 36000000 INFO @ Sat, 11 Dec 2021 16:31:16: 29000000 INFO @ Sat, 11 Dec 2021 16:31:19: 37000000 INFO @ Sat, 11 Dec 2021 16:31:21: 37000000 INFO @ Sat, 11 Dec 2021 16:31:23: 30000000 INFO @ Sat, 11 Dec 2021 16:31:26: 38000000 INFO @ Sat, 11 Dec 2021 16:31:28: 38000000 INFO @ Sat, 11 Dec 2021 16:31:30: 31000000 INFO @ Sat, 11 Dec 2021 16:31:34: 39000000 INFO @ Sat, 11 Dec 2021 16:31:34: 39000000 INFO @ Sat, 11 Dec 2021 16:31:37: 32000000 INFO @ Sat, 11 Dec 2021 16:31:41: 40000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 16:31:42: 40000000 INFO @ Sat, 11 Dec 2021 16:31:44: 33000000 INFO @ Sat, 11 Dec 2021 16:31:48: 41000000 INFO @ Sat, 11 Dec 2021 16:31:49: 41000000 INFO @ Sat, 11 Dec 2021 16:31:51: 34000000 INFO @ Sat, 11 Dec 2021 16:31:56: 42000000 INFO @ Sat, 11 Dec 2021 16:31:57: 42000000 INFO @ Sat, 11 Dec 2021 16:31:59: 35000000 INFO @ Sat, 11 Dec 2021 16:32:04: 43000000 INFO @ Sat, 11 Dec 2021 16:32:04: 43000000 INFO @ Sat, 11 Dec 2021 16:32:06: 36000000 INFO @ Sat, 11 Dec 2021 16:32:11: 44000000 INFO @ Sat, 11 Dec 2021 16:32:12: 44000000 INFO @ Sat, 11 Dec 2021 16:32:13: 37000000 INFO @ Sat, 11 Dec 2021 16:32:19: 45000000 INFO @ Sat, 11 Dec 2021 16:32:19: 45000000 INFO @ Sat, 11 Dec 2021 16:32:20: 38000000 INFO @ Sat, 11 Dec 2021 16:32:26: 46000000 INFO @ Sat, 11 Dec 2021 16:32:27: 46000000 INFO @ Sat, 11 Dec 2021 16:32:28: 39000000 INFO @ Sat, 11 Dec 2021 16:32:28: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:32:28: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:32:28: #1 total tags in treatment: 19336619 INFO @ Sat, 11 Dec 2021 16:32:28: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:32:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:32:29: #1 tags after filtering in treatment: 14140963 INFO @ Sat, 11 Dec 2021 16:32:29: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:32:29: #1 finished! INFO @ Sat, 11 Dec 2021 16:32:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:32:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:32:29: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:32:29: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:32:29: #1 total tags in treatment: 19336619 INFO @ Sat, 11 Dec 2021 16:32:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:32:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:32:29: #1 tags after filtering in treatment: 14140963 INFO @ Sat, 11 Dec 2021 16:32:29: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:32:29: #1 finished! INFO @ Sat, 11 Dec 2021 16:32:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:32:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:32:30: #2 number of paired peaks: 530 WARNING @ Sat, 11 Dec 2021 16:32:30: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Sat, 11 Dec 2021 16:32:30: start model_add_line... INFO @ Sat, 11 Dec 2021 16:32:30: start X-correlation... INFO @ Sat, 11 Dec 2021 16:32:30: end of X-cor INFO @ Sat, 11 Dec 2021 16:32:30: #2 finished! INFO @ Sat, 11 Dec 2021 16:32:30: #2 predicted fragment length is 63 bps INFO @ Sat, 11 Dec 2021 16:32:30: #2 alternative fragment length(s) may be 63,572 bps INFO @ Sat, 11 Dec 2021 16:32:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05_model.r WARNING @ Sat, 11 Dec 2021 16:32:30: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:32:30: #2 You may need to consider one of the other alternative d(s): 63,572 WARNING @ Sat, 11 Dec 2021 16:32:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:32:30: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:32:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:32:30: #2 number of paired peaks: 530 WARNING @ Sat, 11 Dec 2021 16:32:30: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Sat, 11 Dec 2021 16:32:30: start model_add_line... INFO @ Sat, 11 Dec 2021 16:32:31: start X-correlation... INFO @ Sat, 11 Dec 2021 16:32:31: end of X-cor INFO @ Sat, 11 Dec 2021 16:32:31: #2 finished! INFO @ Sat, 11 Dec 2021 16:32:31: #2 predicted fragment length is 63 bps INFO @ Sat, 11 Dec 2021 16:32:31: #2 alternative fragment length(s) may be 63,572 bps INFO @ Sat, 11 Dec 2021 16:32:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10_model.r WARNING @ Sat, 11 Dec 2021 16:32:31: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:32:31: #2 You may need to consider one of the other alternative d(s): 63,572 WARNING @ Sat, 11 Dec 2021 16:32:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:32:31: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:32:31: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:32:34: 40000000 INFO @ Sat, 11 Dec 2021 16:32:41: 41000000 INFO @ Sat, 11 Dec 2021 16:32:47: 42000000 INFO @ Sat, 11 Dec 2021 16:32:53: 43000000 INFO @ Sat, 11 Dec 2021 16:32:56: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:32:58: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:32:59: 44000000 INFO @ Sat, 11 Dec 2021 16:33:06: 45000000 INFO @ Sat, 11 Dec 2021 16:33:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05_peaks.xls INFO @ Sat, 11 Dec 2021 16:33:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:33:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.05_summits.bed INFO @ Sat, 11 Dec 2021 16:33:10: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (16566 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:33:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10_peaks.xls INFO @ Sat, 11 Dec 2021 16:33:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:33:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.10_summits.bed INFO @ Sat, 11 Dec 2021 16:33:11: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7364 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:33:12: 46000000 INFO @ Sat, 11 Dec 2021 16:33:14: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:33:14: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:33:14: #1 total tags in treatment: 19336619 INFO @ Sat, 11 Dec 2021 16:33:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:33:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:33:14: #1 tags after filtering in treatment: 14140963 INFO @ Sat, 11 Dec 2021 16:33:14: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:33:14: #1 finished! INFO @ Sat, 11 Dec 2021 16:33:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:33:15: #2 number of paired peaks: 530 WARNING @ Sat, 11 Dec 2021 16:33:15: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Sat, 11 Dec 2021 16:33:15: start model_add_line... INFO @ Sat, 11 Dec 2021 16:33:15: start X-correlation... INFO @ Sat, 11 Dec 2021 16:33:15: end of X-cor INFO @ Sat, 11 Dec 2021 16:33:15: #2 finished! INFO @ Sat, 11 Dec 2021 16:33:15: #2 predicted fragment length is 63 bps INFO @ Sat, 11 Dec 2021 16:33:15: #2 alternative fragment length(s) may be 63,572 bps INFO @ Sat, 11 Dec 2021 16:33:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20_model.r WARNING @ Sat, 11 Dec 2021 16:33:15: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:33:15: #2 You may need to consider one of the other alternative d(s): 63,572 WARNING @ Sat, 11 Dec 2021 16:33:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:33:15: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:33:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:33:43: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:33:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20_peaks.xls INFO @ Sat, 11 Dec 2021 16:33:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:33:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755939/SRX8755939.20_summits.bed INFO @ Sat, 11 Dec 2021 16:33:57: Done! pass1 - making usageList (11 chroms): 0 millis pass2 - checking and writing primary data (991 records, 4 fields): 15 millis CompletedMACS2peakCalling