Job ID = 14172495 SRX = SRX8755936 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30712971 spots for SRR12248073/SRR12248073.sra Written 30712971 spots for SRR12248073/SRR12248073.sra fastq に変換しました。 bowtie でマッピング中... Your job 14173055 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:21 30712971 reads; of these: 30712971 (100.00%) were paired; of these: 9016226 (29.36%) aligned concordantly 0 times 18389710 (59.88%) aligned concordantly exactly 1 time 3307035 (10.77%) aligned concordantly >1 times ---- 9016226 pairs aligned concordantly 0 times; of these: 3247363 (36.02%) aligned discordantly 1 time ---- 5768863 pairs aligned 0 times concordantly or discordantly; of these: 11537726 mates make up the pairs; of these: 9083727 (78.73%) aligned 0 times 1237677 (10.73%) aligned exactly 1 time 1216322 (10.54%) aligned >1 times 85.21% overall alignment rate Time searching: 00:35:22 Overall time: 00:35:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2265560 / 24544129 = 0.0923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:31:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:31:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:31:26: 1000000 INFO @ Sat, 11 Dec 2021 16:31:30: 2000000 INFO @ Sat, 11 Dec 2021 16:31:34: 3000000 INFO @ Sat, 11 Dec 2021 16:31:39: 4000000 INFO @ Sat, 11 Dec 2021 16:31:43: 5000000 INFO @ Sat, 11 Dec 2021 16:31:47: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:31:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:31:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:31:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:31:52: 7000000 INFO @ Sat, 11 Dec 2021 16:31:56: 8000000 INFO @ Sat, 11 Dec 2021 16:31:57: 1000000 INFO @ Sat, 11 Dec 2021 16:32:01: 9000000 INFO @ Sat, 11 Dec 2021 16:32:02: 2000000 INFO @ Sat, 11 Dec 2021 16:32:05: 10000000 INFO @ Sat, 11 Dec 2021 16:32:08: 3000000 INFO @ Sat, 11 Dec 2021 16:32:10: 11000000 INFO @ Sat, 11 Dec 2021 16:32:13: 4000000 INFO @ Sat, 11 Dec 2021 16:32:15: 12000000 INFO @ Sat, 11 Dec 2021 16:32:18: 5000000 INFO @ Sat, 11 Dec 2021 16:32:19: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:32:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:32:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:32:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:32:24: 14000000 INFO @ Sat, 11 Dec 2021 16:32:24: 6000000 INFO @ Sat, 11 Dec 2021 16:32:26: 1000000 INFO @ Sat, 11 Dec 2021 16:32:28: 15000000 INFO @ Sat, 11 Dec 2021 16:32:29: 7000000 INFO @ Sat, 11 Dec 2021 16:32:31: 2000000 INFO @ Sat, 11 Dec 2021 16:32:33: 16000000 INFO @ Sat, 11 Dec 2021 16:32:35: 8000000 INFO @ Sat, 11 Dec 2021 16:32:35: 3000000 INFO @ Sat, 11 Dec 2021 16:32:37: 17000000 INFO @ Sat, 11 Dec 2021 16:32:40: 4000000 INFO @ Sat, 11 Dec 2021 16:32:40: 9000000 INFO @ Sat, 11 Dec 2021 16:32:42: 18000000 INFO @ Sat, 11 Dec 2021 16:32:45: 5000000 INFO @ Sat, 11 Dec 2021 16:32:46: 10000000 INFO @ Sat, 11 Dec 2021 16:32:47: 19000000 INFO @ Sat, 11 Dec 2021 16:32:49: 6000000 INFO @ Sat, 11 Dec 2021 16:32:51: 11000000 INFO @ Sat, 11 Dec 2021 16:32:51: 20000000 INFO @ Sat, 11 Dec 2021 16:32:54: 7000000 INFO @ Sat, 11 Dec 2021 16:32:56: 21000000 INFO @ Sat, 11 Dec 2021 16:32:57: 12000000 INFO @ Sat, 11 Dec 2021 16:32:59: 8000000 INFO @ Sat, 11 Dec 2021 16:33:01: 22000000 INFO @ Sat, 11 Dec 2021 16:33:02: 13000000 INFO @ Sat, 11 Dec 2021 16:33:03: 9000000 INFO @ Sat, 11 Dec 2021 16:33:05: 23000000 INFO @ Sat, 11 Dec 2021 16:33:07: 14000000 INFO @ Sat, 11 Dec 2021 16:33:08: 10000000 INFO @ Sat, 11 Dec 2021 16:33:10: 24000000 INFO @ Sat, 11 Dec 2021 16:33:13: 11000000 INFO @ Sat, 11 Dec 2021 16:33:13: 15000000 INFO @ Sat, 11 Dec 2021 16:33:14: 25000000 INFO @ Sat, 11 Dec 2021 16:33:17: 12000000 INFO @ Sat, 11 Dec 2021 16:33:18: 16000000 INFO @ Sat, 11 Dec 2021 16:33:19: 26000000 INFO @ Sat, 11 Dec 2021 16:33:22: 13000000 INFO @ Sat, 11 Dec 2021 16:33:23: 27000000 INFO @ Sat, 11 Dec 2021 16:33:24: 17000000 INFO @ Sat, 11 Dec 2021 16:33:26: 14000000 INFO @ Sat, 11 Dec 2021 16:33:28: 28000000 INFO @ Sat, 11 Dec 2021 16:33:29: 18000000 INFO @ Sat, 11 Dec 2021 16:33:31: 15000000 INFO @ Sat, 11 Dec 2021 16:33:32: 29000000 INFO @ Sat, 11 Dec 2021 16:33:35: 19000000 INFO @ Sat, 11 Dec 2021 16:33:36: 16000000 INFO @ Sat, 11 Dec 2021 16:33:37: 30000000 INFO @ Sat, 11 Dec 2021 16:33:40: 20000000 INFO @ Sat, 11 Dec 2021 16:33:40: 17000000 INFO @ Sat, 11 Dec 2021 16:33:41: 31000000 INFO @ Sat, 11 Dec 2021 16:33:45: 18000000 INFO @ Sat, 11 Dec 2021 16:33:46: 21000000 INFO @ Sat, 11 Dec 2021 16:33:46: 32000000 INFO @ Sat, 11 Dec 2021 16:33:49: 19000000 INFO @ Sat, 11 Dec 2021 16:33:50: 33000000 INFO @ Sat, 11 Dec 2021 16:33:51: 22000000 INFO @ Sat, 11 Dec 2021 16:33:54: 20000000 INFO @ Sat, 11 Dec 2021 16:33:55: 34000000 INFO @ Sat, 11 Dec 2021 16:33:56: 23000000 INFO @ Sat, 11 Dec 2021 16:33:59: 21000000 INFO @ Sat, 11 Dec 2021 16:33:59: 35000000 INFO @ Sat, 11 Dec 2021 16:34:01: 24000000 INFO @ Sat, 11 Dec 2021 16:34:03: 22000000 INFO @ Sat, 11 Dec 2021 16:34:04: 36000000 INFO @ Sat, 11 Dec 2021 16:34:06: 25000000 INFO @ Sat, 11 Dec 2021 16:34:08: 23000000 INFO @ Sat, 11 Dec 2021 16:34:09: 37000000 INFO @ Sat, 11 Dec 2021 16:34:12: 26000000 INFO @ Sat, 11 Dec 2021 16:34:12: 24000000 INFO @ Sat, 11 Dec 2021 16:34:13: 38000000 INFO @ Sat, 11 Dec 2021 16:34:17: 25000000 INFO @ Sat, 11 Dec 2021 16:34:17: 27000000 INFO @ Sat, 11 Dec 2021 16:34:18: 39000000 INFO @ Sat, 11 Dec 2021 16:34:21: 26000000 INFO @ Sat, 11 Dec 2021 16:34:22: 40000000 INFO @ Sat, 11 Dec 2021 16:34:22: 28000000 INFO @ Sat, 11 Dec 2021 16:34:26: 27000000 INFO @ Sat, 11 Dec 2021 16:34:27: 41000000 INFO @ Sat, 11 Dec 2021 16:34:27: 29000000 INFO @ Sat, 11 Dec 2021 16:34:30: 28000000 INFO @ Sat, 11 Dec 2021 16:34:31: 42000000 INFO @ Sat, 11 Dec 2021 16:34:33: 30000000 INFO @ Sat, 11 Dec 2021 16:34:35: 29000000 INFO @ Sat, 11 Dec 2021 16:34:36: 43000000 INFO @ Sat, 11 Dec 2021 16:34:38: 31000000 INFO @ Sat, 11 Dec 2021 16:34:40: 30000000 INFO @ Sat, 11 Dec 2021 16:34:40: 44000000 INFO @ Sat, 11 Dec 2021 16:34:44: 32000000 INFO @ Sat, 11 Dec 2021 16:34:44: 31000000 INFO @ Sat, 11 Dec 2021 16:34:45: 45000000 INFO @ Sat, 11 Dec 2021 16:34:49: 32000000 INFO @ Sat, 11 Dec 2021 16:34:49: 33000000 INFO @ Sat, 11 Dec 2021 16:34:49: 46000000 INFO @ Sat, 11 Dec 2021 16:34:53: 33000000 INFO @ Sat, 11 Dec 2021 16:34:54: 47000000 INFO @ Sat, 11 Dec 2021 16:34:54: 34000000 INFO @ Sat, 11 Dec 2021 16:34:58: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:34:58: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:34:58: #1 total tags in treatment: 19825184 INFO @ Sat, 11 Dec 2021 16:34:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:34:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:34:58: 34000000 INFO @ Sat, 11 Dec 2021 16:34:58: #1 tags after filtering in treatment: 14448385 INFO @ Sat, 11 Dec 2021 16:34:58: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:34:58: #1 finished! INFO @ Sat, 11 Dec 2021 16:34:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:34:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:34:59: #2 number of paired peaks: 469 WARNING @ Sat, 11 Dec 2021 16:34:59: Fewer paired peaks (469) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 469 pairs to build model! INFO @ Sat, 11 Dec 2021 16:34:59: start model_add_line... INFO @ Sat, 11 Dec 2021 16:34:59: start X-correlation... INFO @ Sat, 11 Dec 2021 16:34:59: end of X-cor INFO @ Sat, 11 Dec 2021 16:34:59: #2 finished! INFO @ Sat, 11 Dec 2021 16:34:59: #2 predicted fragment length is 53 bps INFO @ Sat, 11 Dec 2021 16:34:59: #2 alternative fragment length(s) may be 53,579 bps INFO @ Sat, 11 Dec 2021 16:34:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05_model.r WARNING @ Sat, 11 Dec 2021 16:34:59: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:34:59: #2 You may need to consider one of the other alternative d(s): 53,579 WARNING @ Sat, 11 Dec 2021 16:34:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:34:59: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:34:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:35:00: 35000000 INFO @ Sat, 11 Dec 2021 16:35:02: 35000000 INFO @ Sat, 11 Dec 2021 16:35:05: 36000000 INFO @ Sat, 11 Dec 2021 16:35:07: 36000000 INFO @ Sat, 11 Dec 2021 16:35:11: 37000000 INFO @ Sat, 11 Dec 2021 16:35:12: 37000000 INFO @ Sat, 11 Dec 2021 16:35:16: 38000000 INFO @ Sat, 11 Dec 2021 16:35:17: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:35:21: 39000000 INFO @ Sat, 11 Dec 2021 16:35:22: 39000000 INFO @ Sat, 11 Dec 2021 16:35:26: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:35:27: 40000000 INFO @ Sat, 11 Dec 2021 16:35:27: 40000000 INFO @ Sat, 11 Dec 2021 16:35:32: 41000000 INFO @ Sat, 11 Dec 2021 16:35:33: 41000000 INFO @ Sat, 11 Dec 2021 16:35:38: 42000000 INFO @ Sat, 11 Dec 2021 16:35:38: 42000000 INFO @ Sat, 11 Dec 2021 16:35:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05_peaks.xls INFO @ Sat, 11 Dec 2021 16:35:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:35:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.05_summits.bed INFO @ Sat, 11 Dec 2021 16:35:41: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (16172 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:35:43: 43000000 INFO @ Sat, 11 Dec 2021 16:35:43: 43000000 INFO @ Sat, 11 Dec 2021 16:35:48: 44000000 INFO @ Sat, 11 Dec 2021 16:35:49: 44000000 INFO @ Sat, 11 Dec 2021 16:35:53: 45000000 INFO @ Sat, 11 Dec 2021 16:35:54: 45000000 INFO @ Sat, 11 Dec 2021 16:35:59: 46000000 INFO @ Sat, 11 Dec 2021 16:35:59: 46000000 INFO @ Sat, 11 Dec 2021 16:36:04: 47000000 INFO @ Sat, 11 Dec 2021 16:36:05: 47000000 INFO @ Sat, 11 Dec 2021 16:36:09: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:36:09: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:36:09: #1 total tags in treatment: 19825184 INFO @ Sat, 11 Dec 2021 16:36:09: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:36:09: #1 tags after filtering in treatment: 14448385 INFO @ Sat, 11 Dec 2021 16:36:09: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:36:09: #1 finished! INFO @ Sat, 11 Dec 2021 16:36:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:36:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:36:09: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 16:36:09: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 16:36:09: #1 total tags in treatment: 19825184 INFO @ Sat, 11 Dec 2021 16:36:09: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:36:10: #1 tags after filtering in treatment: 14448385 INFO @ Sat, 11 Dec 2021 16:36:10: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 11 Dec 2021 16:36:10: #1 finished! INFO @ Sat, 11 Dec 2021 16:36:10: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:36:10: #2 number of paired peaks: 469 WARNING @ Sat, 11 Dec 2021 16:36:10: Fewer paired peaks (469) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 469 pairs to build model! INFO @ Sat, 11 Dec 2021 16:36:10: start model_add_line... INFO @ Sat, 11 Dec 2021 16:36:10: start X-correlation... INFO @ Sat, 11 Dec 2021 16:36:10: end of X-cor INFO @ Sat, 11 Dec 2021 16:36:10: #2 finished! INFO @ Sat, 11 Dec 2021 16:36:10: #2 predicted fragment length is 53 bps INFO @ Sat, 11 Dec 2021 16:36:10: #2 alternative fragment length(s) may be 53,579 bps INFO @ Sat, 11 Dec 2021 16:36:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10_model.r WARNING @ Sat, 11 Dec 2021 16:36:10: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:36:10: #2 You may need to consider one of the other alternative d(s): 53,579 WARNING @ Sat, 11 Dec 2021 16:36:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:36:10: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:36:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:36:11: #2 number of paired peaks: 469 WARNING @ Sat, 11 Dec 2021 16:36:11: Fewer paired peaks (469) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 469 pairs to build model! INFO @ Sat, 11 Dec 2021 16:36:11: start model_add_line... INFO @ Sat, 11 Dec 2021 16:36:11: start X-correlation... INFO @ Sat, 11 Dec 2021 16:36:11: end of X-cor INFO @ Sat, 11 Dec 2021 16:36:11: #2 finished! INFO @ Sat, 11 Dec 2021 16:36:11: #2 predicted fragment length is 53 bps INFO @ Sat, 11 Dec 2021 16:36:11: #2 alternative fragment length(s) may be 53,579 bps INFO @ Sat, 11 Dec 2021 16:36:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20_model.r WARNING @ Sat, 11 Dec 2021 16:36:11: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:36:11: #2 You may need to consider one of the other alternative d(s): 53,579 WARNING @ Sat, 11 Dec 2021 16:36:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:36:11: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:36:11: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 16:36:37: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:36:38: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:36:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10_peaks.xls INFO @ Sat, 11 Dec 2021 16:36:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:36:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.10_summits.bed INFO @ Sat, 11 Dec 2021 16:36:52: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (6558 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:36:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20_peaks.xls INFO @ Sat, 11 Dec 2021 16:36:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:36:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755936/SRX8755936.20_summits.bed INFO @ Sat, 11 Dec 2021 16:36:53: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (464 records, 4 fields): 2 millis CompletedMACS2peakCalling