Job ID = 14168090 SRX = SRX8723972 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14316633 spots for SRR12213378/SRR12213378.sra Written 14316633 spots for SRR12213378/SRR12213378.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168846 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:28 14316633 reads; of these: 14316633 (100.00%) were unpaired; of these: 1790145 (12.50%) aligned 0 times 9203379 (64.28%) aligned exactly 1 time 3323109 (23.21%) aligned >1 times 87.50% overall alignment rate Time searching: 00:04:28 Overall time: 00:04:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1164499 / 12526488 = 0.0930 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:20:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:20:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:20:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:20:47: 1000000 INFO @ Fri, 10 Dec 2021 15:20:51: 2000000 INFO @ Fri, 10 Dec 2021 15:20:56: 3000000 INFO @ Fri, 10 Dec 2021 15:21:01: 4000000 INFO @ Fri, 10 Dec 2021 15:21:05: 5000000 INFO @ Fri, 10 Dec 2021 15:21:10: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:21:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:21:12: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:21:12: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:21:14: 7000000 INFO @ Fri, 10 Dec 2021 15:21:17: 1000000 INFO @ Fri, 10 Dec 2021 15:21:19: 8000000 INFO @ Fri, 10 Dec 2021 15:21:21: 2000000 INFO @ Fri, 10 Dec 2021 15:21:24: 9000000 INFO @ Fri, 10 Dec 2021 15:21:26: 3000000 INFO @ Fri, 10 Dec 2021 15:21:28: 10000000 INFO @ Fri, 10 Dec 2021 15:21:30: 4000000 INFO @ Fri, 10 Dec 2021 15:21:33: 11000000 INFO @ Fri, 10 Dec 2021 15:21:35: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:21:35: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:21:35: #1 total tags in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:21:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:21:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:21:35: #1 tags after filtering in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:21:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:21:35: #1 finished! INFO @ Fri, 10 Dec 2021 15:21:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:21:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:21:35: 5000000 INFO @ Fri, 10 Dec 2021 15:21:36: #2 number of paired peaks: 333 WARNING @ Fri, 10 Dec 2021 15:21:36: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Fri, 10 Dec 2021 15:21:36: start model_add_line... INFO @ Fri, 10 Dec 2021 15:21:36: start X-correlation... INFO @ Fri, 10 Dec 2021 15:21:36: end of X-cor INFO @ Fri, 10 Dec 2021 15:21:36: #2 finished! INFO @ Fri, 10 Dec 2021 15:21:36: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 15:21:36: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 15:21:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05_model.r WARNING @ Fri, 10 Dec 2021 15:21:36: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:21:36: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 15:21:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:21:36: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:21:36: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:21:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:21:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:21:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:21:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:21:44: 7000000 INFO @ Fri, 10 Dec 2021 15:21:47: 1000000 INFO @ Fri, 10 Dec 2021 15:21:49: 8000000 INFO @ Fri, 10 Dec 2021 15:21:52: 2000000 INFO @ Fri, 10 Dec 2021 15:21:53: 9000000 INFO @ Fri, 10 Dec 2021 15:21:57: 3000000 INFO @ Fri, 10 Dec 2021 15:21:57: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:21:58: 10000000 INFO @ Fri, 10 Dec 2021 15:22:01: 4000000 INFO @ Fri, 10 Dec 2021 15:22:03: 11000000 INFO @ Fri, 10 Dec 2021 15:22:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:22:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:22:04: #1 total tags in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:22:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:22:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:22:05: #1 tags after filtering in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:22:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:22:05: #1 finished! INFO @ Fri, 10 Dec 2021 15:22:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:22:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:22:05: #2 number of paired peaks: 333 WARNING @ Fri, 10 Dec 2021 15:22:05: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Fri, 10 Dec 2021 15:22:05: start model_add_line... INFO @ Fri, 10 Dec 2021 15:22:05: start X-correlation... INFO @ Fri, 10 Dec 2021 15:22:05: end of X-cor INFO @ Fri, 10 Dec 2021 15:22:05: #2 finished! INFO @ Fri, 10 Dec 2021 15:22:05: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 15:22:05: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 15:22:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10_model.r WARNING @ Fri, 10 Dec 2021 15:22:05: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:22:05: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 15:22:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:22:05: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:22:05: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:22:06: 5000000 INFO @ Fri, 10 Dec 2021 15:22:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05_peaks.xls INFO @ Fri, 10 Dec 2021 15:22:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:22:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.05_summits.bed INFO @ Fri, 10 Dec 2021 15:22:08: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1568 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:22:10: 6000000 INFO @ Fri, 10 Dec 2021 15:22:15: 7000000 INFO @ Fri, 10 Dec 2021 15:22:19: 8000000 INFO @ Fri, 10 Dec 2021 15:22:24: 9000000 INFO @ Fri, 10 Dec 2021 15:22:26: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:22:28: 10000000 INFO @ Fri, 10 Dec 2021 15:22:33: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:22:35: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:22:35: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:22:35: #1 total tags in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:22:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:22:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:22:35: #1 tags after filtering in treatment: 11361989 INFO @ Fri, 10 Dec 2021 15:22:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:22:35: #1 finished! INFO @ Fri, 10 Dec 2021 15:22:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:22:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:22:36: #2 number of paired peaks: 333 WARNING @ Fri, 10 Dec 2021 15:22:36: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Fri, 10 Dec 2021 15:22:36: start model_add_line... INFO @ Fri, 10 Dec 2021 15:22:36: start X-correlation... INFO @ Fri, 10 Dec 2021 15:22:36: end of X-cor INFO @ Fri, 10 Dec 2021 15:22:36: #2 finished! INFO @ Fri, 10 Dec 2021 15:22:36: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 15:22:36: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 15:22:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20_model.r WARNING @ Fri, 10 Dec 2021 15:22:36: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:22:36: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 15:22:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:22:36: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:22:36: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:22:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10_peaks.xls INFO @ Fri, 10 Dec 2021 15:22:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:22:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.10_summits.bed INFO @ Fri, 10 Dec 2021 15:22:37: Done! pass1 - making usageList (9 chroms): 0 millis pass2 - checking and writing primary data (1291 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:22:56: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 15:23:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20_peaks.xls INFO @ Fri, 10 Dec 2021 15:23:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:23:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8723972/SRX8723972.20_summits.bed INFO @ Fri, 10 Dec 2021 15:23:07: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (966 records, 4 fields): 2 millis CompletedMACS2peakCalling