Job ID = 14172457 SRX = SRX8694702 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42736829 spots for SRR12180106/SRR12180106.sra Written 42736829 spots for SRR12180106/SRR12180106.sra fastq に変換しました。 bowtie でマッピング中... Your job 14173597 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:08:52 42736829 reads; of these: 42736829 (100.00%) were paired; of these: 12091578 (28.29%) aligned concordantly 0 times 21843324 (51.11%) aligned concordantly exactly 1 time 8801927 (20.60%) aligned concordantly >1 times ---- 12091578 pairs aligned concordantly 0 times; of these: 2021626 (16.72%) aligned discordantly 1 time ---- 10069952 pairs aligned 0 times concordantly or discordantly; of these: 20139904 mates make up the pairs; of these: 15903522 (78.97%) aligned 0 times 1604089 (7.96%) aligned exactly 1 time 2632293 (13.07%) aligned >1 times 81.39% overall alignment rate Time searching: 03:08:52 Overall time: 03:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7164928 / 32477689 = 0.2206 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:21:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:21:00: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:21:00: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:21:18: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:21:28: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:21:28: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:21:36: 2000000 INFO @ Sat, 11 Dec 2021 19:21:42: 1000000 INFO @ Sat, 11 Dec 2021 19:21:54: 3000000 INFO @ Sat, 11 Dec 2021 19:21:55: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:21:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:21:58: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:21:58: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:22:09: 3000000 INFO @ Sat, 11 Dec 2021 19:22:13: 4000000 INFO @ Sat, 11 Dec 2021 19:22:17: 1000000 INFO @ Sat, 11 Dec 2021 19:22:23: 4000000 INFO @ Sat, 11 Dec 2021 19:22:32: 5000000 INFO @ Sat, 11 Dec 2021 19:22:35: 2000000 INFO @ Sat, 11 Dec 2021 19:22:37: 5000000 INFO @ Sat, 11 Dec 2021 19:22:50: 6000000 INFO @ Sat, 11 Dec 2021 19:22:51: 6000000 INFO @ Sat, 11 Dec 2021 19:22:54: 3000000 INFO @ Sat, 11 Dec 2021 19:23:05: 7000000 INFO @ Sat, 11 Dec 2021 19:23:09: 7000000 INFO @ Sat, 11 Dec 2021 19:23:13: 4000000 INFO @ Sat, 11 Dec 2021 19:23:19: 8000000 INFO @ Sat, 11 Dec 2021 19:23:28: 8000000 INFO @ Sat, 11 Dec 2021 19:23:31: 5000000 INFO @ Sat, 11 Dec 2021 19:23:32: 9000000 INFO @ Sat, 11 Dec 2021 19:23:45: 10000000 INFO @ Sat, 11 Dec 2021 19:23:47: 9000000 INFO @ Sat, 11 Dec 2021 19:23:49: 6000000 INFO @ Sat, 11 Dec 2021 19:23:59: 11000000 INFO @ Sat, 11 Dec 2021 19:24:06: 10000000 INFO @ Sat, 11 Dec 2021 19:24:07: 7000000 INFO @ Sat, 11 Dec 2021 19:24:13: 12000000 INFO @ Sat, 11 Dec 2021 19:24:26: 8000000 INFO @ Sat, 11 Dec 2021 19:24:26: 11000000 INFO @ Sat, 11 Dec 2021 19:24:26: 13000000 INFO @ Sat, 11 Dec 2021 19:24:40: 14000000 INFO @ Sat, 11 Dec 2021 19:24:44: 9000000 INFO @ Sat, 11 Dec 2021 19:24:45: 12000000 INFO @ Sat, 11 Dec 2021 19:24:53: 15000000 INFO @ Sat, 11 Dec 2021 19:25:03: 10000000 INFO @ Sat, 11 Dec 2021 19:25:04: 13000000 INFO @ Sat, 11 Dec 2021 19:25:07: 16000000 INFO @ Sat, 11 Dec 2021 19:25:21: 17000000 INFO @ Sat, 11 Dec 2021 19:25:22: 11000000 INFO @ Sat, 11 Dec 2021 19:25:23: 14000000 INFO @ Sat, 11 Dec 2021 19:25:34: 18000000 INFO @ Sat, 11 Dec 2021 19:25:40: 12000000 INFO @ Sat, 11 Dec 2021 19:25:42: 15000000 INFO @ Sat, 11 Dec 2021 19:25:47: 19000000 INFO @ Sat, 11 Dec 2021 19:25:58: 13000000 INFO @ Sat, 11 Dec 2021 19:26:00: 16000000 INFO @ Sat, 11 Dec 2021 19:26:01: 20000000 INFO @ Sat, 11 Dec 2021 19:26:14: 21000000 INFO @ Sat, 11 Dec 2021 19:26:17: 14000000 INFO @ Sat, 11 Dec 2021 19:26:19: 17000000 INFO @ Sat, 11 Dec 2021 19:26:27: 22000000 INFO @ Sat, 11 Dec 2021 19:26:35: 15000000 INFO @ Sat, 11 Dec 2021 19:26:37: 18000000 INFO @ Sat, 11 Dec 2021 19:26:40: 23000000 INFO @ Sat, 11 Dec 2021 19:26:53: 24000000 INFO @ Sat, 11 Dec 2021 19:26:54: 16000000 INFO @ Sat, 11 Dec 2021 19:26:55: 19000000 INFO @ Sat, 11 Dec 2021 19:27:07: 25000000 INFO @ Sat, 11 Dec 2021 19:27:12: 20000000 INFO @ Sat, 11 Dec 2021 19:27:12: 17000000 INFO @ Sat, 11 Dec 2021 19:27:20: 26000000 INFO @ Sat, 11 Dec 2021 19:27:30: 21000000 INFO @ Sat, 11 Dec 2021 19:27:30: 18000000 INFO @ Sat, 11 Dec 2021 19:27:33: 27000000 INFO @ Sat, 11 Dec 2021 19:27:46: 28000000 INFO @ Sat, 11 Dec 2021 19:27:48: 19000000 INFO @ Sat, 11 Dec 2021 19:27:48: 22000000 INFO @ Sat, 11 Dec 2021 19:27:59: 29000000 INFO @ Sat, 11 Dec 2021 19:28:06: 20000000 INFO @ Sat, 11 Dec 2021 19:28:07: 23000000 INFO @ Sat, 11 Dec 2021 19:28:12: 30000000 INFO @ Sat, 11 Dec 2021 19:28:24: 21000000 INFO @ Sat, 11 Dec 2021 19:28:25: 24000000 INFO @ Sat, 11 Dec 2021 19:28:26: 31000000 INFO @ Sat, 11 Dec 2021 19:28:39: 32000000 INFO @ Sat, 11 Dec 2021 19:28:42: 22000000 INFO @ Sat, 11 Dec 2021 19:28:43: 25000000 INFO @ Sat, 11 Dec 2021 19:28:51: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 19:29:00: 23000000 INFO @ Sat, 11 Dec 2021 19:29:02: 26000000 INFO @ Sat, 11 Dec 2021 19:29:04: 34000000 INFO @ Sat, 11 Dec 2021 19:29:16: 24000000 INFO @ Sat, 11 Dec 2021 19:29:17: 35000000 INFO @ Sat, 11 Dec 2021 19:29:20: 27000000 INFO @ Sat, 11 Dec 2021 19:29:30: 36000000 INFO @ Sat, 11 Dec 2021 19:29:33: 25000000 INFO @ Sat, 11 Dec 2021 19:29:37: 28000000 INFO @ Sat, 11 Dec 2021 19:29:43: 37000000 INFO @ Sat, 11 Dec 2021 19:29:50: 26000000 INFO @ Sat, 11 Dec 2021 19:29:56: 29000000 INFO @ Sat, 11 Dec 2021 19:29:56: 38000000 INFO @ Sat, 11 Dec 2021 19:30:07: 27000000 INFO @ Sat, 11 Dec 2021 19:30:09: 39000000 INFO @ Sat, 11 Dec 2021 19:30:14: 30000000 INFO @ Sat, 11 Dec 2021 19:30:22: 40000000 INFO @ Sat, 11 Dec 2021 19:30:24: 28000000 INFO @ Sat, 11 Dec 2021 19:30:32: 31000000 INFO @ Sat, 11 Dec 2021 19:30:35: 41000000 INFO @ Sat, 11 Dec 2021 19:30:40: 29000000 INFO @ Sat, 11 Dec 2021 19:30:48: 42000000 INFO @ Sat, 11 Dec 2021 19:30:50: 32000000 INFO @ Sat, 11 Dec 2021 19:30:57: 30000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 19:31:02: 43000000 INFO @ Sat, 11 Dec 2021 19:31:07: 33000000 INFO @ Sat, 11 Dec 2021 19:31:14: 31000000 INFO @ Sat, 11 Dec 2021 19:31:15: 44000000 INFO @ Sat, 11 Dec 2021 19:31:24: 34000000 INFO @ Sat, 11 Dec 2021 19:31:28: 45000000 INFO @ Sat, 11 Dec 2021 19:31:31: 32000000 INFO @ Sat, 11 Dec 2021 19:31:41: 46000000 INFO @ Sat, 11 Dec 2021 19:31:42: 35000000 INFO @ Sat, 11 Dec 2021 19:31:47: 33000000 INFO @ Sat, 11 Dec 2021 19:31:54: 47000000 INFO @ Sat, 11 Dec 2021 19:31:59: 36000000 INFO @ Sat, 11 Dec 2021 19:32:05: 34000000 INFO @ Sat, 11 Dec 2021 19:32:07: 48000000 INFO @ Sat, 11 Dec 2021 19:32:17: 37000000 INFO @ Sat, 11 Dec 2021 19:32:20: 49000000 INFO @ Sat, 11 Dec 2021 19:32:22: 35000000 INFO @ Sat, 11 Dec 2021 19:32:33: 50000000 INFO @ Sat, 11 Dec 2021 19:32:35: 38000000 INFO @ Sat, 11 Dec 2021 19:32:39: 36000000 INFO @ Sat, 11 Dec 2021 19:32:46: 51000000 INFO @ Sat, 11 Dec 2021 19:32:53: 39000000 INFO @ Sat, 11 Dec 2021 19:32:56: 37000000 INFO @ Sat, 11 Dec 2021 19:32:59: 52000000 INFO @ Sat, 11 Dec 2021 19:33:10: 40000000 INFO @ Sat, 11 Dec 2021 19:33:12: 53000000 INFO @ Sat, 11 Dec 2021 19:33:13: 38000000 INFO @ Sat, 11 Dec 2021 19:33:25: 54000000 INFO @ Sat, 11 Dec 2021 19:33:28: 41000000 INFO @ Sat, 11 Dec 2021 19:33:30: 39000000 INFO @ Sat, 11 Dec 2021 19:33:38: 55000000 INFO @ Sat, 11 Dec 2021 19:33:41: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:33:41: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:33:41: #1 total tags in treatment: 23836468 INFO @ Sat, 11 Dec 2021 19:33:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:33:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:33:42: #1 tags after filtering in treatment: 20625697 INFO @ Sat, 11 Dec 2021 19:33:42: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 19:33:42: #1 finished! INFO @ Sat, 11 Dec 2021 19:33:42: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:33:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:33:44: #2 number of paired peaks: 743 WARNING @ Sat, 11 Dec 2021 19:33:44: Fewer paired peaks (743) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 743 pairs to build model! INFO @ Sat, 11 Dec 2021 19:33:44: start model_add_line... INFO @ Sat, 11 Dec 2021 19:33:44: start X-correlation... INFO @ Sat, 11 Dec 2021 19:33:44: end of X-cor INFO @ Sat, 11 Dec 2021 19:33:44: #2 finished! INFO @ Sat, 11 Dec 2021 19:33:44: #2 predicted fragment length is 239 bps INFO @ Sat, 11 Dec 2021 19:33:44: #2 alternative fragment length(s) may be 239 bps INFO @ Sat, 11 Dec 2021 19:33:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10_model.r WARNING @ Sat, 11 Dec 2021 19:33:44: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:33:44: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Sat, 11 Dec 2021 19:33:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:33:44: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:33:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:33:46: 42000000 INFO @ Sat, 11 Dec 2021 19:33:48: 40000000 INFO @ Sat, 11 Dec 2021 19:34:03: 43000000 INFO @ Sat, 11 Dec 2021 19:34:05: 41000000 INFO @ Sat, 11 Dec 2021 19:34:20: 44000000 INFO @ Sat, 11 Dec 2021 19:34:22: 42000000 INFO @ Sat, 11 Dec 2021 19:34:38: 45000000 INFO @ Sat, 11 Dec 2021 19:34:38: 43000000 INFO @ Sat, 11 Dec 2021 19:34:48: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:34:55: 46000000 INFO @ Sat, 11 Dec 2021 19:34:56: 44000000 INFO @ Sat, 11 Dec 2021 19:35:13: 47000000 INFO @ Sat, 11 Dec 2021 19:35:14: 45000000 INFO @ Sat, 11 Dec 2021 19:35:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10_peaks.xls INFO @ Sat, 11 Dec 2021 19:35:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:35:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.10_summits.bed INFO @ Sat, 11 Dec 2021 19:35:18: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (4252 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:35:30: 48000000 INFO @ Sat, 11 Dec 2021 19:35:30: 46000000 INFO @ Sat, 11 Dec 2021 19:35:47: 49000000 INFO @ Sat, 11 Dec 2021 19:35:47: 47000000 INFO @ Sat, 11 Dec 2021 19:36:04: 50000000 INFO @ Sat, 11 Dec 2021 19:36:04: 48000000 INFO @ Sat, 11 Dec 2021 19:36:21: 51000000 INFO @ Sat, 11 Dec 2021 19:36:21: 49000000 INFO @ Sat, 11 Dec 2021 19:36:38: 52000000 INFO @ Sat, 11 Dec 2021 19:36:38: 50000000 INFO @ Sat, 11 Dec 2021 19:36:55: 53000000 INFO @ Sat, 11 Dec 2021 19:36:55: 51000000 INFO @ Sat, 11 Dec 2021 19:37:12: 52000000 INFO @ Sat, 11 Dec 2021 19:37:13: 54000000 INFO @ Sat, 11 Dec 2021 19:37:29: 55000000 INFO @ Sat, 11 Dec 2021 19:37:29: 53000000 INFO @ Sat, 11 Dec 2021 19:37:33: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:37:33: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:37:33: #1 total tags in treatment: 23836468 INFO @ Sat, 11 Dec 2021 19:37:33: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:37:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:37:34: #1 tags after filtering in treatment: 20625697 INFO @ Sat, 11 Dec 2021 19:37:34: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 19:37:34: #1 finished! INFO @ Sat, 11 Dec 2021 19:37:34: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:37:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:37:36: #2 number of paired peaks: 743 WARNING @ Sat, 11 Dec 2021 19:37:36: Fewer paired peaks (743) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 743 pairs to build model! INFO @ Sat, 11 Dec 2021 19:37:36: start model_add_line... INFO @ Sat, 11 Dec 2021 19:37:36: start X-correlation... INFO @ Sat, 11 Dec 2021 19:37:36: end of X-cor INFO @ Sat, 11 Dec 2021 19:37:36: #2 finished! INFO @ Sat, 11 Dec 2021 19:37:36: #2 predicted fragment length is 239 bps INFO @ Sat, 11 Dec 2021 19:37:36: #2 alternative fragment length(s) may be 239 bps INFO @ Sat, 11 Dec 2021 19:37:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05_model.r WARNING @ Sat, 11 Dec 2021 19:37:36: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:37:36: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Sat, 11 Dec 2021 19:37:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:37:36: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:37:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:37:46: 54000000 INFO @ Sat, 11 Dec 2021 19:38:03: 55000000 INFO @ Sat, 11 Dec 2021 19:38:07: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:38:07: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:38:07: #1 total tags in treatment: 23836468 INFO @ Sat, 11 Dec 2021 19:38:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:38:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:38:07: #1 tags after filtering in treatment: 20625697 INFO @ Sat, 11 Dec 2021 19:38:07: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 19:38:07: #1 finished! INFO @ Sat, 11 Dec 2021 19:38:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:38:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:38:09: #2 number of paired peaks: 743 WARNING @ Sat, 11 Dec 2021 19:38:09: Fewer paired peaks (743) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 743 pairs to build model! INFO @ Sat, 11 Dec 2021 19:38:09: start model_add_line... INFO @ Sat, 11 Dec 2021 19:38:09: start X-correlation... INFO @ Sat, 11 Dec 2021 19:38:09: end of X-cor INFO @ Sat, 11 Dec 2021 19:38:09: #2 finished! INFO @ Sat, 11 Dec 2021 19:38:09: #2 predicted fragment length is 239 bps INFO @ Sat, 11 Dec 2021 19:38:09: #2 alternative fragment length(s) may be 239 bps INFO @ Sat, 11 Dec 2021 19:38:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20_model.r WARNING @ Sat, 11 Dec 2021 19:38:09: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:38:09: #2 You may need to consider one of the other alternative d(s): 239 WARNING @ Sat, 11 Dec 2021 19:38:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:38:09: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:38:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:38:41: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:39:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05_peaks.xls INFO @ Sat, 11 Dec 2021 19:39:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:39:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.05_summits.bed INFO @ Sat, 11 Dec 2021 19:39:12: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (6856 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:39:17: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:39:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20_peaks.xls INFO @ Sat, 11 Dec 2021 19:39:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:39:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694702/SRX8694702.20_summits.bed INFO @ Sat, 11 Dec 2021 19:39:49: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (2513 records, 4 fields): 9 millis CompletedMACS2peakCalling