Job ID = 14172454 SRX = SRX8694700 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36202801 spots for SRR12180108/SRR12180108.sra Written 36202801 spots for SRR12180108/SRR12180108.sra fastq に変換しました。 bowtie でマッピング中... Your job 14173593 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:09:52 36202801 reads; of these: 36202801 (100.00%) were paired; of these: 14770813 (40.80%) aligned concordantly 0 times 14992555 (41.41%) aligned concordantly exactly 1 time 6439433 (17.79%) aligned concordantly >1 times ---- 14770813 pairs aligned concordantly 0 times; of these: 3876856 (26.25%) aligned discordantly 1 time ---- 10893957 pairs aligned 0 times concordantly or discordantly; of these: 21787914 mates make up the pairs; of these: 12837479 (58.92%) aligned 0 times 4280450 (19.65%) aligned exactly 1 time 4669985 (21.43%) aligned >1 times 82.27% overall alignment rate Time searching: 03:09:52 Overall time: 03:09:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3072871 / 24036414 = 0.1278 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:17:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:17:00: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:17:00: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:17:12: 1000000 INFO @ Sat, 11 Dec 2021 19:17:24: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:17:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:17:30: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:17:30: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:17:36: 3000000 INFO @ Sat, 11 Dec 2021 19:17:45: 1000000 INFO @ Sat, 11 Dec 2021 19:17:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:18:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:18:00: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:18:00: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:18:01: 2000000 INFO @ Sat, 11 Dec 2021 19:18:01: 5000000 INFO @ Sat, 11 Dec 2021 19:18:11: 1000000 INFO @ Sat, 11 Dec 2021 19:18:13: 6000000 INFO @ Sat, 11 Dec 2021 19:18:17: 3000000 INFO @ Sat, 11 Dec 2021 19:18:21: 2000000 INFO @ Sat, 11 Dec 2021 19:18:26: 7000000 INFO @ Sat, 11 Dec 2021 19:18:31: 3000000 INFO @ Sat, 11 Dec 2021 19:18:33: 4000000 INFO @ Sat, 11 Dec 2021 19:18:38: 8000000 INFO @ Sat, 11 Dec 2021 19:18:42: 4000000 INFO @ Sat, 11 Dec 2021 19:18:49: 5000000 INFO @ Sat, 11 Dec 2021 19:18:51: 9000000 INFO @ Sat, 11 Dec 2021 19:18:52: 5000000 INFO @ Sat, 11 Dec 2021 19:19:02: 6000000 INFO @ Sat, 11 Dec 2021 19:19:03: 10000000 INFO @ Sat, 11 Dec 2021 19:19:05: 6000000 INFO @ Sat, 11 Dec 2021 19:19:12: 7000000 INFO @ Sat, 11 Dec 2021 19:19:16: 11000000 INFO @ Sat, 11 Dec 2021 19:19:21: 7000000 INFO @ Sat, 11 Dec 2021 19:19:22: 8000000 INFO @ Sat, 11 Dec 2021 19:19:28: 12000000 INFO @ Sat, 11 Dec 2021 19:19:32: 9000000 INFO @ Sat, 11 Dec 2021 19:19:37: 8000000 INFO @ Sat, 11 Dec 2021 19:19:41: 13000000 INFO @ Sat, 11 Dec 2021 19:19:42: 10000000 INFO @ Sat, 11 Dec 2021 19:19:52: 9000000 INFO @ Sat, 11 Dec 2021 19:19:52: 11000000 INFO @ Sat, 11 Dec 2021 19:19:53: 14000000 INFO @ Sat, 11 Dec 2021 19:20:02: 12000000 INFO @ Sat, 11 Dec 2021 19:20:06: 15000000 INFO @ Sat, 11 Dec 2021 19:20:07: 10000000 INFO @ Sat, 11 Dec 2021 19:20:12: 13000000 INFO @ Sat, 11 Dec 2021 19:20:18: 16000000 INFO @ Sat, 11 Dec 2021 19:20:22: 14000000 INFO @ Sat, 11 Dec 2021 19:20:23: 11000000 INFO @ Sat, 11 Dec 2021 19:20:29: 17000000 INFO @ Sat, 11 Dec 2021 19:20:32: 15000000 INFO @ Sat, 11 Dec 2021 19:20:39: 12000000 INFO @ Sat, 11 Dec 2021 19:20:41: 18000000 INFO @ Sat, 11 Dec 2021 19:20:42: 16000000 INFO @ Sat, 11 Dec 2021 19:20:52: 17000000 INFO @ Sat, 11 Dec 2021 19:20:53: 19000000 INFO @ Sat, 11 Dec 2021 19:20:55: 13000000 INFO @ Sat, 11 Dec 2021 19:21:02: 18000000 INFO @ Sat, 11 Dec 2021 19:21:04: 20000000 INFO @ Sat, 11 Dec 2021 19:21:11: 14000000 INFO @ Sat, 11 Dec 2021 19:21:12: 19000000 INFO @ Sat, 11 Dec 2021 19:21:16: 21000000 INFO @ Sat, 11 Dec 2021 19:21:22: 20000000 INFO @ Sat, 11 Dec 2021 19:21:27: 15000000 INFO @ Sat, 11 Dec 2021 19:21:27: 22000000 INFO @ Sat, 11 Dec 2021 19:21:32: 21000000 INFO @ Sat, 11 Dec 2021 19:21:38: 23000000 INFO @ Sat, 11 Dec 2021 19:21:42: 22000000 INFO @ Sat, 11 Dec 2021 19:21:42: 16000000 INFO @ Sat, 11 Dec 2021 19:21:49: 24000000 INFO @ Sat, 11 Dec 2021 19:21:52: 23000000 INFO @ Sat, 11 Dec 2021 19:21:58: 17000000 INFO @ Sat, 11 Dec 2021 19:22:01: 25000000 INFO @ Sat, 11 Dec 2021 19:22:02: 24000000 INFO @ Sat, 11 Dec 2021 19:22:12: 25000000 INFO @ Sat, 11 Dec 2021 19:22:12: 26000000 INFO @ Sat, 11 Dec 2021 19:22:15: 18000000 INFO @ Sat, 11 Dec 2021 19:22:22: 26000000 INFO @ Sat, 11 Dec 2021 19:22:23: 27000000 INFO @ Sat, 11 Dec 2021 19:22:27: 19000000 INFO @ Sat, 11 Dec 2021 19:22:32: 27000000 INFO @ Sat, 11 Dec 2021 19:22:35: 28000000 INFO @ Sat, 11 Dec 2021 19:22:39: 20000000 INFO @ Sat, 11 Dec 2021 19:22:42: 28000000 INFO @ Sat, 11 Dec 2021 19:22:46: 29000000 INFO @ Sat, 11 Dec 2021 19:22:50: 21000000 INFO @ Sat, 11 Dec 2021 19:22:52: 29000000 INFO @ Sat, 11 Dec 2021 19:22:58: 30000000 INFO @ Sat, 11 Dec 2021 19:23:02: 30000000 INFO @ Sat, 11 Dec 2021 19:23:02: 22000000 INFO @ Sat, 11 Dec 2021 19:23:11: 31000000 INFO @ Sat, 11 Dec 2021 19:23:11: 31000000 INFO @ Sat, 11 Dec 2021 19:23:15: 23000000 INFO @ Sat, 11 Dec 2021 19:23:21: 32000000 INFO @ Sat, 11 Dec 2021 19:23:22: 32000000 INFO @ Sat, 11 Dec 2021 19:23:28: 24000000 INFO @ Sat, 11 Dec 2021 19:23:31: 33000000 INFO @ Sat, 11 Dec 2021 19:23:35: 33000000 INFO @ Sat, 11 Dec 2021 19:23:39: 25000000 INFO @ Sat, 11 Dec 2021 19:23:41: 34000000 INFO @ Sat, 11 Dec 2021 19:23:47: 34000000 INFO @ Sat, 11 Dec 2021 19:23:51: 26000000 INFO @ Sat, 11 Dec 2021 19:23:51: 35000000 INFO @ Sat, 11 Dec 2021 19:24:00: 35000000 INFO @ Sat, 11 Dec 2021 19:24:01: 36000000 INFO @ Sat, 11 Dec 2021 19:24:02: 27000000 INFO @ Sat, 11 Dec 2021 19:24:11: 37000000 INFO @ Sat, 11 Dec 2021 19:24:13: 36000000 INFO @ Sat, 11 Dec 2021 19:24:13: 28000000 INFO @ Sat, 11 Dec 2021 19:24:21: 38000000 INFO @ Sat, 11 Dec 2021 19:24:25: 29000000 INFO @ Sat, 11 Dec 2021 19:24:25: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 19:24:31: 39000000 INFO @ Sat, 11 Dec 2021 19:24:37: 30000000 INFO @ Sat, 11 Dec 2021 19:24:38: 38000000 INFO @ Sat, 11 Dec 2021 19:24:41: 40000000 INFO @ Sat, 11 Dec 2021 19:24:50: 31000000 INFO @ Sat, 11 Dec 2021 19:24:50: 39000000 INFO @ Sat, 11 Dec 2021 19:24:51: 41000000 INFO @ Sat, 11 Dec 2021 19:25:01: 42000000 INFO @ Sat, 11 Dec 2021 19:25:02: 40000000 INFO @ Sat, 11 Dec 2021 19:25:02: 32000000 INFO @ Sat, 11 Dec 2021 19:25:11: 43000000 INFO @ Sat, 11 Dec 2021 19:25:13: 33000000 INFO @ Sat, 11 Dec 2021 19:25:14: 41000000 INFO @ Sat, 11 Dec 2021 19:25:21: 44000000 INFO @ Sat, 11 Dec 2021 19:25:25: 34000000 INFO @ Sat, 11 Dec 2021 19:25:27: 42000000 INFO @ Sat, 11 Dec 2021 19:25:31: 45000000 INFO @ Sat, 11 Dec 2021 19:25:37: 35000000 INFO @ Sat, 11 Dec 2021 19:25:41: 43000000 INFO @ Sat, 11 Dec 2021 19:25:42: 46000000 INFO @ Sat, 11 Dec 2021 19:25:50: 36000000 INFO @ Sat, 11 Dec 2021 19:25:52: 47000000 INFO @ Sat, 11 Dec 2021 19:25:53: 44000000 INFO @ Sat, 11 Dec 2021 19:26:02: 37000000 INFO @ Sat, 11 Dec 2021 19:26:02: 48000000 INFO @ Sat, 11 Dec 2021 19:26:07: 45000000 INFO @ Sat, 11 Dec 2021 19:26:12: 49000000 INFO @ Sat, 11 Dec 2021 19:26:13: 38000000 INFO @ Sat, 11 Dec 2021 19:26:21: 46000000 INFO @ Sat, 11 Dec 2021 19:26:22: 50000000 INFO @ Sat, 11 Dec 2021 19:26:25: 39000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 19:26:32: 51000000 INFO @ Sat, 11 Dec 2021 19:26:34: 47000000 INFO @ Sat, 11 Dec 2021 19:26:38: 40000000 INFO @ Sat, 11 Dec 2021 19:26:42: 52000000 INFO @ Sat, 11 Dec 2021 19:26:48: 48000000 INFO @ Sat, 11 Dec 2021 19:26:50: 41000000 INFO @ Sat, 11 Dec 2021 19:26:53: 53000000 INFO @ Sat, 11 Dec 2021 19:26:57: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:26:57: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:26:57: #1 total tags in treatment: 18649140 INFO @ Sat, 11 Dec 2021 19:26:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:26:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:26:58: #1 tags after filtering in treatment: 16658091 INFO @ Sat, 11 Dec 2021 19:26:58: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 19:26:58: #1 finished! INFO @ Sat, 11 Dec 2021 19:26:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:26:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:26:59: #2 number of paired peaks: 724 WARNING @ Sat, 11 Dec 2021 19:26:59: Fewer paired peaks (724) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 724 pairs to build model! INFO @ Sat, 11 Dec 2021 19:26:59: start model_add_line... INFO @ Sat, 11 Dec 2021 19:26:59: start X-correlation... INFO @ Sat, 11 Dec 2021 19:26:59: end of X-cor INFO @ Sat, 11 Dec 2021 19:26:59: #2 finished! INFO @ Sat, 11 Dec 2021 19:26:59: #2 predicted fragment length is 198 bps INFO @ Sat, 11 Dec 2021 19:26:59: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 11 Dec 2021 19:26:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20_model.r WARNING @ Sat, 11 Dec 2021 19:26:59: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:26:59: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 11 Dec 2021 19:26:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:26:59: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:26:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:27:01: 49000000 INFO @ Sat, 11 Dec 2021 19:27:03: 42000000 INFO @ Sat, 11 Dec 2021 19:27:14: 50000000 INFO @ Sat, 11 Dec 2021 19:27:16: 43000000 INFO @ Sat, 11 Dec 2021 19:27:27: 51000000 INFO @ Sat, 11 Dec 2021 19:27:28: 44000000 INFO @ Sat, 11 Dec 2021 19:27:39: 52000000 INFO @ Sat, 11 Dec 2021 19:27:41: 45000000 INFO @ Sat, 11 Dec 2021 19:27:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:27:52: 53000000 INFO @ Sat, 11 Dec 2021 19:27:54: 46000000 INFO @ Sat, 11 Dec 2021 19:27:58: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:27:58: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:27:58: #1 total tags in treatment: 18649140 INFO @ Sat, 11 Dec 2021 19:27:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:27:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:27:58: #1 tags after filtering in treatment: 16658091 INFO @ Sat, 11 Dec 2021 19:27:58: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 19:27:58: #1 finished! INFO @ Sat, 11 Dec 2021 19:27:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:27:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:27:59: #2 number of paired peaks: 724 WARNING @ Sat, 11 Dec 2021 19:27:59: Fewer paired peaks (724) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 724 pairs to build model! INFO @ Sat, 11 Dec 2021 19:27:59: start model_add_line... INFO @ Sat, 11 Dec 2021 19:28:00: start X-correlation... INFO @ Sat, 11 Dec 2021 19:28:00: end of X-cor INFO @ Sat, 11 Dec 2021 19:28:00: #2 finished! INFO @ Sat, 11 Dec 2021 19:28:00: #2 predicted fragment length is 198 bps INFO @ Sat, 11 Dec 2021 19:28:00: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 11 Dec 2021 19:28:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05_model.r WARNING @ Sat, 11 Dec 2021 19:28:00: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:28:00: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 11 Dec 2021 19:28:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:28:00: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:28:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:28:05: 47000000 INFO @ Sat, 11 Dec 2021 19:28:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20_peaks.xls INFO @ Sat, 11 Dec 2021 19:28:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:28:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.20_summits.bed INFO @ Sat, 11 Dec 2021 19:28:11: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (1263 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:28:17: 48000000 INFO @ Sat, 11 Dec 2021 19:28:28: 49000000 INFO @ Sat, 11 Dec 2021 19:28:39: 50000000 INFO @ Sat, 11 Dec 2021 19:28:49: 51000000 INFO @ Sat, 11 Dec 2021 19:28:50: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:29:00: 52000000 INFO @ Sat, 11 Dec 2021 19:29:11: 53000000 INFO @ Sat, 11 Dec 2021 19:29:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05_peaks.xls INFO @ Sat, 11 Dec 2021 19:29:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:29:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.05_summits.bed INFO @ Sat, 11 Dec 2021 19:29:13: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3182 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:29:16: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 19:29:16: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 19:29:16: #1 total tags in treatment: 18649140 INFO @ Sat, 11 Dec 2021 19:29:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:29:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:29:16: #1 tags after filtering in treatment: 16658091 INFO @ Sat, 11 Dec 2021 19:29:16: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 19:29:16: #1 finished! INFO @ Sat, 11 Dec 2021 19:29:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:29:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:29:18: #2 number of paired peaks: 724 WARNING @ Sat, 11 Dec 2021 19:29:18: Fewer paired peaks (724) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 724 pairs to build model! INFO @ Sat, 11 Dec 2021 19:29:18: start model_add_line... INFO @ Sat, 11 Dec 2021 19:29:18: start X-correlation... INFO @ Sat, 11 Dec 2021 19:29:18: end of X-cor INFO @ Sat, 11 Dec 2021 19:29:18: #2 finished! INFO @ Sat, 11 Dec 2021 19:29:18: #2 predicted fragment length is 198 bps INFO @ Sat, 11 Dec 2021 19:29:18: #2 alternative fragment length(s) may be 198 bps INFO @ Sat, 11 Dec 2021 19:29:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10_model.r WARNING @ Sat, 11 Dec 2021 19:29:18: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:29:18: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Sat, 11 Dec 2021 19:29:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:29:18: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:29:18: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:30:08: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:30:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10_peaks.xls INFO @ Sat, 11 Dec 2021 19:30:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:30:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694700/SRX8694700.10_summits.bed INFO @ Sat, 11 Dec 2021 19:30:30: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (2139 records, 4 fields): 5 millis CompletedMACS2peakCalling