Job ID = 14167041 SRX = SRX8694698 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 47065045 spots for SRR12180110/SRR12180110.sra Written 47065045 spots for SRR12180110/SRR12180110.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168275 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 04:05:56 47065045 reads; of these: 47065045 (100.00%) were paired; of these: 14381258 (30.56%) aligned concordantly 0 times 23100343 (49.08%) aligned concordantly exactly 1 time 9583444 (20.36%) aligned concordantly >1 times ---- 14381258 pairs aligned concordantly 0 times; of these: 5021641 (34.92%) aligned discordantly 1 time ---- 9359617 pairs aligned 0 times concordantly or discordantly; of these: 18719234 mates make up the pairs; of these: 9622209 (51.40%) aligned 0 times 3852151 (20.58%) aligned exactly 1 time 5244874 (28.02%) aligned >1 times 89.78% overall alignment rate Time searching: 04:05:56 Overall time: 04:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 64 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4290622 / 36230912 = 0.1184 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:06:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:06:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:06:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:06:29: 1000000 INFO @ Fri, 10 Dec 2021 14:06:42: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:06:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:06:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:06:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:06:56: 3000000 INFO @ Fri, 10 Dec 2021 14:06:59: 1000000 INFO @ Fri, 10 Dec 2021 14:07:09: 4000000 INFO @ Fri, 10 Dec 2021 14:07:12: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:07:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:07:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:07:23: 5000000 INFO @ Fri, 10 Dec 2021 14:07:25: 3000000 INFO @ Fri, 10 Dec 2021 14:07:33: 1000000 INFO @ Fri, 10 Dec 2021 14:07:37: 6000000 INFO @ Fri, 10 Dec 2021 14:07:38: 4000000 INFO @ Fri, 10 Dec 2021 14:07:51: 2000000 INFO @ Fri, 10 Dec 2021 14:07:51: 5000000 INFO @ Fri, 10 Dec 2021 14:07:51: 7000000 INFO @ Fri, 10 Dec 2021 14:08:04: 6000000 INFO @ Fri, 10 Dec 2021 14:08:06: 8000000 INFO @ Fri, 10 Dec 2021 14:08:08: 3000000 INFO @ Fri, 10 Dec 2021 14:08:18: 7000000 INFO @ Fri, 10 Dec 2021 14:08:20: 9000000 INFO @ Fri, 10 Dec 2021 14:08:25: 4000000 INFO @ Fri, 10 Dec 2021 14:08:31: 8000000 INFO @ Fri, 10 Dec 2021 14:08:33: 10000000 INFO @ Fri, 10 Dec 2021 14:08:42: 5000000 INFO @ Fri, 10 Dec 2021 14:08:44: 9000000 INFO @ Fri, 10 Dec 2021 14:08:47: 11000000 INFO @ Fri, 10 Dec 2021 14:08:57: 10000000 INFO @ Fri, 10 Dec 2021 14:08:59: 6000000 INFO @ Fri, 10 Dec 2021 14:09:00: 12000000 INFO @ Fri, 10 Dec 2021 14:09:09: 11000000 INFO @ Fri, 10 Dec 2021 14:09:14: 13000000 INFO @ Fri, 10 Dec 2021 14:09:17: 7000000 INFO @ Fri, 10 Dec 2021 14:09:22: 12000000 INFO @ Fri, 10 Dec 2021 14:09:28: 14000000 INFO @ Fri, 10 Dec 2021 14:09:34: 8000000 INFO @ Fri, 10 Dec 2021 14:09:35: 13000000 INFO @ Fri, 10 Dec 2021 14:09:41: 15000000 INFO @ Fri, 10 Dec 2021 14:09:49: 14000000 INFO @ Fri, 10 Dec 2021 14:09:51: 9000000 INFO @ Fri, 10 Dec 2021 14:09:55: 16000000 INFO @ Fri, 10 Dec 2021 14:10:02: 15000000 INFO @ Fri, 10 Dec 2021 14:10:08: 10000000 INFO @ Fri, 10 Dec 2021 14:10:09: 17000000 INFO @ Fri, 10 Dec 2021 14:10:15: 16000000 INFO @ Fri, 10 Dec 2021 14:10:23: 18000000 INFO @ Fri, 10 Dec 2021 14:10:24: 11000000 INFO @ Fri, 10 Dec 2021 14:10:28: 17000000 INFO @ Fri, 10 Dec 2021 14:10:37: 19000000 INFO @ Fri, 10 Dec 2021 14:10:41: 12000000 INFO @ Fri, 10 Dec 2021 14:10:41: 18000000 INFO @ Fri, 10 Dec 2021 14:10:50: 20000000 INFO @ Fri, 10 Dec 2021 14:10:55: 19000000 INFO @ Fri, 10 Dec 2021 14:10:57: 13000000 INFO @ Fri, 10 Dec 2021 14:11:04: 21000000 INFO @ Fri, 10 Dec 2021 14:11:08: 20000000 INFO @ Fri, 10 Dec 2021 14:11:14: 14000000 INFO @ Fri, 10 Dec 2021 14:11:18: 22000000 INFO @ Fri, 10 Dec 2021 14:11:21: 21000000 INFO @ Fri, 10 Dec 2021 14:11:31: 15000000 INFO @ Fri, 10 Dec 2021 14:11:32: 23000000 INFO @ Fri, 10 Dec 2021 14:11:35: 22000000 INFO @ Fri, 10 Dec 2021 14:11:45: 24000000 INFO @ Fri, 10 Dec 2021 14:11:47: 16000000 INFO @ Fri, 10 Dec 2021 14:11:48: 23000000 INFO @ Fri, 10 Dec 2021 14:11:59: 25000000 INFO @ Fri, 10 Dec 2021 14:12:01: 24000000 INFO @ Fri, 10 Dec 2021 14:12:05: 17000000 INFO @ Fri, 10 Dec 2021 14:12:12: 26000000 INFO @ Fri, 10 Dec 2021 14:12:14: 25000000 INFO @ Fri, 10 Dec 2021 14:12:22: 18000000 INFO @ Fri, 10 Dec 2021 14:12:26: 27000000 INFO @ Fri, 10 Dec 2021 14:12:27: 26000000 INFO @ Fri, 10 Dec 2021 14:12:38: 19000000 INFO @ Fri, 10 Dec 2021 14:12:40: 28000000 INFO @ Fri, 10 Dec 2021 14:12:41: 27000000 INFO @ Fri, 10 Dec 2021 14:12:54: 29000000 INFO @ Fri, 10 Dec 2021 14:12:54: 28000000 INFO @ Fri, 10 Dec 2021 14:12:55: 20000000 INFO @ Fri, 10 Dec 2021 14:13:07: 29000000 INFO @ Fri, 10 Dec 2021 14:13:07: 30000000 INFO @ Fri, 10 Dec 2021 14:13:12: 21000000 INFO @ Fri, 10 Dec 2021 14:13:19: 30000000 INFO @ Fri, 10 Dec 2021 14:13:21: 31000000 INFO @ Fri, 10 Dec 2021 14:13:29: 22000000 INFO @ Fri, 10 Dec 2021 14:13:32: 31000000 INFO @ Fri, 10 Dec 2021 14:13:35: 32000000 INFO @ Fri, 10 Dec 2021 14:13:45: 32000000 INFO @ Fri, 10 Dec 2021 14:13:45: 23000000 INFO @ Fri, 10 Dec 2021 14:13:49: 33000000 INFO @ Fri, 10 Dec 2021 14:13:57: 33000000 INFO @ Fri, 10 Dec 2021 14:14:01: 24000000 INFO @ Fri, 10 Dec 2021 14:14:04: 34000000 INFO @ Fri, 10 Dec 2021 14:14:10: 34000000 INFO @ Fri, 10 Dec 2021 14:14:17: 25000000 INFO @ Fri, 10 Dec 2021 14:14:18: 35000000 INFO @ Fri, 10 Dec 2021 14:14:23: 35000000 INFO @ Fri, 10 Dec 2021 14:14:32: 36000000 INFO @ Fri, 10 Dec 2021 14:14:34: 26000000 INFO @ Fri, 10 Dec 2021 14:14:36: 36000000 INFO @ Fri, 10 Dec 2021 14:14:45: 37000000 INFO @ Fri, 10 Dec 2021 14:14:49: 37000000 INFO @ Fri, 10 Dec 2021 14:14:50: 27000000 INFO @ Fri, 10 Dec 2021 14:14:58: 38000000 INFO @ Fri, 10 Dec 2021 14:15:01: 38000000 INFO @ Fri, 10 Dec 2021 14:15:07: 28000000 INFO @ Fri, 10 Dec 2021 14:15:11: 39000000 INFO @ Fri, 10 Dec 2021 14:15:14: 39000000 INFO @ Fri, 10 Dec 2021 14:15:24: 29000000 INFO @ Fri, 10 Dec 2021 14:15:25: 40000000 INFO @ Fri, 10 Dec 2021 14:15:26: 40000000 INFO @ Fri, 10 Dec 2021 14:15:39: 41000000 INFO @ Fri, 10 Dec 2021 14:15:39: 41000000 INFO @ Fri, 10 Dec 2021 14:15:41: 30000000 INFO @ Fri, 10 Dec 2021 14:15:52: 42000000 INFO @ Fri, 10 Dec 2021 14:15:52: 42000000 INFO @ Fri, 10 Dec 2021 14:15:58: 31000000 INFO @ Fri, 10 Dec 2021 14:16:05: 43000000 INFO @ Fri, 10 Dec 2021 14:16:06: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 14:16:15: 32000000 INFO @ Fri, 10 Dec 2021 14:16:18: 44000000 INFO @ Fri, 10 Dec 2021 14:16:20: 44000000 INFO @ Fri, 10 Dec 2021 14:16:30: 45000000 INFO @ Fri, 10 Dec 2021 14:16:32: 33000000 INFO @ Fri, 10 Dec 2021 14:16:33: 45000000 INFO @ Fri, 10 Dec 2021 14:16:42: 46000000 INFO @ Fri, 10 Dec 2021 14:16:46: 46000000 INFO @ Fri, 10 Dec 2021 14:16:49: 34000000 INFO @ Fri, 10 Dec 2021 14:16:55: 47000000 INFO @ Fri, 10 Dec 2021 14:16:58: 47000000 INFO @ Fri, 10 Dec 2021 14:17:06: 35000000 INFO @ Fri, 10 Dec 2021 14:17:07: 48000000 INFO @ Fri, 10 Dec 2021 14:17:11: 48000000 INFO @ Fri, 10 Dec 2021 14:17:19: 49000000 INFO @ Fri, 10 Dec 2021 14:17:22: 36000000 INFO @ Fri, 10 Dec 2021 14:17:24: 49000000 INFO @ Fri, 10 Dec 2021 14:17:31: 50000000 INFO @ Fri, 10 Dec 2021 14:17:36: 50000000 INFO @ Fri, 10 Dec 2021 14:17:39: 37000000 INFO @ Fri, 10 Dec 2021 14:17:43: 51000000 INFO @ Fri, 10 Dec 2021 14:17:49: 51000000 INFO @ Fri, 10 Dec 2021 14:17:55: 52000000 INFO @ Fri, 10 Dec 2021 14:17:55: 38000000 INFO @ Fri, 10 Dec 2021 14:18:02: 52000000 INFO @ Fri, 10 Dec 2021 14:18:06: 53000000 INFO @ Fri, 10 Dec 2021 14:18:11: 39000000 INFO @ Fri, 10 Dec 2021 14:18:15: 53000000 INFO @ Fri, 10 Dec 2021 14:18:18: 54000000 INFO @ Fri, 10 Dec 2021 14:18:28: 40000000 INFO @ Fri, 10 Dec 2021 14:18:28: 54000000 INFO @ Fri, 10 Dec 2021 14:18:30: 55000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 14:18:41: 55000000 INFO @ Fri, 10 Dec 2021 14:18:43: 56000000 INFO @ Fri, 10 Dec 2021 14:18:44: 41000000 INFO @ Fri, 10 Dec 2021 14:18:54: 56000000 INFO @ Fri, 10 Dec 2021 14:18:55: 57000000 INFO @ Fri, 10 Dec 2021 14:19:01: 42000000 INFO @ Fri, 10 Dec 2021 14:19:07: 58000000 INFO @ Fri, 10 Dec 2021 14:19:08: 57000000 INFO @ Fri, 10 Dec 2021 14:19:18: 43000000 INFO @ Fri, 10 Dec 2021 14:19:19: 59000000 INFO @ Fri, 10 Dec 2021 14:19:20: 58000000 INFO @ Fri, 10 Dec 2021 14:19:32: 60000000 INFO @ Fri, 10 Dec 2021 14:19:33: 59000000 INFO @ Fri, 10 Dec 2021 14:19:34: 44000000 INFO @ Fri, 10 Dec 2021 14:19:44: 61000000 INFO @ Fri, 10 Dec 2021 14:19:46: 60000000 INFO @ Fri, 10 Dec 2021 14:19:50: 45000000 INFO @ Fri, 10 Dec 2021 14:19:56: 62000000 INFO @ Fri, 10 Dec 2021 14:19:59: 61000000 INFO @ Fri, 10 Dec 2021 14:20:06: 46000000 INFO @ Fri, 10 Dec 2021 14:20:09: 63000000 INFO @ Fri, 10 Dec 2021 14:20:12: 62000000 INFO @ Fri, 10 Dec 2021 14:20:21: 47000000 INFO @ Fri, 10 Dec 2021 14:20:21: 64000000 INFO @ Fri, 10 Dec 2021 14:20:25: 63000000 INFO @ Fri, 10 Dec 2021 14:20:34: 65000000 INFO @ Fri, 10 Dec 2021 14:20:37: 48000000 INFO @ Fri, 10 Dec 2021 14:20:38: 64000000 INFO @ Fri, 10 Dec 2021 14:20:47: 66000000 INFO @ Fri, 10 Dec 2021 14:20:52: 65000000 INFO @ Fri, 10 Dec 2021 14:20:53: 49000000 INFO @ Fri, 10 Dec 2021 14:21:00: 67000000 INFO @ Fri, 10 Dec 2021 14:21:05: 66000000 INFO @ Fri, 10 Dec 2021 14:21:09: 50000000 INFO @ Fri, 10 Dec 2021 14:21:13: 68000000 INFO @ Fri, 10 Dec 2021 14:21:18: 67000000 INFO @ Fri, 10 Dec 2021 14:21:24: 51000000 INFO @ Fri, 10 Dec 2021 14:21:26: 69000000 INFO @ Fri, 10 Dec 2021 14:21:32: 68000000 INFO @ Fri, 10 Dec 2021 14:21:38: 70000000 INFO @ Fri, 10 Dec 2021 14:21:40: 52000000 INFO @ Fri, 10 Dec 2021 14:21:45: 69000000 INFO @ Fri, 10 Dec 2021 14:21:51: 71000000 INFO @ Fri, 10 Dec 2021 14:21:55: 53000000 INFO @ Fri, 10 Dec 2021 14:21:58: 70000000 INFO @ Fri, 10 Dec 2021 14:22:04: 72000000 INFO @ Fri, 10 Dec 2021 14:22:10: 54000000 INFO @ Fri, 10 Dec 2021 14:22:11: 71000000 INFO @ Fri, 10 Dec 2021 14:22:17: 73000000 INFO @ Fri, 10 Dec 2021 14:22:24: 72000000 INFO @ Fri, 10 Dec 2021 14:22:26: 55000000 INFO @ Fri, 10 Dec 2021 14:22:29: 74000000 INFO @ Fri, 10 Dec 2021 14:22:37: 73000000 INFO @ Fri, 10 Dec 2021 14:22:41: 56000000 INFO @ Fri, 10 Dec 2021 14:22:42: 75000000 INFO @ Fri, 10 Dec 2021 14:22:50: 74000000 INFO @ Fri, 10 Dec 2021 14:22:54: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 14:22:54: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 14:22:54: #1 total tags in treatment: 28762062 INFO @ Fri, 10 Dec 2021 14:22:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:22:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:22:54: #1 tags after filtering in treatment: 25166522 INFO @ Fri, 10 Dec 2021 14:22:54: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 14:22:54: #1 finished! INFO @ Fri, 10 Dec 2021 14:22:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:22:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:22:56: 57000000 INFO @ Fri, 10 Dec 2021 14:22:56: #2 number of paired peaks: 483 WARNING @ Fri, 10 Dec 2021 14:22:56: Fewer paired peaks (483) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 483 pairs to build model! INFO @ Fri, 10 Dec 2021 14:22:56: start model_add_line... INFO @ Fri, 10 Dec 2021 14:22:57: start X-correlation... INFO @ Fri, 10 Dec 2021 14:22:57: end of X-cor INFO @ Fri, 10 Dec 2021 14:22:57: #2 finished! INFO @ Fri, 10 Dec 2021 14:22:57: #2 predicted fragment length is 195 bps INFO @ Fri, 10 Dec 2021 14:22:57: #2 alternative fragment length(s) may be 195 bps INFO @ Fri, 10 Dec 2021 14:22:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10_model.r WARNING @ Fri, 10 Dec 2021 14:22:57: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:22:57: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Fri, 10 Dec 2021 14:22:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:22:57: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:22:57: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:23:02: 75000000 INFO @ Fri, 10 Dec 2021 14:23:11: 58000000 INFO @ Fri, 10 Dec 2021 14:23:14: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 14:23:14: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 14:23:14: #1 total tags in treatment: 28762062 INFO @ Fri, 10 Dec 2021 14:23:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:23:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:23:15: #1 tags after filtering in treatment: 25166522 INFO @ Fri, 10 Dec 2021 14:23:15: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 14:23:15: #1 finished! INFO @ Fri, 10 Dec 2021 14:23:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:23:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:23:17: #2 number of paired peaks: 483 WARNING @ Fri, 10 Dec 2021 14:23:17: Fewer paired peaks (483) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 483 pairs to build model! INFO @ Fri, 10 Dec 2021 14:23:17: start model_add_line... INFO @ Fri, 10 Dec 2021 14:23:17: start X-correlation... INFO @ Fri, 10 Dec 2021 14:23:17: end of X-cor INFO @ Fri, 10 Dec 2021 14:23:17: #2 finished! INFO @ Fri, 10 Dec 2021 14:23:17: #2 predicted fragment length is 195 bps INFO @ Fri, 10 Dec 2021 14:23:17: #2 alternative fragment length(s) may be 195 bps INFO @ Fri, 10 Dec 2021 14:23:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05_model.r WARNING @ Fri, 10 Dec 2021 14:23:17: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:23:17: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Fri, 10 Dec 2021 14:23:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:23:17: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:23:17: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:23:26: 59000000 INFO @ Fri, 10 Dec 2021 14:23:41: 60000000 INFO @ Fri, 10 Dec 2021 14:23:56: 61000000 INFO @ Fri, 10 Dec 2021 14:24:06: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:24:11: 62000000 INFO @ Fri, 10 Dec 2021 14:24:26: 63000000 INFO @ Fri, 10 Dec 2021 14:24:26: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:24:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10_peaks.xls INFO @ Fri, 10 Dec 2021 14:24:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:24:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.10_summits.bed INFO @ Fri, 10 Dec 2021 14:24:36: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (2386 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:24:41: 64000000 INFO @ Fri, 10 Dec 2021 14:24:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05_peaks.xls INFO @ Fri, 10 Dec 2021 14:24:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:24:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.05_summits.bed INFO @ Fri, 10 Dec 2021 14:24:56: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3681 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:24:57: 65000000 INFO @ Fri, 10 Dec 2021 14:25:12: 66000000 INFO @ Fri, 10 Dec 2021 14:25:27: 67000000 INFO @ Fri, 10 Dec 2021 14:25:43: 68000000 INFO @ Fri, 10 Dec 2021 14:25:58: 69000000 INFO @ Fri, 10 Dec 2021 14:26:13: 70000000 INFO @ Fri, 10 Dec 2021 14:26:29: 71000000 INFO @ Fri, 10 Dec 2021 14:26:44: 72000000 INFO @ Fri, 10 Dec 2021 14:27:00: 73000000 INFO @ Fri, 10 Dec 2021 14:27:15: 74000000 INFO @ Fri, 10 Dec 2021 14:27:30: 75000000 INFO @ Fri, 10 Dec 2021 14:27:44: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 14:27:44: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 14:27:44: #1 total tags in treatment: 28762062 INFO @ Fri, 10 Dec 2021 14:27:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:27:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:27:44: #1 tags after filtering in treatment: 25166522 INFO @ Fri, 10 Dec 2021 14:27:44: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 14:27:44: #1 finished! INFO @ Fri, 10 Dec 2021 14:27:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:27:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:27:47: #2 number of paired peaks: 483 WARNING @ Fri, 10 Dec 2021 14:27:47: Fewer paired peaks (483) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 483 pairs to build model! INFO @ Fri, 10 Dec 2021 14:27:47: start model_add_line... INFO @ Fri, 10 Dec 2021 14:27:47: start X-correlation... INFO @ Fri, 10 Dec 2021 14:27:47: end of X-cor INFO @ Fri, 10 Dec 2021 14:27:47: #2 finished! INFO @ Fri, 10 Dec 2021 14:27:47: #2 predicted fragment length is 195 bps INFO @ Fri, 10 Dec 2021 14:27:47: #2 alternative fragment length(s) may be 195 bps INFO @ Fri, 10 Dec 2021 14:27:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20_model.r WARNING @ Fri, 10 Dec 2021 14:27:47: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:27:47: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Fri, 10 Dec 2021 14:27:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:27:47: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:27:47: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:28:55: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:29:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20_peaks.xls INFO @ Fri, 10 Dec 2021 14:29:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:29:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694698/SRX8694698.20_summits.bed INFO @ Fri, 10 Dec 2021 14:29:25: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1351 records, 4 fields): 154 millis CompletedMACS2peakCalling