Job ID = 14167038 SRX = SRX8694695 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 44198489 spots for SRR12180113/SRR12180113.sra Written 44198489 spots for SRR12180113/SRR12180113.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167955 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:44:08 44198489 reads; of these: 44198489 (100.00%) were paired; of these: 13785931 (31.19%) aligned concordantly 0 times 21342012 (48.29%) aligned concordantly exactly 1 time 9070546 (20.52%) aligned concordantly >1 times ---- 13785931 pairs aligned concordantly 0 times; of these: 3860787 (28.01%) aligned discordantly 1 time ---- 9925144 pairs aligned 0 times concordantly or discordantly; of these: 19850288 mates make up the pairs; of these: 10859504 (54.71%) aligned 0 times 4119411 (20.75%) aligned exactly 1 time 4871373 (24.54%) aligned >1 times 87.72% overall alignment rate Time searching: 02:44:08 Overall time: 02:44:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3985092 / 32749930 = 0.1217 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:22:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:22:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:22:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:22:25: 1000000 INFO @ Fri, 10 Dec 2021 12:22:33: 2000000 INFO @ Fri, 10 Dec 2021 12:22:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:22:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:22:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:22:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:22:47: 4000000 INFO @ Fri, 10 Dec 2021 12:22:56: 5000000 INFO @ Fri, 10 Dec 2021 12:22:56: 1000000 INFO @ Fri, 10 Dec 2021 12:23:05: 6000000 INFO @ Fri, 10 Dec 2021 12:23:05: 2000000 INFO @ Fri, 10 Dec 2021 12:23:13: 7000000 INFO @ Fri, 10 Dec 2021 12:23:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:23:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:23:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:23:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:23:22: 8000000 INFO @ Fri, 10 Dec 2021 12:23:23: 4000000 INFO @ Fri, 10 Dec 2021 12:23:26: 1000000 INFO @ Fri, 10 Dec 2021 12:23:31: 9000000 INFO @ Fri, 10 Dec 2021 12:23:32: 5000000 INFO @ Fri, 10 Dec 2021 12:23:36: 2000000 INFO @ Fri, 10 Dec 2021 12:23:39: 10000000 INFO @ Fri, 10 Dec 2021 12:23:41: 6000000 INFO @ Fri, 10 Dec 2021 12:23:44: 3000000 INFO @ Fri, 10 Dec 2021 12:23:48: 11000000 INFO @ Fri, 10 Dec 2021 12:23:50: 7000000 INFO @ Fri, 10 Dec 2021 12:23:53: 4000000 INFO @ Fri, 10 Dec 2021 12:23:57: 12000000 INFO @ Fri, 10 Dec 2021 12:23:59: 8000000 INFO @ Fri, 10 Dec 2021 12:24:02: 5000000 INFO @ Fri, 10 Dec 2021 12:24:05: 13000000 INFO @ Fri, 10 Dec 2021 12:24:08: 9000000 INFO @ Fri, 10 Dec 2021 12:24:11: 6000000 INFO @ Fri, 10 Dec 2021 12:24:14: 14000000 INFO @ Fri, 10 Dec 2021 12:24:17: 10000000 INFO @ Fri, 10 Dec 2021 12:24:20: 7000000 INFO @ Fri, 10 Dec 2021 12:24:23: 15000000 INFO @ Fri, 10 Dec 2021 12:24:26: 11000000 INFO @ Fri, 10 Dec 2021 12:24:29: 8000000 INFO @ Fri, 10 Dec 2021 12:24:34: 16000000 INFO @ Fri, 10 Dec 2021 12:24:34: 12000000 INFO @ Fri, 10 Dec 2021 12:24:38: 9000000 INFO @ Fri, 10 Dec 2021 12:24:43: 13000000 INFO @ Fri, 10 Dec 2021 12:24:44: 17000000 INFO @ Fri, 10 Dec 2021 12:24:47: 10000000 INFO @ Fri, 10 Dec 2021 12:24:52: 14000000 INFO @ Fri, 10 Dec 2021 12:24:53: 18000000 INFO @ Fri, 10 Dec 2021 12:24:56: 11000000 INFO @ Fri, 10 Dec 2021 12:25:02: 15000000 INFO @ Fri, 10 Dec 2021 12:25:02: 19000000 INFO @ Fri, 10 Dec 2021 12:25:04: 12000000 INFO @ Fri, 10 Dec 2021 12:25:11: 20000000 INFO @ Fri, 10 Dec 2021 12:25:12: 16000000 INFO @ Fri, 10 Dec 2021 12:25:13: 13000000 INFO @ Fri, 10 Dec 2021 12:25:20: 21000000 INFO @ Fri, 10 Dec 2021 12:25:22: 14000000 INFO @ Fri, 10 Dec 2021 12:25:22: 17000000 INFO @ Fri, 10 Dec 2021 12:25:28: 22000000 INFO @ Fri, 10 Dec 2021 12:25:31: 15000000 INFO @ Fri, 10 Dec 2021 12:25:32: 18000000 INFO @ Fri, 10 Dec 2021 12:25:37: 23000000 INFO @ Fri, 10 Dec 2021 12:25:41: 19000000 INFO @ Fri, 10 Dec 2021 12:25:41: 16000000 INFO @ Fri, 10 Dec 2021 12:25:45: 24000000 INFO @ Fri, 10 Dec 2021 12:25:50: 20000000 INFO @ Fri, 10 Dec 2021 12:25:51: 17000000 INFO @ Fri, 10 Dec 2021 12:25:54: 25000000 INFO @ Fri, 10 Dec 2021 12:25:59: 21000000 INFO @ Fri, 10 Dec 2021 12:26:01: 18000000 INFO @ Fri, 10 Dec 2021 12:26:03: 26000000 INFO @ Fri, 10 Dec 2021 12:26:08: 22000000 INFO @ Fri, 10 Dec 2021 12:26:10: 19000000 INFO @ Fri, 10 Dec 2021 12:26:12: 27000000 INFO @ Fri, 10 Dec 2021 12:26:17: 23000000 INFO @ Fri, 10 Dec 2021 12:26:19: 20000000 INFO @ Fri, 10 Dec 2021 12:26:20: 28000000 INFO @ Fri, 10 Dec 2021 12:26:26: 24000000 INFO @ Fri, 10 Dec 2021 12:26:28: 21000000 INFO @ Fri, 10 Dec 2021 12:26:29: 29000000 INFO @ Fri, 10 Dec 2021 12:26:35: 25000000 INFO @ Fri, 10 Dec 2021 12:26:37: 22000000 INFO @ Fri, 10 Dec 2021 12:26:38: 30000000 INFO @ Fri, 10 Dec 2021 12:26:44: 26000000 INFO @ Fri, 10 Dec 2021 12:26:46: 23000000 INFO @ Fri, 10 Dec 2021 12:26:47: 31000000 INFO @ Fri, 10 Dec 2021 12:26:53: 27000000 INFO @ Fri, 10 Dec 2021 12:26:55: 24000000 INFO @ Fri, 10 Dec 2021 12:26:55: 32000000 INFO @ Fri, 10 Dec 2021 12:27:02: 28000000 INFO @ Fri, 10 Dec 2021 12:27:04: 25000000 INFO @ Fri, 10 Dec 2021 12:27:04: 33000000 INFO @ Fri, 10 Dec 2021 12:27:10: 29000000 INFO @ Fri, 10 Dec 2021 12:27:12: 26000000 INFO @ Fri, 10 Dec 2021 12:27:13: 34000000 INFO @ Fri, 10 Dec 2021 12:27:19: 30000000 INFO @ Fri, 10 Dec 2021 12:27:21: 27000000 INFO @ Fri, 10 Dec 2021 12:27:22: 35000000 INFO @ Fri, 10 Dec 2021 12:27:28: 31000000 INFO @ Fri, 10 Dec 2021 12:27:30: 28000000 INFO @ Fri, 10 Dec 2021 12:27:32: 36000000 INFO @ Fri, 10 Dec 2021 12:27:37: 32000000 INFO @ Fri, 10 Dec 2021 12:27:39: 29000000 INFO @ Fri, 10 Dec 2021 12:27:42: 37000000 INFO @ Fri, 10 Dec 2021 12:27:46: 33000000 INFO @ Fri, 10 Dec 2021 12:27:48: 30000000 INFO @ Fri, 10 Dec 2021 12:27:51: 38000000 INFO @ Fri, 10 Dec 2021 12:27:55: 34000000 INFO @ Fri, 10 Dec 2021 12:27:57: 31000000 INFO @ Fri, 10 Dec 2021 12:28:01: 39000000 INFO @ Fri, 10 Dec 2021 12:28:05: 35000000 INFO @ Fri, 10 Dec 2021 12:28:06: 32000000 INFO @ Fri, 10 Dec 2021 12:28:11: 40000000 INFO @ Fri, 10 Dec 2021 12:28:14: 33000000 INFO @ Fri, 10 Dec 2021 12:28:15: 36000000 INFO @ Fri, 10 Dec 2021 12:28:21: 41000000 INFO @ Fri, 10 Dec 2021 12:28:23: 34000000 INFO @ Fri, 10 Dec 2021 12:28:25: 37000000 INFO @ Fri, 10 Dec 2021 12:28:31: 42000000 INFO @ Fri, 10 Dec 2021 12:28:33: 35000000 INFO @ Fri, 10 Dec 2021 12:28:34: 38000000 INFO @ Fri, 10 Dec 2021 12:28:39: 43000000 INFO @ Fri, 10 Dec 2021 12:28:43: 36000000 INFO @ Fri, 10 Dec 2021 12:28:44: 39000000 INFO @ Fri, 10 Dec 2021 12:28:48: 44000000 INFO @ Fri, 10 Dec 2021 12:28:52: 37000000 INFO @ Fri, 10 Dec 2021 12:28:54: 40000000 INFO @ Fri, 10 Dec 2021 12:28:57: 45000000 INFO @ Fri, 10 Dec 2021 12:29:02: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:29:05: 41000000 INFO @ Fri, 10 Dec 2021 12:29:06: 46000000 INFO @ Fri, 10 Dec 2021 12:29:12: 39000000 INFO @ Fri, 10 Dec 2021 12:29:15: 47000000 INFO @ Fri, 10 Dec 2021 12:29:16: 42000000 INFO @ Fri, 10 Dec 2021 12:29:23: 40000000 INFO @ Fri, 10 Dec 2021 12:29:24: 48000000 INFO @ Fri, 10 Dec 2021 12:29:25: 43000000 INFO @ Fri, 10 Dec 2021 12:29:33: 49000000 INFO @ Fri, 10 Dec 2021 12:29:34: 41000000 INFO @ Fri, 10 Dec 2021 12:29:35: 44000000 INFO @ Fri, 10 Dec 2021 12:29:42: 50000000 INFO @ Fri, 10 Dec 2021 12:29:44: 45000000 INFO @ Fri, 10 Dec 2021 12:29:44: 42000000 INFO @ Fri, 10 Dec 2021 12:29:51: 51000000 INFO @ Fri, 10 Dec 2021 12:29:53: 46000000 INFO @ Fri, 10 Dec 2021 12:29:54: 43000000 INFO @ Fri, 10 Dec 2021 12:30:00: 52000000 INFO @ Fri, 10 Dec 2021 12:30:02: 47000000 INFO @ Fri, 10 Dec 2021 12:30:03: 44000000 INFO @ Fri, 10 Dec 2021 12:30:09: 53000000 INFO @ Fri, 10 Dec 2021 12:30:12: 48000000 INFO @ Fri, 10 Dec 2021 12:30:12: 45000000 INFO @ Fri, 10 Dec 2021 12:30:18: 54000000 INFO @ Fri, 10 Dec 2021 12:30:21: 49000000 INFO @ Fri, 10 Dec 2021 12:30:21: 46000000 INFO @ Fri, 10 Dec 2021 12:30:27: 55000000 INFO @ Fri, 10 Dec 2021 12:30:30: 50000000 INFO @ Fri, 10 Dec 2021 12:30:30: 47000000 INFO @ Fri, 10 Dec 2021 12:30:36: 56000000 INFO @ Fri, 10 Dec 2021 12:30:38: 51000000 INFO @ Fri, 10 Dec 2021 12:30:39: 48000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:30:45: 57000000 INFO @ Fri, 10 Dec 2021 12:30:47: 52000000 INFO @ Fri, 10 Dec 2021 12:30:48: 49000000 INFO @ Fri, 10 Dec 2021 12:30:54: 58000000 INFO @ Fri, 10 Dec 2021 12:30:56: 50000000 INFO @ Fri, 10 Dec 2021 12:30:56: 53000000 INFO @ Fri, 10 Dec 2021 12:31:03: 59000000 INFO @ Fri, 10 Dec 2021 12:31:05: 51000000 INFO @ Fri, 10 Dec 2021 12:31:05: 54000000 INFO @ Fri, 10 Dec 2021 12:31:12: 60000000 INFO @ Fri, 10 Dec 2021 12:31:14: 52000000 INFO @ Fri, 10 Dec 2021 12:31:15: 55000000 INFO @ Fri, 10 Dec 2021 12:31:21: 61000000 INFO @ Fri, 10 Dec 2021 12:31:23: 53000000 INFO @ Fri, 10 Dec 2021 12:31:24: 56000000 INFO @ Fri, 10 Dec 2021 12:31:30: 62000000 INFO @ Fri, 10 Dec 2021 12:31:32: 54000000 INFO @ Fri, 10 Dec 2021 12:31:33: 57000000 INFO @ Fri, 10 Dec 2021 12:31:39: 63000000 INFO @ Fri, 10 Dec 2021 12:31:41: 55000000 INFO @ Fri, 10 Dec 2021 12:31:42: 58000000 INFO @ Fri, 10 Dec 2021 12:31:48: 64000000 INFO @ Fri, 10 Dec 2021 12:31:50: 56000000 INFO @ Fri, 10 Dec 2021 12:31:51: 59000000 INFO @ Fri, 10 Dec 2021 12:31:57: 65000000 INFO @ Fri, 10 Dec 2021 12:31:59: 57000000 INFO @ Fri, 10 Dec 2021 12:32:00: 60000000 INFO @ Fri, 10 Dec 2021 12:32:06: 66000000 INFO @ Fri, 10 Dec 2021 12:32:08: 58000000 INFO @ Fri, 10 Dec 2021 12:32:09: 61000000 INFO @ Fri, 10 Dec 2021 12:32:15: 67000000 INFO @ Fri, 10 Dec 2021 12:32:17: 59000000 INFO @ Fri, 10 Dec 2021 12:32:18: 62000000 INFO @ Fri, 10 Dec 2021 12:32:24: 68000000 INFO @ Fri, 10 Dec 2021 12:32:26: 60000000 INFO @ Fri, 10 Dec 2021 12:32:28: 63000000 INFO @ Fri, 10 Dec 2021 12:32:34: 69000000 INFO @ Fri, 10 Dec 2021 12:32:35: 61000000 INFO @ Fri, 10 Dec 2021 12:32:37: 64000000 INFO @ Fri, 10 Dec 2021 12:32:39: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:32:39: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:32:39: #1 total tags in treatment: 26666411 INFO @ Fri, 10 Dec 2021 12:32:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:32:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:32:39: #1 tags after filtering in treatment: 23589830 INFO @ Fri, 10 Dec 2021 12:32:39: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 10 Dec 2021 12:32:39: #1 finished! INFO @ Fri, 10 Dec 2021 12:32:39: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:32:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:32:41: #2 number of paired peaks: 504 WARNING @ Fri, 10 Dec 2021 12:32:41: Fewer paired peaks (504) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 504 pairs to build model! INFO @ Fri, 10 Dec 2021 12:32:41: start model_add_line... INFO @ Fri, 10 Dec 2021 12:32:41: start X-correlation... INFO @ Fri, 10 Dec 2021 12:32:41: end of X-cor INFO @ Fri, 10 Dec 2021 12:32:41: #2 finished! INFO @ Fri, 10 Dec 2021 12:32:41: #2 predicted fragment length is 198 bps INFO @ Fri, 10 Dec 2021 12:32:41: #2 alternative fragment length(s) may be 198 bps INFO @ Fri, 10 Dec 2021 12:32:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05_model.r WARNING @ Fri, 10 Dec 2021 12:32:41: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:32:41: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Fri, 10 Dec 2021 12:32:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:32:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:32:41: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:32:44: 62000000 INFO @ Fri, 10 Dec 2021 12:32:46: 65000000 INFO @ Fri, 10 Dec 2021 12:32:53: 63000000 INFO @ Fri, 10 Dec 2021 12:32:55: 66000000 INFO @ Fri, 10 Dec 2021 12:33:02: 64000000 INFO @ Fri, 10 Dec 2021 12:33:04: 67000000 INFO @ Fri, 10 Dec 2021 12:33:11: 65000000 INFO @ Fri, 10 Dec 2021 12:33:13: 68000000 INFO @ Fri, 10 Dec 2021 12:33:20: 66000000 INFO @ Fri, 10 Dec 2021 12:33:22: 69000000 INFO @ Fri, 10 Dec 2021 12:33:25: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:33:27: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:33:27: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:33:27: #1 total tags in treatment: 26666411 INFO @ Fri, 10 Dec 2021 12:33:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:33:28: #1 tags after filtering in treatment: 23589830 INFO @ Fri, 10 Dec 2021 12:33:28: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 10 Dec 2021 12:33:28: #1 finished! INFO @ Fri, 10 Dec 2021 12:33:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:33:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:33:29: 67000000 INFO @ Fri, 10 Dec 2021 12:33:29: #2 number of paired peaks: 504 WARNING @ Fri, 10 Dec 2021 12:33:29: Fewer paired peaks (504) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 504 pairs to build model! INFO @ Fri, 10 Dec 2021 12:33:29: start model_add_line... INFO @ Fri, 10 Dec 2021 12:33:29: start X-correlation... INFO @ Fri, 10 Dec 2021 12:33:29: end of X-cor INFO @ Fri, 10 Dec 2021 12:33:29: #2 finished! INFO @ Fri, 10 Dec 2021 12:33:29: #2 predicted fragment length is 198 bps INFO @ Fri, 10 Dec 2021 12:33:29: #2 alternative fragment length(s) may be 198 bps INFO @ Fri, 10 Dec 2021 12:33:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10_model.r WARNING @ Fri, 10 Dec 2021 12:33:29: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:33:29: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Fri, 10 Dec 2021 12:33:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:33:29: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:33:29: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:33:37: 68000000 INFO @ Fri, 10 Dec 2021 12:33:46: 69000000 INFO @ Fri, 10 Dec 2021 12:33:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05_peaks.xls INFO @ Fri, 10 Dec 2021 12:33:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:33:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.05_summits.bed INFO @ Fri, 10 Dec 2021 12:33:46: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3555 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:33:50: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:33:50: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:33:50: #1 total tags in treatment: 26666411 INFO @ Fri, 10 Dec 2021 12:33:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:33:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:33:51: #1 tags after filtering in treatment: 23589830 INFO @ Fri, 10 Dec 2021 12:33:51: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 10 Dec 2021 12:33:51: #1 finished! INFO @ Fri, 10 Dec 2021 12:33:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:33:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:33:52: #2 number of paired peaks: 504 WARNING @ Fri, 10 Dec 2021 12:33:52: Fewer paired peaks (504) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 504 pairs to build model! INFO @ Fri, 10 Dec 2021 12:33:52: start model_add_line... INFO @ Fri, 10 Dec 2021 12:33:52: start X-correlation... INFO @ Fri, 10 Dec 2021 12:33:52: end of X-cor INFO @ Fri, 10 Dec 2021 12:33:52: #2 finished! INFO @ Fri, 10 Dec 2021 12:33:52: #2 predicted fragment length is 198 bps INFO @ Fri, 10 Dec 2021 12:33:52: #2 alternative fragment length(s) may be 198 bps INFO @ Fri, 10 Dec 2021 12:33:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20_model.r WARNING @ Fri, 10 Dec 2021 12:33:52: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:33:52: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Fri, 10 Dec 2021 12:33:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:33:52: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:33:52: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:34:14: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:34:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10_peaks.xls INFO @ Fri, 10 Dec 2021 12:34:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:34:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.10_summits.bed INFO @ Fri, 10 Dec 2021 12:34:35: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2336 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:34:37: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:34:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20_peaks.xls INFO @ Fri, 10 Dec 2021 12:34:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:34:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694695/SRX8694695.20_summits.bed INFO @ Fri, 10 Dec 2021 12:34:58: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (1341 records, 4 fields): 3 millis CompletedMACS2peakCalling