Job ID = 14170336 SRX = SRX8689107 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 29448952 spots for SRR12174340/SRR12174340.sra Written 29448952 spots for SRR12174340/SRR12174340.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170782 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:01 29448952 reads; of these: 29448952 (100.00%) were unpaired; of these: 676355 (2.30%) aligned 0 times 19769453 (67.13%) aligned exactly 1 time 9003144 (30.57%) aligned >1 times 97.70% overall alignment rate Time searching: 00:14:01 Overall time: 00:14:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3602109 / 28772597 = 0.1252 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:45:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:45:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:45:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:45:20: 1000000 INFO @ Sat, 11 Dec 2021 06:45:29: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:45:39: 3000000 INFO @ Sat, 11 Dec 2021 06:45:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:45:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:45:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:45:48: 4000000 INFO @ Sat, 11 Dec 2021 06:45:49: 1000000 INFO @ Sat, 11 Dec 2021 06:45:58: 2000000 INFO @ Sat, 11 Dec 2021 06:45:58: 5000000 INFO @ Sat, 11 Dec 2021 06:46:07: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:46:08: 6000000 INFO @ Sat, 11 Dec 2021 06:46:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:46:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:46:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:46:16: 4000000 INFO @ Sat, 11 Dec 2021 06:46:18: 7000000 INFO @ Sat, 11 Dec 2021 06:46:23: 1000000 INFO @ Sat, 11 Dec 2021 06:46:25: 5000000 INFO @ Sat, 11 Dec 2021 06:46:27: 8000000 INFO @ Sat, 11 Dec 2021 06:46:34: 6000000 INFO @ Sat, 11 Dec 2021 06:46:36: 2000000 INFO @ Sat, 11 Dec 2021 06:46:36: 9000000 INFO @ Sat, 11 Dec 2021 06:46:45: 7000000 INFO @ Sat, 11 Dec 2021 06:46:45: 10000000 INFO @ Sat, 11 Dec 2021 06:46:48: 3000000 INFO @ Sat, 11 Dec 2021 06:46:54: 11000000 INFO @ Sat, 11 Dec 2021 06:46:55: 8000000 INFO @ Sat, 11 Dec 2021 06:47:00: 4000000 INFO @ Sat, 11 Dec 2021 06:47:02: 12000000 INFO @ Sat, 11 Dec 2021 06:47:05: 9000000 INFO @ Sat, 11 Dec 2021 06:47:11: 13000000 INFO @ Sat, 11 Dec 2021 06:47:12: 5000000 INFO @ Sat, 11 Dec 2021 06:47:15: 10000000 INFO @ Sat, 11 Dec 2021 06:47:19: 14000000 INFO @ Sat, 11 Dec 2021 06:47:23: 11000000 INFO @ Sat, 11 Dec 2021 06:47:24: 6000000 INFO @ Sat, 11 Dec 2021 06:47:27: 15000000 INFO @ Sat, 11 Dec 2021 06:47:32: 12000000 INFO @ Sat, 11 Dec 2021 06:47:35: 7000000 INFO @ Sat, 11 Dec 2021 06:47:36: 16000000 INFO @ Sat, 11 Dec 2021 06:47:40: 13000000 INFO @ Sat, 11 Dec 2021 06:47:44: 17000000 INFO @ Sat, 11 Dec 2021 06:47:47: 8000000 INFO @ Sat, 11 Dec 2021 06:47:49: 14000000 INFO @ Sat, 11 Dec 2021 06:47:53: 18000000 INFO @ Sat, 11 Dec 2021 06:47:57: 15000000 INFO @ Sat, 11 Dec 2021 06:47:59: 9000000 INFO @ Sat, 11 Dec 2021 06:48:01: 19000000 INFO @ Sat, 11 Dec 2021 06:48:06: 16000000 INFO @ Sat, 11 Dec 2021 06:48:09: 20000000 INFO @ Sat, 11 Dec 2021 06:48:11: 10000000 INFO @ Sat, 11 Dec 2021 06:48:15: 17000000 INFO @ Sat, 11 Dec 2021 06:48:18: 21000000 INFO @ Sat, 11 Dec 2021 06:48:22: 11000000 INFO @ Sat, 11 Dec 2021 06:48:23: 18000000 INFO @ Sat, 11 Dec 2021 06:48:27: 22000000 INFO @ Sat, 11 Dec 2021 06:48:32: 19000000 INFO @ Sat, 11 Dec 2021 06:48:34: 12000000 INFO @ Sat, 11 Dec 2021 06:48:36: 23000000 INFO @ Sat, 11 Dec 2021 06:48:41: 20000000 INFO @ Sat, 11 Dec 2021 06:48:45: 24000000 INFO @ Sat, 11 Dec 2021 06:48:45: 13000000 INFO @ Sat, 11 Dec 2021 06:48:50: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:48:53: 25000000 INFO @ Sat, 11 Dec 2021 06:48:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:48:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:48:55: #1 total tags in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:48:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:48:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:48:55: #1 tags after filtering in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:48:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:48:55: #1 finished! INFO @ Sat, 11 Dec 2021 06:48:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:48:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:48:57: 14000000 INFO @ Sat, 11 Dec 2021 06:48:57: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 06:48:57: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:48:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:48:58: 22000000 INFO @ Sat, 11 Dec 2021 06:49:06: 23000000 INFO @ Sat, 11 Dec 2021 06:49:08: 15000000 INFO @ Sat, 11 Dec 2021 06:49:15: 24000000 INFO @ Sat, 11 Dec 2021 06:49:19: 16000000 INFO @ Sat, 11 Dec 2021 06:49:24: 25000000 INFO @ Sat, 11 Dec 2021 06:49:25: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:49:25: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:49:25: #1 total tags in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:49:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:49:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:49:26: #1 tags after filtering in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:49:26: #1 finished! INFO @ Sat, 11 Dec 2021 06:49:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:49:28: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 06:49:28: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:49:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:49:30: 17000000 INFO @ Sat, 11 Dec 2021 06:49:41: 18000000 INFO @ Sat, 11 Dec 2021 06:49:52: 19000000 INFO @ Sat, 11 Dec 2021 06:50:04: 20000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:50:16: 21000000 INFO @ Sat, 11 Dec 2021 06:50:28: 22000000 INFO @ Sat, 11 Dec 2021 06:50:40: 23000000 INFO @ Sat, 11 Dec 2021 06:50:52: 24000000 INFO @ Sat, 11 Dec 2021 06:51:04: 25000000 INFO @ Sat, 11 Dec 2021 06:51:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:51:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:51:06: #1 total tags in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:51:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:51:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:51:07: #1 tags after filtering in treatment: 25170488 INFO @ Sat, 11 Dec 2021 06:51:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:51:07: #1 finished! INFO @ Sat, 11 Dec 2021 06:51:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:51:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:51:09: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 06:51:09: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:51:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689107/SRX8689107.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling