Job ID = 14170321 SRX = SRX8689103 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 29136872 spots for SRR12174336/SRR12174336.sra Written 29136872 spots for SRR12174336/SRR12174336.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170760 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:48 29136872 reads; of these: 29136872 (100.00%) were unpaired; of these: 811956 (2.79%) aligned 0 times 20408927 (70.05%) aligned exactly 1 time 7915989 (27.17%) aligned >1 times 97.21% overall alignment rate Time searching: 00:09:48 Overall time: 00:09:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3180844 / 28324916 = 0.1123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:35:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:35:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:35:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:35:53: 1000000 INFO @ Sat, 11 Dec 2021 06:35:59: 2000000 INFO @ Sat, 11 Dec 2021 06:36:04: 3000000 INFO @ Sat, 11 Dec 2021 06:36:09: 4000000 INFO @ Sat, 11 Dec 2021 06:36:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:36:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:36:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:36:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:36:20: 6000000 INFO @ Sat, 11 Dec 2021 06:36:24: 1000000 INFO @ Sat, 11 Dec 2021 06:36:26: 7000000 INFO @ Sat, 11 Dec 2021 06:36:29: 2000000 INFO @ Sat, 11 Dec 2021 06:36:31: 8000000 INFO @ Sat, 11 Dec 2021 06:36:35: 3000000 INFO @ Sat, 11 Dec 2021 06:36:36: 9000000 INFO @ Sat, 11 Dec 2021 06:36:40: 4000000 INFO @ Sat, 11 Dec 2021 06:36:42: 10000000 INFO @ Sat, 11 Dec 2021 06:36:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:36:47: 11000000 INFO @ Sat, 11 Dec 2021 06:36:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:36:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:36:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:36:51: 6000000 INFO @ Sat, 11 Dec 2021 06:36:52: 12000000 INFO @ Sat, 11 Dec 2021 06:36:53: 1000000 INFO @ Sat, 11 Dec 2021 06:36:57: 7000000 INFO @ Sat, 11 Dec 2021 06:36:57: 2000000 INFO @ Sat, 11 Dec 2021 06:36:57: 13000000 INFO @ Sat, 11 Dec 2021 06:37:02: 8000000 INFO @ Sat, 11 Dec 2021 06:37:02: 3000000 INFO @ Sat, 11 Dec 2021 06:37:03: 14000000 INFO @ Sat, 11 Dec 2021 06:37:07: 4000000 INFO @ Sat, 11 Dec 2021 06:37:08: 9000000 INFO @ Sat, 11 Dec 2021 06:37:08: 15000000 INFO @ Sat, 11 Dec 2021 06:37:12: 5000000 INFO @ Sat, 11 Dec 2021 06:37:13: 16000000 INFO @ Sat, 11 Dec 2021 06:37:13: 10000000 INFO @ Sat, 11 Dec 2021 06:37:16: 6000000 INFO @ Sat, 11 Dec 2021 06:37:18: 11000000 INFO @ Sat, 11 Dec 2021 06:37:18: 17000000 INFO @ Sat, 11 Dec 2021 06:37:21: 7000000 INFO @ Sat, 11 Dec 2021 06:37:23: 12000000 INFO @ Sat, 11 Dec 2021 06:37:23: 18000000 INFO @ Sat, 11 Dec 2021 06:37:26: 8000000 INFO @ Sat, 11 Dec 2021 06:37:29: 13000000 INFO @ Sat, 11 Dec 2021 06:37:29: 19000000 INFO @ Sat, 11 Dec 2021 06:37:30: 9000000 INFO @ Sat, 11 Dec 2021 06:37:34: 14000000 INFO @ Sat, 11 Dec 2021 06:37:34: 20000000 INFO @ Sat, 11 Dec 2021 06:37:35: 10000000 INFO @ Sat, 11 Dec 2021 06:37:39: 15000000 INFO @ Sat, 11 Dec 2021 06:37:39: 21000000 INFO @ Sat, 11 Dec 2021 06:37:40: 11000000 INFO @ Sat, 11 Dec 2021 06:37:44: 12000000 INFO @ Sat, 11 Dec 2021 06:37:44: 16000000 INFO @ Sat, 11 Dec 2021 06:37:44: 22000000 INFO @ Sat, 11 Dec 2021 06:37:49: 13000000 INFO @ Sat, 11 Dec 2021 06:37:49: 23000000 INFO @ Sat, 11 Dec 2021 06:37:50: 17000000 INFO @ Sat, 11 Dec 2021 06:37:53: 14000000 INFO @ Sat, 11 Dec 2021 06:37:55: 24000000 INFO @ Sat, 11 Dec 2021 06:37:55: 18000000 INFO @ Sat, 11 Dec 2021 06:37:58: 15000000 INFO @ Sat, 11 Dec 2021 06:38:00: 25000000 INFO @ Sat, 11 Dec 2021 06:38:00: 19000000 INFO @ Sat, 11 Dec 2021 06:38:01: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:38:01: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:38:01: #1 total tags in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:38:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:38:01: #1 tags after filtering in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:38:01: #1 finished! INFO @ Sat, 11 Dec 2021 06:38:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:38:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:38:03: 16000000 INFO @ Sat, 11 Dec 2021 06:38:03: #2 number of paired peaks: 42 WARNING @ Sat, 11 Dec 2021 06:38:03: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:38:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:38:05: 20000000 INFO @ Sat, 11 Dec 2021 06:38:07: 17000000 INFO @ Sat, 11 Dec 2021 06:38:10: 21000000 INFO @ Sat, 11 Dec 2021 06:38:12: 18000000 INFO @ Sat, 11 Dec 2021 06:38:16: 22000000 INFO @ Sat, 11 Dec 2021 06:38:16: 19000000 INFO @ Sat, 11 Dec 2021 06:38:21: 23000000 INFO @ Sat, 11 Dec 2021 06:38:21: 20000000 INFO @ Sat, 11 Dec 2021 06:38:25: 21000000 INFO @ Sat, 11 Dec 2021 06:38:26: 24000000 INFO @ Sat, 11 Dec 2021 06:38:30: 22000000 INFO @ Sat, 11 Dec 2021 06:38:31: 25000000 INFO @ Sat, 11 Dec 2021 06:38:32: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:38:32: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:38:32: #1 total tags in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:38:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:38:32: #1 tags after filtering in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:38:32: #1 finished! INFO @ Sat, 11 Dec 2021 06:38:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:38:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:38:34: #2 number of paired peaks: 42 WARNING @ Sat, 11 Dec 2021 06:38:34: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:38:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:38:34: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:38:39: 24000000 INFO @ Sat, 11 Dec 2021 06:38:43: 25000000 INFO @ Sat, 11 Dec 2021 06:38:44: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:38:44: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:38:44: #1 total tags in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:38:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:38:45: #1 tags after filtering in treatment: 25144072 INFO @ Sat, 11 Dec 2021 06:38:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:38:45: #1 finished! INFO @ Sat, 11 Dec 2021 06:38:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:38:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:38:46: #2 number of paired peaks: 42 WARNING @ Sat, 11 Dec 2021 06:38:46: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:38:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689103/SRX8689103.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。