Job ID = 14170317 SRX = SRX8689100 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6445005 spots for SRR12174358/SRR12174358.sra Written 6445005 spots for SRR12174358/SRR12174358.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170737 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:44 6445005 reads; of these: 6445005 (100.00%) were unpaired; of these: 300158 (4.66%) aligned 0 times 4993051 (77.47%) aligned exactly 1 time 1151796 (17.87%) aligned >1 times 95.34% overall alignment rate Time searching: 00:01:45 Overall time: 00:01:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 213029 / 6144847 = 0.0347 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:19:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:19:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:19:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:19:50: 1000000 INFO @ Sat, 11 Dec 2021 06:19:56: 2000000 INFO @ Sat, 11 Dec 2021 06:20:02: 3000000 INFO @ Sat, 11 Dec 2021 06:20:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:20:13: 5000000 INFO @ Sat, 11 Dec 2021 06:20:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:20:14: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:20:14: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:20:19: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:20:19: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:20:19: #1 total tags in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:20:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:20:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:20:20: #1 tags after filtering in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:20:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:20:20: #1 finished! INFO @ Sat, 11 Dec 2021 06:20:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:20:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:20:20: #2 number of paired peaks: 145 WARNING @ Sat, 11 Dec 2021 06:20:20: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Sat, 11 Dec 2021 06:20:20: start model_add_line... INFO @ Sat, 11 Dec 2021 06:20:20: start X-correlation... INFO @ Sat, 11 Dec 2021 06:20:20: end of X-cor INFO @ Sat, 11 Dec 2021 06:20:20: #2 finished! INFO @ Sat, 11 Dec 2021 06:20:20: #2 predicted fragment length is 50 bps INFO @ Sat, 11 Dec 2021 06:20:20: #2 alternative fragment length(s) may be 50 bps INFO @ Sat, 11 Dec 2021 06:20:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05_model.r WARNING @ Sat, 11 Dec 2021 06:20:20: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:20:20: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Sat, 11 Dec 2021 06:20:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:20:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:20:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:20:21: 1000000 INFO @ Sat, 11 Dec 2021 06:20:27: 2000000 INFO @ Sat, 11 Dec 2021 06:20:32: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:20:33: 3000000 INFO @ Sat, 11 Dec 2021 06:20:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05_peaks.xls INFO @ Sat, 11 Dec 2021 06:20:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:20:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.05_summits.bed INFO @ Sat, 11 Dec 2021 06:20:38: Done! pass1 - making usageList (11 chroms): 0 millis pass2 - checking and writing primary data (1103 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:20:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:20:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:20:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:20:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:20:46: 5000000 INFO @ Sat, 11 Dec 2021 06:20:51: 1000000 INFO @ Sat, 11 Dec 2021 06:20:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:20:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:20:52: #1 total tags in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:20:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:20:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:20:52: #1 tags after filtering in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:20:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:20:52: #1 finished! INFO @ Sat, 11 Dec 2021 06:20:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:20:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:20:53: #2 number of paired peaks: 145 WARNING @ Sat, 11 Dec 2021 06:20:53: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Sat, 11 Dec 2021 06:20:53: start model_add_line... INFO @ Sat, 11 Dec 2021 06:20:53: start X-correlation... INFO @ Sat, 11 Dec 2021 06:20:53: end of X-cor INFO @ Sat, 11 Dec 2021 06:20:53: #2 finished! INFO @ Sat, 11 Dec 2021 06:20:53: #2 predicted fragment length is 50 bps INFO @ Sat, 11 Dec 2021 06:20:53: #2 alternative fragment length(s) may be 50 bps INFO @ Sat, 11 Dec 2021 06:20:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10_model.r WARNING @ Sat, 11 Dec 2021 06:20:53: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:20:53: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Sat, 11 Dec 2021 06:20:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:20:53: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:20:53: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:20:57: 2000000 INFO @ Sat, 11 Dec 2021 06:21:04: 3000000 INFO @ Sat, 11 Dec 2021 06:21:05: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:21:10: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:21:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10_peaks.xls INFO @ Sat, 11 Dec 2021 06:21:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:21:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.10_summits.bed INFO @ Sat, 11 Dec 2021 06:21:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (823 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:21:16: 5000000 INFO @ Sat, 11 Dec 2021 06:21:22: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:21:22: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:21:22: #1 total tags in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:21:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:21:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:21:22: #1 tags after filtering in treatment: 5931818 INFO @ Sat, 11 Dec 2021 06:21:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:21:22: #1 finished! INFO @ Sat, 11 Dec 2021 06:21:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:21:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:21:22: #2 number of paired peaks: 145 WARNING @ Sat, 11 Dec 2021 06:21:22: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Sat, 11 Dec 2021 06:21:22: start model_add_line... INFO @ Sat, 11 Dec 2021 06:21:22: start X-correlation... INFO @ Sat, 11 Dec 2021 06:21:22: end of X-cor INFO @ Sat, 11 Dec 2021 06:21:22: #2 finished! INFO @ Sat, 11 Dec 2021 06:21:22: #2 predicted fragment length is 50 bps INFO @ Sat, 11 Dec 2021 06:21:22: #2 alternative fragment length(s) may be 50 bps INFO @ Sat, 11 Dec 2021 06:21:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20_model.r WARNING @ Sat, 11 Dec 2021 06:21:22: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:21:22: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Sat, 11 Dec 2021 06:21:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:21:22: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:21:22: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:21:34: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:21:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20_peaks.xls INFO @ Sat, 11 Dec 2021 06:21:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:21:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8689100/SRX8689100.20_summits.bed INFO @ Sat, 11 Dec 2021 06:21:40: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (466 records, 4 fields): 2 millis CompletedMACS2peakCalling