Job ID = 14170563 SRX = SRX8689094 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5034502 spots for SRR12174352/SRR12174352.sra Written 5034502 spots for SRR12174352/SRR12174352.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170957 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:28 5034502 reads; of these: 5034502 (100.00%) were unpaired; of these: 145273 (2.89%) aligned 0 times 3991016 (79.27%) aligned exactly 1 time 898213 (17.84%) aligned >1 times 97.11% overall alignment rate Time searching: 00:01:29 Overall time: 00:01:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 157771 / 4889229 = 0.0323 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:18:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:18:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:18:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:18:26: 1000000 INFO @ Sat, 11 Dec 2021 07:18:32: 2000000 INFO @ Sat, 11 Dec 2021 07:18:38: 3000000 INFO @ Sat, 11 Dec 2021 07:18:43: 4000000 INFO @ Sat, 11 Dec 2021 07:18:47: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:18:47: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:18:47: #1 total tags in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:18:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:18:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:18:47: #1 tags after filtering in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:18:47: #1 finished! INFO @ Sat, 11 Dec 2021 07:18:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:18:48: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 07:18:48: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:18:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:18:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:18:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:18:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:18:56: 1000000 INFO @ Sat, 11 Dec 2021 07:19:01: 2000000 INFO @ Sat, 11 Dec 2021 07:19:07: 3000000 INFO @ Sat, 11 Dec 2021 07:19:12: 4000000 INFO @ Sat, 11 Dec 2021 07:19:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:19:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:19:16: #1 total tags in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:19:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:19:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:19:17: #1 tags after filtering in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:19:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:19:17: #1 finished! INFO @ Sat, 11 Dec 2021 07:19:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:19:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:19:17: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 07:19:17: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:19:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:19:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:19:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:19:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:19:26: 1000000 INFO @ Sat, 11 Dec 2021 07:19:31: 2000000 INFO @ Sat, 11 Dec 2021 07:19:37: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:19:43: 4000000 INFO @ Sat, 11 Dec 2021 07:19:47: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:19:47: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:19:47: #1 total tags in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:19:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:19:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:19:47: #1 tags after filtering in treatment: 4731458 INFO @ Sat, 11 Dec 2021 07:19:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:19:47: #1 finished! INFO @ Sat, 11 Dec 2021 07:19:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:19:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:19:47: #2 number of paired peaks: 73 WARNING @ Sat, 11 Dec 2021 07:19:47: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:19:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689094/SRX8689094.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。