Job ID = 14170562 SRX = SRX8689093 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24997723 spots for SRR12174285/SRR12174285.sra Written 24997723 spots for SRR12174285/SRR12174285.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170991 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:35 24997723 reads; of these: 24997723 (100.00%) were unpaired; of these: 1020476 (4.08%) aligned 0 times 16862399 (67.46%) aligned exactly 1 time 7114848 (28.46%) aligned >1 times 95.92% overall alignment rate Time searching: 00:09:35 Overall time: 00:09:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2698109 / 23977247 = 0.1125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:31:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:31:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:31:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:31:23: 1000000 INFO @ Sat, 11 Dec 2021 07:31:28: 2000000 INFO @ Sat, 11 Dec 2021 07:31:34: 3000000 INFO @ Sat, 11 Dec 2021 07:31:39: 4000000 INFO @ Sat, 11 Dec 2021 07:31:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:31:47: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:31:47: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:31:49: 6000000 INFO @ Sat, 11 Dec 2021 07:31:54: 1000000 INFO @ Sat, 11 Dec 2021 07:31:56: 7000000 INFO @ Sat, 11 Dec 2021 07:32:01: 2000000 INFO @ Sat, 11 Dec 2021 07:32:02: 8000000 INFO @ Sat, 11 Dec 2021 07:32:08: 9000000 INFO @ Sat, 11 Dec 2021 07:32:08: 3000000 INFO @ Sat, 11 Dec 2021 07:32:14: 10000000 INFO @ Sat, 11 Dec 2021 07:32:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:32:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:32:17: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:32:17: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:32:20: 11000000 INFO @ Sat, 11 Dec 2021 07:32:22: 5000000 INFO @ Sat, 11 Dec 2021 07:32:25: 1000000 INFO @ Sat, 11 Dec 2021 07:32:26: 12000000 INFO @ Sat, 11 Dec 2021 07:32:29: 6000000 INFO @ Sat, 11 Dec 2021 07:32:32: 2000000 INFO @ Sat, 11 Dec 2021 07:32:32: 13000000 INFO @ Sat, 11 Dec 2021 07:32:36: 7000000 INFO @ Sat, 11 Dec 2021 07:32:38: 14000000 INFO @ Sat, 11 Dec 2021 07:32:39: 3000000 INFO @ Sat, 11 Dec 2021 07:32:43: 8000000 INFO @ Sat, 11 Dec 2021 07:32:45: 15000000 INFO @ Sat, 11 Dec 2021 07:32:46: 4000000 INFO @ Sat, 11 Dec 2021 07:32:50: 9000000 INFO @ Sat, 11 Dec 2021 07:32:51: 16000000 INFO @ Sat, 11 Dec 2021 07:32:53: 5000000 INFO @ Sat, 11 Dec 2021 07:32:57: 10000000 INFO @ Sat, 11 Dec 2021 07:32:57: 17000000 INFO @ Sat, 11 Dec 2021 07:33:00: 6000000 INFO @ Sat, 11 Dec 2021 07:33:03: 18000000 INFO @ Sat, 11 Dec 2021 07:33:04: 11000000 INFO @ Sat, 11 Dec 2021 07:33:07: 7000000 INFO @ Sat, 11 Dec 2021 07:33:10: 19000000 INFO @ Sat, 11 Dec 2021 07:33:11: 12000000 INFO @ Sat, 11 Dec 2021 07:33:13: 8000000 INFO @ Sat, 11 Dec 2021 07:33:16: 20000000 INFO @ Sat, 11 Dec 2021 07:33:18: 13000000 INFO @ Sat, 11 Dec 2021 07:33:20: 9000000 INFO @ Sat, 11 Dec 2021 07:33:22: 21000000 INFO @ Sat, 11 Dec 2021 07:33:24: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:33:24: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:33:24: #1 total tags in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:33:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:33:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:33:24: #1 tags after filtering in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:33:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:33:24: #1 finished! INFO @ Sat, 11 Dec 2021 07:33:24: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:33:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:33:25: 14000000 INFO @ Sat, 11 Dec 2021 07:33:26: #2 number of paired peaks: 99 WARNING @ Sat, 11 Dec 2021 07:33:26: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:33:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:33:27: 10000000 INFO @ Sat, 11 Dec 2021 07:33:32: 15000000 INFO @ Sat, 11 Dec 2021 07:33:34: 11000000 INFO @ Sat, 11 Dec 2021 07:33:39: 16000000 INFO @ Sat, 11 Dec 2021 07:33:41: 12000000 INFO @ Sat, 11 Dec 2021 07:33:46: 17000000 INFO @ Sat, 11 Dec 2021 07:33:48: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:33:53: 18000000 INFO @ Sat, 11 Dec 2021 07:33:55: 14000000 INFO @ Sat, 11 Dec 2021 07:34:00: 19000000 INFO @ Sat, 11 Dec 2021 07:34:02: 15000000 INFO @ Sat, 11 Dec 2021 07:34:07: 20000000 INFO @ Sat, 11 Dec 2021 07:34:09: 16000000 INFO @ Sat, 11 Dec 2021 07:34:14: 21000000 INFO @ Sat, 11 Dec 2021 07:34:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:34:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:34:16: #1 total tags in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:34:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:34:16: #1 tags after filtering in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:34:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:34:16: #1 finished! INFO @ Sat, 11 Dec 2021 07:34:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:34:16: 17000000 INFO @ Sat, 11 Dec 2021 07:34:17: #2 number of paired peaks: 99 WARNING @ Sat, 11 Dec 2021 07:34:17: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:34:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:34:23: 18000000 INFO @ Sat, 11 Dec 2021 07:34:30: 19000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:34:36: 20000000 INFO @ Sat, 11 Dec 2021 07:34:43: 21000000 INFO @ Sat, 11 Dec 2021 07:34:44: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:34:44: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:34:44: #1 total tags in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:34:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:34:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:34:45: #1 tags after filtering in treatment: 21279138 INFO @ Sat, 11 Dec 2021 07:34:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:34:45: #1 finished! INFO @ Sat, 11 Dec 2021 07:34:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:34:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:34:46: #2 number of paired peaks: 99 WARNING @ Sat, 11 Dec 2021 07:34:46: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689093/SRX8689093.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling