Job ID = 14170504 SRX = SRX8689063 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6567740 spots for SRR12174198/SRR12174198.sra Written 6567740 spots for SRR12174198/SRR12174198.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170903 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:04 6567740 reads; of these: 6567740 (100.00%) were unpaired; of these: 678118 (10.32%) aligned 0 times 4978956 (75.81%) aligned exactly 1 time 910666 (13.87%) aligned >1 times 89.68% overall alignment rate Time searching: 00:02:04 Overall time: 00:02:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 229400 / 5889622 = 0.0389 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:02:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:02:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:02:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:03:01: 1000000 INFO @ Sat, 11 Dec 2021 07:03:10: 2000000 INFO @ Sat, 11 Dec 2021 07:03:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:03:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:03:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:03:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:03:28: 4000000 INFO @ Sat, 11 Dec 2021 07:03:30: 1000000 INFO @ Sat, 11 Dec 2021 07:03:37: 5000000 INFO @ Sat, 11 Dec 2021 07:03:38: 2000000 INFO @ Sat, 11 Dec 2021 07:03:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:03:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:03:43: #1 total tags in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:03:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:03:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:03:43: #1 tags after filtering in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:03:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:03:43: #1 finished! INFO @ Sat, 11 Dec 2021 07:03:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:03:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:03:44: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 07:03:44: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:03:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:03:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:03:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:03:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:03:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:03:55: 4000000 INFO @ Sat, 11 Dec 2021 07:04:01: 1000000 INFO @ Sat, 11 Dec 2021 07:04:03: 5000000 INFO @ Sat, 11 Dec 2021 07:04:08: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:04:08: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:04:08: #1 total tags in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:04:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:04:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:04:08: #1 tags after filtering in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:04:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:04:08: #1 finished! INFO @ Sat, 11 Dec 2021 07:04:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:04:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:04:09: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 07:04:09: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:04:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:04:10: 2000000 INFO @ Sat, 11 Dec 2021 07:04:19: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:04:28: 4000000 INFO @ Sat, 11 Dec 2021 07:04:37: 5000000 INFO @ Sat, 11 Dec 2021 07:04:42: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:04:42: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:04:42: #1 total tags in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:04:42: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:04:43: #1 tags after filtering in treatment: 5660222 INFO @ Sat, 11 Dec 2021 07:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:04:43: #1 finished! INFO @ Sat, 11 Dec 2021 07:04:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:04:43: #2 number of paired peaks: 30 WARNING @ Sat, 11 Dec 2021 07:04:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:04:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689063/SRX8689063.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。