Job ID = 14170502 SRX = SRX8689062 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6418515 spots for SRR12174197/SRR12174197.sra Written 6418515 spots for SRR12174197/SRR12174197.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170899 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:36 6418515 reads; of these: 6418515 (100.00%) were unpaired; of these: 751309 (11.71%) aligned 0 times 4822248 (75.13%) aligned exactly 1 time 844958 (13.16%) aligned >1 times 88.29% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 243461 / 5667206 = 0.0430 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:01:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:01:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:01:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:01:26: 1000000 INFO @ Sat, 11 Dec 2021 07:01:33: 2000000 INFO @ Sat, 11 Dec 2021 07:01:40: 3000000 INFO @ Sat, 11 Dec 2021 07:01:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:01:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:01:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:01:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:01:53: 5000000 INFO @ Sat, 11 Dec 2021 07:01:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:01:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:01:56: #1 total tags in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:01:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:01:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:01:56: #1 tags after filtering in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:01:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:01:56: #1 finished! INFO @ Sat, 11 Dec 2021 07:01:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:01:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:01:56: #2 number of paired peaks: 16 WARNING @ Sat, 11 Dec 2021 07:01:56: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:01:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:01:57: 1000000 INFO @ Sat, 11 Dec 2021 07:02:03: 2000000 INFO @ Sat, 11 Dec 2021 07:02:10: 3000000 INFO @ Sat, 11 Dec 2021 07:02:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:02:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:02:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:02:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:02:23: 5000000 INFO @ Sat, 11 Dec 2021 07:02:26: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:02:26: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:02:26: #1 total tags in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:02:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:02:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:02:26: #1 tags after filtering in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:02:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:02:26: #1 finished! INFO @ Sat, 11 Dec 2021 07:02:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:02:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:02:26: #2 number of paired peaks: 16 WARNING @ Sat, 11 Dec 2021 07:02:26: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:02:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:02:27: 1000000 INFO @ Sat, 11 Dec 2021 07:02:33: 2000000 INFO @ Sat, 11 Dec 2021 07:02:40: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:02:46: 4000000 INFO @ Sat, 11 Dec 2021 07:02:53: 5000000 INFO @ Sat, 11 Dec 2021 07:02:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:02:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:02:55: #1 total tags in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:02:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:02:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:02:55: #1 tags after filtering in treatment: 5423745 INFO @ Sat, 11 Dec 2021 07:02:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:02:55: #1 finished! INFO @ Sat, 11 Dec 2021 07:02:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:02:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:02:56: #2 number of paired peaks: 16 WARNING @ Sat, 11 Dec 2021 07:02:56: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:02:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689062/SRX8689062.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。