Job ID = 14170479 SRX = SRX8689040 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16898507 spots for SRR12174239/SRR12174239.sra Written 16898507 spots for SRR12174239/SRR12174239.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170908 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 16898507 reads; of these: 16898507 (100.00%) were unpaired; of these: 412412 (2.44%) aligned 0 times 12137648 (71.83%) aligned exactly 1 time 4348447 (25.73%) aligned >1 times 97.56% overall alignment rate Time searching: 00:06:55 Overall time: 00:06:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1015957 / 16486095 = 0.0616 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:07:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:07:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:07:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:07:45: 1000000 INFO @ Sat, 11 Dec 2021 07:07:56: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:08:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:08:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:08:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:08:07: 3000000 INFO @ Sat, 11 Dec 2021 07:08:12: 1000000 INFO @ Sat, 11 Dec 2021 07:08:18: 4000000 INFO @ Sat, 11 Dec 2021 07:08:21: 2000000 INFO @ Sat, 11 Dec 2021 07:08:30: 5000000 INFO @ Sat, 11 Dec 2021 07:08:30: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:08:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:08:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:08:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:08:40: 4000000 INFO @ Sat, 11 Dec 2021 07:08:42: 6000000 INFO @ Sat, 11 Dec 2021 07:08:43: 1000000 INFO @ Sat, 11 Dec 2021 07:08:49: 5000000 INFO @ Sat, 11 Dec 2021 07:08:53: 2000000 INFO @ Sat, 11 Dec 2021 07:08:53: 7000000 INFO @ Sat, 11 Dec 2021 07:08:59: 6000000 INFO @ Sat, 11 Dec 2021 07:09:02: 3000000 INFO @ Sat, 11 Dec 2021 07:09:05: 8000000 INFO @ Sat, 11 Dec 2021 07:09:08: 7000000 INFO @ Sat, 11 Dec 2021 07:09:12: 4000000 INFO @ Sat, 11 Dec 2021 07:09:15: 9000000 INFO @ Sat, 11 Dec 2021 07:09:18: 8000000 INFO @ Sat, 11 Dec 2021 07:09:22: 5000000 INFO @ Sat, 11 Dec 2021 07:09:27: 10000000 INFO @ Sat, 11 Dec 2021 07:09:27: 9000000 INFO @ Sat, 11 Dec 2021 07:09:32: 6000000 INFO @ Sat, 11 Dec 2021 07:09:36: 10000000 INFO @ Sat, 11 Dec 2021 07:09:39: 11000000 INFO @ Sat, 11 Dec 2021 07:09:42: 7000000 INFO @ Sat, 11 Dec 2021 07:09:46: 11000000 INFO @ Sat, 11 Dec 2021 07:09:51: 12000000 INFO @ Sat, 11 Dec 2021 07:09:52: 8000000 INFO @ Sat, 11 Dec 2021 07:09:57: 12000000 INFO @ Sat, 11 Dec 2021 07:10:02: 9000000 INFO @ Sat, 11 Dec 2021 07:10:02: 13000000 INFO @ Sat, 11 Dec 2021 07:10:07: 13000000 INFO @ Sat, 11 Dec 2021 07:10:11: 10000000 INFO @ Sat, 11 Dec 2021 07:10:13: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:10:16: 14000000 INFO @ Sat, 11 Dec 2021 07:10:21: 11000000 INFO @ Sat, 11 Dec 2021 07:10:24: 15000000 INFO @ Sat, 11 Dec 2021 07:10:26: 15000000 INFO @ Sat, 11 Dec 2021 07:10:29: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:10:29: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:10:29: #1 total tags in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:10:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:10:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:10:29: #1 tags after filtering in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:10:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:10:29: #1 finished! INFO @ Sat, 11 Dec 2021 07:10:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:10:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:10:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:10:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:10:30: #1 total tags in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:10:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:10:30: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 07:10:30: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:10:30: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 07:10:30: #1 tags after filtering in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:10:30: #1 finished! INFO @ Sat, 11 Dec 2021 07:10:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:10:30: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:10:31: 12000000 INFO @ Sat, 11 Dec 2021 07:10:32: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 07:10:32: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:10:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:10:40: 13000000 INFO @ Sat, 11 Dec 2021 07:10:49: 14000000 INFO @ Sat, 11 Dec 2021 07:10:57: 15000000 INFO @ Sat, 11 Dec 2021 07:11:01: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:11:01: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:11:01: #1 total tags in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:11:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:11:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:11:01: #1 tags after filtering in treatment: 15470138 INFO @ Sat, 11 Dec 2021 07:11:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:11:01: #1 finished! INFO @ Sat, 11 Dec 2021 07:11:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:11:01: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:11:03: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 07:11:03: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:11:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689040/SRX8689040.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling