Job ID = 14168248 SRX = SRX8688997 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5758584 spots for SRR12174188/SRR12174188.sra Written 5758584 spots for SRR12174188/SRR12174188.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169099 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:26 5758584 reads; of these: 5758584 (100.00%) were unpaired; of these: 474224 (8.24%) aligned 0 times 4623099 (80.28%) aligned exactly 1 time 661261 (11.48%) aligned >1 times 91.76% overall alignment rate Time searching: 00:01:26 Overall time: 00:01:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 157160 / 5284360 = 0.0297 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:37:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:37:38: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:37:38: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:37:44: 1000000 INFO @ Fri, 10 Dec 2021 16:37:50: 2000000 INFO @ Fri, 10 Dec 2021 16:37:56: 3000000 INFO @ Fri, 10 Dec 2021 16:38:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:38:07: 5000000 INFO @ Fri, 10 Dec 2021 16:38:08: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:38:08: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:38:08: #1 total tags in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:38:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:38:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:38:08: #1 tags after filtering in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:38:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:38:08: #1 finished! INFO @ Fri, 10 Dec 2021 16:38:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:38:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:38:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:38:08: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:38:08: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:38:09: #2 number of paired peaks: 32 WARNING @ Fri, 10 Dec 2021 16:38:09: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:38:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:38:15: 1000000 INFO @ Fri, 10 Dec 2021 16:38:22: 2000000 INFO @ Fri, 10 Dec 2021 16:38:29: 3000000 INFO @ Fri, 10 Dec 2021 16:38:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:38:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:38:38: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:38:38: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:38:42: 5000000 INFO @ Fri, 10 Dec 2021 16:38:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:38:43: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:38:43: #1 total tags in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:38:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:38:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:38:43: #1 tags after filtering in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:38:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:38:43: #1 finished! INFO @ Fri, 10 Dec 2021 16:38:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:38:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:38:43: #2 number of paired peaks: 32 WARNING @ Fri, 10 Dec 2021 16:38:43: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:38:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:38:46: 1000000 INFO @ Fri, 10 Dec 2021 16:38:53: 2000000 INFO @ Fri, 10 Dec 2021 16:39:00: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:39:07: 4000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:39:14: 5000000 INFO @ Fri, 10 Dec 2021 16:39:14: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:39:14: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:39:14: #1 total tags in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:39:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:39:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:39:15: #1 tags after filtering in treatment: 5127200 INFO @ Fri, 10 Dec 2021 16:39:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:39:15: #1 finished! INFO @ Fri, 10 Dec 2021 16:39:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:39:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:39:15: #2 number of paired peaks: 32 WARNING @ Fri, 10 Dec 2021 16:39:15: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:39:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688997/SRX8688997.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling