Job ID = 14168242 SRX = SRX8688993 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20535713 spots for SRR12174184/SRR12174184.sra Written 20535713 spots for SRR12174184/SRR12174184.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169110 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:50 20535713 reads; of these: 20535713 (100.00%) were unpaired; of these: 1099771 (5.36%) aligned 0 times 15631912 (76.12%) aligned exactly 1 time 3804030 (18.52%) aligned >1 times 94.64% overall alignment rate Time searching: 00:07:51 Overall time: 00:07:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1979666 / 19435942 = 0.1019 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:44:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:44:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:44:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:44:57: 1000000 INFO @ Fri, 10 Dec 2021 16:45:05: 2000000 INFO @ Fri, 10 Dec 2021 16:45:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:45:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:45:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:45:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:45:21: 4000000 INFO @ Fri, 10 Dec 2021 16:45:28: 5000000 INFO @ Fri, 10 Dec 2021 16:45:29: 1000000 INFO @ Fri, 10 Dec 2021 16:45:36: 6000000 INFO @ Fri, 10 Dec 2021 16:45:39: 2000000 INFO @ Fri, 10 Dec 2021 16:45:44: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:45:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:45:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:45:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:45:49: 3000000 INFO @ Fri, 10 Dec 2021 16:45:52: 8000000 INFO @ Fri, 10 Dec 2021 16:45:58: 1000000 INFO @ Fri, 10 Dec 2021 16:45:59: 4000000 INFO @ Fri, 10 Dec 2021 16:46:00: 9000000 INFO @ Fri, 10 Dec 2021 16:46:06: 2000000 INFO @ Fri, 10 Dec 2021 16:46:08: 10000000 INFO @ Fri, 10 Dec 2021 16:46:08: 5000000 INFO @ Fri, 10 Dec 2021 16:46:14: 3000000 INFO @ Fri, 10 Dec 2021 16:46:16: 11000000 INFO @ Fri, 10 Dec 2021 16:46:18: 6000000 INFO @ Fri, 10 Dec 2021 16:46:22: 4000000 INFO @ Fri, 10 Dec 2021 16:46:24: 12000000 INFO @ Fri, 10 Dec 2021 16:46:28: 7000000 INFO @ Fri, 10 Dec 2021 16:46:31: 5000000 INFO @ Fri, 10 Dec 2021 16:46:33: 13000000 INFO @ Fri, 10 Dec 2021 16:46:38: 8000000 INFO @ Fri, 10 Dec 2021 16:46:40: 6000000 INFO @ Fri, 10 Dec 2021 16:46:42: 14000000 INFO @ Fri, 10 Dec 2021 16:46:48: 9000000 INFO @ Fri, 10 Dec 2021 16:46:48: 7000000 INFO @ Fri, 10 Dec 2021 16:46:50: 15000000 INFO @ Fri, 10 Dec 2021 16:46:56: 8000000 INFO @ Fri, 10 Dec 2021 16:46:58: 10000000 INFO @ Fri, 10 Dec 2021 16:46:58: 16000000 INFO @ Fri, 10 Dec 2021 16:47:04: 9000000 INFO @ Fri, 10 Dec 2021 16:47:06: 17000000 INFO @ Fri, 10 Dec 2021 16:47:07: 11000000 INFO @ Fri, 10 Dec 2021 16:47:10: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:47:10: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:47:10: #1 total tags in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:47:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:47:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:47:11: #1 tags after filtering in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:47:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:47:11: #1 finished! INFO @ Fri, 10 Dec 2021 16:47:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:47:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:47:12: #2 number of paired peaks: 91 WARNING @ Fri, 10 Dec 2021 16:47:12: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:47:12: Process for pairing-model is terminated! INFO @ Fri, 10 Dec 2021 16:47:12: 10000000 cut: /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:47:17: 12000000 INFO @ Fri, 10 Dec 2021 16:47:20: 11000000 INFO @ Fri, 10 Dec 2021 16:47:26: 13000000 INFO @ Fri, 10 Dec 2021 16:47:28: 12000000 INFO @ Fri, 10 Dec 2021 16:47:36: 13000000 INFO @ Fri, 10 Dec 2021 16:47:36: 14000000 INFO @ Fri, 10 Dec 2021 16:47:43: 14000000 INFO @ Fri, 10 Dec 2021 16:47:46: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:47:51: 15000000 INFO @ Fri, 10 Dec 2021 16:47:55: 16000000 INFO @ Fri, 10 Dec 2021 16:47:59: 16000000 INFO @ Fri, 10 Dec 2021 16:48:05: 17000000 INFO @ Fri, 10 Dec 2021 16:48:07: 17000000 INFO @ Fri, 10 Dec 2021 16:48:09: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:48:09: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:48:09: #1 total tags in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:48:09: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:48:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:48:10: #1 tags after filtering in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:48:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:48:10: #1 finished! INFO @ Fri, 10 Dec 2021 16:48:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:48:11: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:48:11: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:48:11: #1 total tags in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:48:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:48:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:48:11: #2 number of paired peaks: 91 WARNING @ Fri, 10 Dec 2021 16:48:11: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:48:11: Process for pairing-model is terminated! INFO @ Fri, 10 Dec 2021 16:48:11: #1 tags after filtering in treatment: 17456276 INFO @ Fri, 10 Dec 2021 16:48:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:48:11: #1 finished! INFO @ Fri, 10 Dec 2021 16:48:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:48:11: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:48:13: #2 number of paired peaks: 91 WARNING @ Fri, 10 Dec 2021 16:48:13: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:48:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688993/SRX8688993.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。