Job ID = 14168241 SRX = SRX8688992 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20004097 spots for SRR12174183/SRR12174183.sra Written 20004097 spots for SRR12174183/SRR12174183.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169097 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:57 20004097 reads; of these: 20004097 (100.00%) were unpaired; of these: 965126 (4.82%) aligned 0 times 15045509 (75.21%) aligned exactly 1 time 3993462 (19.96%) aligned >1 times 95.18% overall alignment rate Time searching: 00:05:58 Overall time: 00:05:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1601966 / 19038971 = 0.0841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:39:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:39:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:39:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:39:40: 1000000 INFO @ Fri, 10 Dec 2021 16:39:45: 2000000 INFO @ Fri, 10 Dec 2021 16:39:51: 3000000 INFO @ Fri, 10 Dec 2021 16:39:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:40:02: 5000000 INFO @ Fri, 10 Dec 2021 16:40:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:40:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:40:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:40:09: 6000000 INFO @ Fri, 10 Dec 2021 16:40:12: 1000000 INFO @ Fri, 10 Dec 2021 16:40:17: 7000000 INFO @ Fri, 10 Dec 2021 16:40:20: 2000000 INFO @ Fri, 10 Dec 2021 16:40:24: 8000000 INFO @ Fri, 10 Dec 2021 16:40:29: 3000000 INFO @ Fri, 10 Dec 2021 16:40:31: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:40:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:40:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:40:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:40:37: 4000000 INFO @ Fri, 10 Dec 2021 16:40:38: 10000000 INFO @ Fri, 10 Dec 2021 16:40:42: 1000000 INFO @ Fri, 10 Dec 2021 16:40:45: 5000000 INFO @ Fri, 10 Dec 2021 16:40:46: 11000000 INFO @ Fri, 10 Dec 2021 16:40:50: 2000000 INFO @ Fri, 10 Dec 2021 16:40:53: 12000000 INFO @ Fri, 10 Dec 2021 16:40:53: 6000000 INFO @ Fri, 10 Dec 2021 16:40:58: 3000000 INFO @ Fri, 10 Dec 2021 16:41:00: 13000000 INFO @ Fri, 10 Dec 2021 16:41:01: 7000000 INFO @ Fri, 10 Dec 2021 16:41:06: 4000000 INFO @ Fri, 10 Dec 2021 16:41:08: 14000000 INFO @ Fri, 10 Dec 2021 16:41:10: 8000000 INFO @ Fri, 10 Dec 2021 16:41:14: 5000000 INFO @ Fri, 10 Dec 2021 16:41:15: 15000000 INFO @ Fri, 10 Dec 2021 16:41:18: 9000000 INFO @ Fri, 10 Dec 2021 16:41:22: 6000000 INFO @ Fri, 10 Dec 2021 16:41:22: 16000000 INFO @ Fri, 10 Dec 2021 16:41:26: 10000000 INFO @ Fri, 10 Dec 2021 16:41:30: 17000000 INFO @ Fri, 10 Dec 2021 16:41:30: 7000000 INFO @ Fri, 10 Dec 2021 16:41:33: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:41:33: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:41:33: #1 total tags in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:41:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:41:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:41:33: #1 tags after filtering in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:41:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:41:33: #1 finished! INFO @ Fri, 10 Dec 2021 16:41:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:41:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:41:34: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 16:41:34: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:41:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Fri, 10 Dec 2021 16:41:34: 11000000 rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:41:38: 8000000 INFO @ Fri, 10 Dec 2021 16:41:42: 12000000 INFO @ Fri, 10 Dec 2021 16:41:46: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:41:51: 13000000 INFO @ Fri, 10 Dec 2021 16:41:54: 10000000 INFO @ Fri, 10 Dec 2021 16:41:59: 14000000 INFO @ Fri, 10 Dec 2021 16:42:01: 11000000 INFO @ Fri, 10 Dec 2021 16:42:07: 15000000 INFO @ Fri, 10 Dec 2021 16:42:09: 12000000 INFO @ Fri, 10 Dec 2021 16:42:15: 16000000 INFO @ Fri, 10 Dec 2021 16:42:17: 13000000 INFO @ Fri, 10 Dec 2021 16:42:23: 17000000 INFO @ Fri, 10 Dec 2021 16:42:25: 14000000 INFO @ Fri, 10 Dec 2021 16:42:27: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:42:27: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:42:27: #1 total tags in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:42:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:42:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:42:27: #1 tags after filtering in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:42:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:42:27: #1 finished! INFO @ Fri, 10 Dec 2021 16:42:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:42:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:42:28: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 16:42:28: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:42:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:42:32: 15000000 INFO @ Fri, 10 Dec 2021 16:42:39: 16000000 INFO @ Fri, 10 Dec 2021 16:42:47: 17000000 INFO @ Fri, 10 Dec 2021 16:42:50: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:42:50: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:42:50: #1 total tags in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:42:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:42:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:42:50: #1 tags after filtering in treatment: 17437005 INFO @ Fri, 10 Dec 2021 16:42:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:42:50: #1 finished! INFO @ Fri, 10 Dec 2021 16:42:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:42:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:42:51: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 16:42:51: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:42:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688992/SRX8688992.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling