Job ID = 14168161 SRX = SRX8688984 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8483818 spots for SRR12174321/SRR12174321.sra Written 8483818 spots for SRR12174321/SRR12174321.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168967 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 8483818 reads; of these: 8483818 (100.00%) were unpaired; of these: 269594 (3.18%) aligned 0 times 6077386 (71.64%) aligned exactly 1 time 2136838 (25.19%) aligned >1 times 96.82% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 311992 / 8214224 = 0.0380 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:53:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:53:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:53:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:53:22: 1000000 INFO @ Fri, 10 Dec 2021 15:53:27: 2000000 INFO @ Fri, 10 Dec 2021 15:53:31: 3000000 INFO @ Fri, 10 Dec 2021 15:53:36: 4000000 INFO @ Fri, 10 Dec 2021 15:53:40: 5000000 INFO @ Fri, 10 Dec 2021 15:53:45: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:53:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:53:48: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:53:48: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:53:49: 7000000 INFO @ Fri, 10 Dec 2021 15:53:53: 1000000 INFO @ Fri, 10 Dec 2021 15:53:54: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:53:54: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:53:54: #1 total tags in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:53:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:53:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:53:54: #1 tags after filtering in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:53:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:53:54: #1 finished! INFO @ Fri, 10 Dec 2021 15:53:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:53:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:53:54: #2 number of paired peaks: 92 WARNING @ Fri, 10 Dec 2021 15:53:54: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:53:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:53:58: 2000000 INFO @ Fri, 10 Dec 2021 15:54:03: 3000000 INFO @ Fri, 10 Dec 2021 15:54:08: 4000000 INFO @ Fri, 10 Dec 2021 15:54:13: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:54:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:54:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:54:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:54:18: 6000000 INFO @ Fri, 10 Dec 2021 15:54:23: 1000000 INFO @ Fri, 10 Dec 2021 15:54:24: 7000000 INFO @ Fri, 10 Dec 2021 15:54:28: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:54:28: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:54:28: #1 total tags in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:54:28: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:54:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:54:28: 2000000 INFO @ Fri, 10 Dec 2021 15:54:28: #1 tags after filtering in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:54:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:54:28: #1 finished! INFO @ Fri, 10 Dec 2021 15:54:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:54:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:54:29: #2 number of paired peaks: 92 WARNING @ Fri, 10 Dec 2021 15:54:29: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:54:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:54:34: 3000000 INFO @ Fri, 10 Dec 2021 15:54:39: 4000000 INFO @ Fri, 10 Dec 2021 15:54:44: 5000000 INFO @ Fri, 10 Dec 2021 15:54:49: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:54:54: 7000000 INFO @ Fri, 10 Dec 2021 15:54:59: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:54:59: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:54:59: #1 total tags in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:54:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:54:59: #1 tags after filtering in treatment: 7902232 INFO @ Fri, 10 Dec 2021 15:54:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:54:59: #1 finished! INFO @ Fri, 10 Dec 2021 15:54:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:54:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:55:00: #2 number of paired peaks: 92 WARNING @ Fri, 10 Dec 2021 15:55:00: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:55:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688984/SRX8688984.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。