Job ID = 14168084 SRX = SRX8688978 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10671 spots for SRR12174347/SRR12174347.sra Written 10671 spots for SRR12174347/SRR12174347.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168820 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 10671 reads; of these: 10671 (100.00%) were unpaired; of these: 5702 (53.43%) aligned 0 times 3332 (31.22%) aligned exactly 1 time 1637 (15.34%) aligned >1 times 46.57% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 16 / 4969 = 0.0032 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:11:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:11:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:11:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:11:36: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:11:36: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:11:36: #1 total tags in treatment: 4953 INFO @ Fri, 10 Dec 2021 15:11:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:11:36: #1 tags after filtering in treatment: 4950 INFO @ Fri, 10 Dec 2021 15:11:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:11:36: #1 finished! INFO @ Fri, 10 Dec 2021 15:11:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:11:36: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 15:11:36: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:11:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:12:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:12:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:12:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:12:05: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:12:05: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:12:05: #1 total tags in treatment: 4953 INFO @ Fri, 10 Dec 2021 15:12:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:12:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:12:05: #1 tags after filtering in treatment: 4950 INFO @ Fri, 10 Dec 2021 15:12:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:12:05: #1 finished! INFO @ Fri, 10 Dec 2021 15:12:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:12:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:12:05: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 15:12:05: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:12:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 15:12:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:12:35: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:12:35: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:12:35: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:12:35: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:12:35: #1 total tags in treatment: 4953 INFO @ Fri, 10 Dec 2021 15:12:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:12:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:12:35: #1 tags after filtering in treatment: 4950 INFO @ Fri, 10 Dec 2021 15:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:12:35: #1 finished! INFO @ Fri, 10 Dec 2021 15:12:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:12:35: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 15:12:35: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:12:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688978/SRX8688978.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling