Job ID = 14168000 SRX = SRX8688966 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7713106 spots for SRR12174262/SRR12174262.sra Written 7713106 spots for SRR12174262/SRR12174262.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168546 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:38 7713106 reads; of these: 7713106 (100.00%) were unpaired; of these: 196486 (2.55%) aligned 0 times 5396294 (69.96%) aligned exactly 1 time 2120326 (27.49%) aligned >1 times 97.45% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 396305 / 7516620 = 0.0527 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:21:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:21:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:21:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:21:09: 1000000 INFO @ Fri, 10 Dec 2021 14:21:13: 2000000 INFO @ Fri, 10 Dec 2021 14:21:18: 3000000 INFO @ Fri, 10 Dec 2021 14:21:22: 4000000 INFO @ Fri, 10 Dec 2021 14:21:27: 5000000 INFO @ Fri, 10 Dec 2021 14:21:32: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:21:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:21:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:21:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:21:37: 7000000 INFO @ Fri, 10 Dec 2021 14:21:37: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:21:37: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:21:37: #1 total tags in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:21:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:21:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:21:37: #1 tags after filtering in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:21:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:21:37: #1 finished! INFO @ Fri, 10 Dec 2021 14:21:37: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:21:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:21:38: #2 number of paired peaks: 289 WARNING @ Fri, 10 Dec 2021 14:21:38: Fewer paired peaks (289) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 289 pairs to build model! INFO @ Fri, 10 Dec 2021 14:21:38: start model_add_line... INFO @ Fri, 10 Dec 2021 14:21:38: start X-correlation... INFO @ Fri, 10 Dec 2021 14:21:38: end of X-cor INFO @ Fri, 10 Dec 2021 14:21:38: #2 finished! INFO @ Fri, 10 Dec 2021 14:21:38: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 14:21:38: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 14:21:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05_model.r WARNING @ Fri, 10 Dec 2021 14:21:38: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:21:38: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 14:21:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:21:38: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:21:38: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:21:39: 1000000 INFO @ Fri, 10 Dec 2021 14:21:43: 2000000 INFO @ Fri, 10 Dec 2021 14:21:48: 3000000 INFO @ Fri, 10 Dec 2021 14:21:52: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:21:53: 4000000 INFO @ Fri, 10 Dec 2021 14:21:58: 5000000 INFO @ Fri, 10 Dec 2021 14:21:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05_peaks.xls INFO @ Fri, 10 Dec 2021 14:21:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:21:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.05_summits.bed INFO @ Fri, 10 Dec 2021 14:21:59: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (1279 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:22:02: 6000000 INFO @ Fri, 10 Dec 2021 14:22:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:22:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:22:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:22:07: 7000000 INFO @ Fri, 10 Dec 2021 14:22:08: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:22:08: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:22:08: #1 total tags in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:22:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:22:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:22:08: #1 tags after filtering in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:22:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:22:08: #1 finished! INFO @ Fri, 10 Dec 2021 14:22:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:22:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:22:08: #2 number of paired peaks: 289 WARNING @ Fri, 10 Dec 2021 14:22:08: Fewer paired peaks (289) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 289 pairs to build model! INFO @ Fri, 10 Dec 2021 14:22:08: start model_add_line... INFO @ Fri, 10 Dec 2021 14:22:08: start X-correlation... INFO @ Fri, 10 Dec 2021 14:22:08: end of X-cor INFO @ Fri, 10 Dec 2021 14:22:08: #2 finished! INFO @ Fri, 10 Dec 2021 14:22:08: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 14:22:08: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 14:22:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10_model.r WARNING @ Fri, 10 Dec 2021 14:22:08: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:22:08: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 14:22:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:22:08: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:22:08: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:22:09: 1000000 INFO @ Fri, 10 Dec 2021 14:22:13: 2000000 INFO @ Fri, 10 Dec 2021 14:22:18: 3000000 INFO @ Fri, 10 Dec 2021 14:22:22: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:22:23: 4000000 INFO @ Fri, 10 Dec 2021 14:22:28: 5000000 INFO @ Fri, 10 Dec 2021 14:22:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10_peaks.xls INFO @ Fri, 10 Dec 2021 14:22:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:22:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.10_summits.bed INFO @ Fri, 10 Dec 2021 14:22:30: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (1008 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:22:32: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 14:22:37: 7000000 INFO @ Fri, 10 Dec 2021 14:22:37: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:22:37: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:22:37: #1 total tags in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:22:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:22:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:22:38: #1 tags after filtering in treatment: 7120315 INFO @ Fri, 10 Dec 2021 14:22:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:22:38: #1 finished! INFO @ Fri, 10 Dec 2021 14:22:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:22:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:22:38: #2 number of paired peaks: 289 WARNING @ Fri, 10 Dec 2021 14:22:38: Fewer paired peaks (289) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 289 pairs to build model! INFO @ Fri, 10 Dec 2021 14:22:38: start model_add_line... INFO @ Fri, 10 Dec 2021 14:22:38: start X-correlation... INFO @ Fri, 10 Dec 2021 14:22:38: end of X-cor INFO @ Fri, 10 Dec 2021 14:22:38: #2 finished! INFO @ Fri, 10 Dec 2021 14:22:38: #2 predicted fragment length is 49 bps INFO @ Fri, 10 Dec 2021 14:22:38: #2 alternative fragment length(s) may be 49 bps INFO @ Fri, 10 Dec 2021 14:22:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20_model.r WARNING @ Fri, 10 Dec 2021 14:22:38: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 14:22:38: #2 You may need to consider one of the other alternative d(s): 49 WARNING @ Fri, 10 Dec 2021 14:22:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 14:22:38: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:22:38: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:22:52: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 14:22:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20_peaks.xls INFO @ Fri, 10 Dec 2021 14:22:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:22:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688966/SRX8688966.20_summits.bed INFO @ Fri, 10 Dec 2021 14:22:59: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (754 records, 4 fields): 1 millis CompletedMACS2peakCalling