Job ID = 14167495 SRX = SRX8688955 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6313708 spots for SRR12174226/SRR12174226.sra Written 6313708 spots for SRR12174226/SRR12174226.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168076 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:07 6313708 reads; of these: 6313708 (100.00%) were unpaired; of these: 592662 (9.39%) aligned 0 times 4151712 (65.76%) aligned exactly 1 time 1569334 (24.86%) aligned >1 times 90.61% overall alignment rate Time searching: 00:02:07 Overall time: 00:02:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 312662 / 5721046 = 0.0547 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:31:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:31:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:31:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:31:22: 1000000 INFO @ Fri, 10 Dec 2021 12:31:29: 2000000 INFO @ Fri, 10 Dec 2021 12:31:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:31:43: 4000000 INFO @ Fri, 10 Dec 2021 12:31:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:31:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:31:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:31:51: 5000000 INFO @ Fri, 10 Dec 2021 12:31:51: 1000000 INFO @ Fri, 10 Dec 2021 12:31:54: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:31:54: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:31:54: #1 total tags in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:31:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:31:54: #1 tags after filtering in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:31:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:31:54: #1 finished! INFO @ Fri, 10 Dec 2021 12:31:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:31:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:31:55: #2 number of paired peaks: 235 WARNING @ Fri, 10 Dec 2021 12:31:55: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Dec 2021 12:31:55: start model_add_line... INFO @ Fri, 10 Dec 2021 12:31:55: start X-correlation... INFO @ Fri, 10 Dec 2021 12:31:55: end of X-cor INFO @ Fri, 10 Dec 2021 12:31:55: #2 finished! INFO @ Fri, 10 Dec 2021 12:31:55: #2 predicted fragment length is 52 bps INFO @ Fri, 10 Dec 2021 12:31:55: #2 alternative fragment length(s) may be 52 bps INFO @ Fri, 10 Dec 2021 12:31:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05_model.r WARNING @ Fri, 10 Dec 2021 12:31:55: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:31:55: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Fri, 10 Dec 2021 12:31:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:31:55: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:31:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:31:58: 2000000 INFO @ Fri, 10 Dec 2021 12:32:04: 3000000 INFO @ Fri, 10 Dec 2021 12:32:07: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:32:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:32:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05_peaks.xls INFO @ Fri, 10 Dec 2021 12:32:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:32:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.05_summits.bed INFO @ Fri, 10 Dec 2021 12:32:13: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (1265 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:32:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:32:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:32:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:32:16: 5000000 INFO @ Fri, 10 Dec 2021 12:32:19: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:32:19: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:32:19: #1 total tags in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:32:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:32:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:32:19: #1 tags after filtering in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:32:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:32:19: #1 finished! INFO @ Fri, 10 Dec 2021 12:32:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:32:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:32:20: #2 number of paired peaks: 235 WARNING @ Fri, 10 Dec 2021 12:32:20: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Dec 2021 12:32:20: start model_add_line... INFO @ Fri, 10 Dec 2021 12:32:20: start X-correlation... INFO @ Fri, 10 Dec 2021 12:32:20: end of X-cor INFO @ Fri, 10 Dec 2021 12:32:20: #2 finished! INFO @ Fri, 10 Dec 2021 12:32:20: #2 predicted fragment length is 52 bps INFO @ Fri, 10 Dec 2021 12:32:20: #2 alternative fragment length(s) may be 52 bps INFO @ Fri, 10 Dec 2021 12:32:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10_model.r WARNING @ Fri, 10 Dec 2021 12:32:20: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:32:20: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Fri, 10 Dec 2021 12:32:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:32:20: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:32:20: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:32:21: 1000000 INFO @ Fri, 10 Dec 2021 12:32:27: 2000000 INFO @ Fri, 10 Dec 2021 12:32:32: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:32:33: 3000000 INFO @ Fri, 10 Dec 2021 12:32:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10_peaks.xls INFO @ Fri, 10 Dec 2021 12:32:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:32:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.10_summits.bed INFO @ Fri, 10 Dec 2021 12:32:38: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (1079 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:32:38: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:32:44: 5000000 INFO @ Fri, 10 Dec 2021 12:32:47: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:32:47: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:32:47: #1 total tags in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:32:47: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:32:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:32:47: #1 tags after filtering in treatment: 5408384 INFO @ Fri, 10 Dec 2021 12:32:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:32:47: #1 finished! INFO @ Fri, 10 Dec 2021 12:32:47: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:32:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:32:47: #2 number of paired peaks: 235 WARNING @ Fri, 10 Dec 2021 12:32:47: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Dec 2021 12:32:47: start model_add_line... INFO @ Fri, 10 Dec 2021 12:32:47: start X-correlation... INFO @ Fri, 10 Dec 2021 12:32:47: end of X-cor INFO @ Fri, 10 Dec 2021 12:32:47: #2 finished! INFO @ Fri, 10 Dec 2021 12:32:47: #2 predicted fragment length is 52 bps INFO @ Fri, 10 Dec 2021 12:32:47: #2 alternative fragment length(s) may be 52 bps INFO @ Fri, 10 Dec 2021 12:32:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20_model.r WARNING @ Fri, 10 Dec 2021 12:32:47: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:32:47: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Fri, 10 Dec 2021 12:32:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:32:47: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:32:47: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:32:59: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:33:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20_peaks.xls INFO @ Fri, 10 Dec 2021 12:33:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:33:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8688955/SRX8688955.20_summits.bed INFO @ Fri, 10 Dec 2021 12:33:06: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (743 records, 4 fields): 2 millis CompletedMACS2peakCalling