Job ID = 14167478 SRX = SRX8688952 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6776950 spots for SRR12174223/SRR12174223.sra Written 6776950 spots for SRR12174223/SRR12174223.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168067 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:55 6776950 reads; of these: 6776950 (100.00%) were unpaired; of these: 900855 (13.29%) aligned 0 times 4597175 (67.84%) aligned exactly 1 time 1278920 (18.87%) aligned >1 times 86.71% overall alignment rate Time searching: 00:01:55 Overall time: 00:01:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 232797 / 5876095 = 0.0396 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:13: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:13: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:19: 1000000 INFO @ Fri, 10 Dec 2021 12:28:25: 2000000 INFO @ Fri, 10 Dec 2021 12:28:30: 3000000 INFO @ Fri, 10 Dec 2021 12:28:35: 4000000 INFO @ Fri, 10 Dec 2021 12:28:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:43: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:43: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:44: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:44: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:44: #1 total tags in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:28:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:45: #1 tags after filtering in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:28:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:45: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:45: #2 number of paired peaks: 75 WARNING @ Fri, 10 Dec 2021 12:28:45: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:50: 1000000 INFO @ Fri, 10 Dec 2021 12:28:56: 2000000 INFO @ Fri, 10 Dec 2021 12:29:03: 3000000 INFO @ Fri, 10 Dec 2021 12:29:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:29:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:29:13: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:29:13: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:29:16: 5000000 INFO @ Fri, 10 Dec 2021 12:29:20: 1000000 INFO @ Fri, 10 Dec 2021 12:29:20: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:20: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:20: #1 total tags in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:29:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:20: #1 tags after filtering in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:29:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:20: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:20: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:21: #2 number of paired peaks: 75 WARNING @ Fri, 10 Dec 2021 12:29:21: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:29:25: 2000000 INFO @ Fri, 10 Dec 2021 12:29:31: 3000000 INFO @ Fri, 10 Dec 2021 12:29:36: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:29:42: 5000000 INFO @ Fri, 10 Dec 2021 12:29:46: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:46: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:46: #1 total tags in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:29:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:46: #1 tags after filtering in treatment: 5643298 INFO @ Fri, 10 Dec 2021 12:29:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:46: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:46: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:46: #2 number of paired peaks: 75 WARNING @ Fri, 10 Dec 2021 12:29:46: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688952/SRX8688952.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。