Job ID = 14167477 SRX = SRX8688951 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6593649 spots for SRR12174222/SRR12174222.sra Written 6593649 spots for SRR12174222/SRR12174222.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168065 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 6593649 reads; of these: 6593649 (100.00%) were unpaired; of these: 726990 (11.03%) aligned 0 times 4708957 (71.42%) aligned exactly 1 time 1157702 (17.56%) aligned >1 times 88.97% overall alignment rate Time searching: 00:01:40 Overall time: 00:01:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 241172 / 5866659 = 0.0411 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:00: 1000000 INFO @ Fri, 10 Dec 2021 12:28:05: 2000000 INFO @ Fri, 10 Dec 2021 12:28:10: 3000000 INFO @ Fri, 10 Dec 2021 12:28:15: 4000000 INFO @ Fri, 10 Dec 2021 12:28:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:23: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:23: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:23: #1 total tags in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:28:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:24: #1 tags after filtering in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:28:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:24: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:24: #2 number of paired peaks: 68 WARNING @ Fri, 10 Dec 2021 12:28:24: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:31: 1000000 INFO @ Fri, 10 Dec 2021 12:28:37: 2000000 INFO @ Fri, 10 Dec 2021 12:28:43: 3000000 INFO @ Fri, 10 Dec 2021 12:28:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:56: 5000000 INFO @ Fri, 10 Dec 2021 12:29:00: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:00: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:00: #1 total tags in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:29:00: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:00: #1 tags after filtering in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:29:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:00: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:00: 1000000 INFO @ Fri, 10 Dec 2021 12:29:00: #2 number of paired peaks: 68 WARNING @ Fri, 10 Dec 2021 12:29:00: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:29:06: 2000000 INFO @ Fri, 10 Dec 2021 12:29:11: 3000000 INFO @ Fri, 10 Dec 2021 12:29:17: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:29:22: 5000000 INFO @ Fri, 10 Dec 2021 12:29:25: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:25: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:25: #1 total tags in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:29:25: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:25: #1 tags after filtering in treatment: 5625487 INFO @ Fri, 10 Dec 2021 12:29:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:25: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:26: #2 number of paired peaks: 68 WARNING @ Fri, 10 Dec 2021 12:29:26: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688951/SRX8688951.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。