Job ID = 14167432 SRX = SRX8688943 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21669635 spots for SRR12174174/SRR12174174.sra Written 21669635 spots for SRR12174174/SRR12174174.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168051 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:16 21669635 reads; of these: 21669635 (100.00%) were unpaired; of these: 6858257 (31.65%) aligned 0 times 11458722 (52.88%) aligned exactly 1 time 3352656 (15.47%) aligned >1 times 68.35% overall alignment rate Time searching: 00:05:16 Overall time: 00:05:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 974577 / 14811378 = 0.0658 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:11: 1000000 INFO @ Fri, 10 Dec 2021 12:27:16: 2000000 INFO @ Fri, 10 Dec 2021 12:27:22: 3000000 INFO @ Fri, 10 Dec 2021 12:27:27: 4000000 INFO @ Fri, 10 Dec 2021 12:27:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:37: 6000000 INFO @ Fri, 10 Dec 2021 12:27:41: 1000000 INFO @ Fri, 10 Dec 2021 12:27:43: 7000000 INFO @ Fri, 10 Dec 2021 12:27:46: 2000000 INFO @ Fri, 10 Dec 2021 12:27:48: 8000000 INFO @ Fri, 10 Dec 2021 12:27:50: 3000000 INFO @ Fri, 10 Dec 2021 12:27:53: 9000000 INFO @ Fri, 10 Dec 2021 12:27:55: 4000000 INFO @ Fri, 10 Dec 2021 12:27:59: 10000000 INFO @ Fri, 10 Dec 2021 12:28:00: 5000000 BedGraph に変換中... INFO @ Fri, 10 Dec 2021 12:28:04: 11000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:04: 6000000 INFO @ Fri, 10 Dec 2021 12:28:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:09: 7000000 INFO @ Fri, 10 Dec 2021 12:28:09: 12000000 INFO @ Fri, 10 Dec 2021 12:28:11: 1000000 INFO @ Fri, 10 Dec 2021 12:28:14: 8000000 INFO @ Fri, 10 Dec 2021 12:28:14: 13000000 INFO @ Fri, 10 Dec 2021 12:28:17: 2000000 INFO @ Fri, 10 Dec 2021 12:28:19: 9000000 INFO @ Fri, 10 Dec 2021 12:28:19: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:19: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:19: #1 total tags in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:28:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:19: #1 tags after filtering in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:28:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:19: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:20: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 12:28:20: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:22: 3000000 INFO @ Fri, 10 Dec 2021 12:28:23: 10000000 INFO @ Fri, 10 Dec 2021 12:28:27: 4000000 INFO @ Fri, 10 Dec 2021 12:28:28: 11000000 INFO @ Fri, 10 Dec 2021 12:28:32: 5000000 INFO @ Fri, 10 Dec 2021 12:28:33: 12000000 INFO @ Fri, 10 Dec 2021 12:28:37: 13000000 INFO @ Fri, 10 Dec 2021 12:28:38: 6000000 INFO @ Fri, 10 Dec 2021 12:28:41: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:41: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:41: #1 total tags in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:28:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:41: #1 tags after filtering in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:28:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:41: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:42: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 12:28:42: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:43: 7000000 INFO @ Fri, 10 Dec 2021 12:28:49: 8000000 INFO @ Fri, 10 Dec 2021 12:28:54: 9000000 INFO @ Fri, 10 Dec 2021 12:28:59: 10000000 INFO @ Fri, 10 Dec 2021 12:29:05: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:29:10: 12000000 INFO @ Fri, 10 Dec 2021 12:29:15: 13000000 INFO @ Fri, 10 Dec 2021 12:29:20: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:20: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:20: #1 total tags in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:29:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:20: #1 tags after filtering in treatment: 13836801 INFO @ Fri, 10 Dec 2021 12:29:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:20: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:20: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:21: #2 number of paired peaks: 70 WARNING @ Fri, 10 Dec 2021 12:29:21: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688943/SRX8688943.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。