Job ID = 14167427 SRX = SRX8688941 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21177474 spots for SRR12174294/SRR12174294.sra Written 21177474 spots for SRR12174294/SRR12174294.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168072 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:15 21177474 reads; of these: 21177474 (100.00%) were unpaired; of these: 539525 (2.55%) aligned 0 times 14802852 (69.90%) aligned exactly 1 time 5835097 (27.55%) aligned >1 times 97.45% overall alignment rate Time searching: 00:09:15 Overall time: 00:09:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1919751 / 20637949 = 0.0930 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:33:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:33:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:33:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:34:04: 1000000 INFO @ Fri, 10 Dec 2021 12:34:12: 2000000 INFO @ Fri, 10 Dec 2021 12:34:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:34:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:34:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:34:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:34:29: 4000000 INFO @ Fri, 10 Dec 2021 12:34:37: 1000000 INFO @ Fri, 10 Dec 2021 12:34:38: 5000000 INFO @ Fri, 10 Dec 2021 12:34:47: 6000000 INFO @ Fri, 10 Dec 2021 12:34:50: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:34:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:34:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:34:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:34:56: 7000000 INFO @ Fri, 10 Dec 2021 12:35:04: 3000000 INFO @ Fri, 10 Dec 2021 12:35:05: 8000000 INFO @ Fri, 10 Dec 2021 12:35:07: 1000000 INFO @ Fri, 10 Dec 2021 12:35:14: 9000000 INFO @ Fri, 10 Dec 2021 12:35:17: 4000000 INFO @ Fri, 10 Dec 2021 12:35:19: 2000000 INFO @ Fri, 10 Dec 2021 12:35:22: 10000000 INFO @ Fri, 10 Dec 2021 12:35:30: 5000000 INFO @ Fri, 10 Dec 2021 12:35:31: 11000000 INFO @ Fri, 10 Dec 2021 12:35:32: 3000000 INFO @ Fri, 10 Dec 2021 12:35:40: 12000000 INFO @ Fri, 10 Dec 2021 12:35:43: 6000000 INFO @ Fri, 10 Dec 2021 12:35:45: 4000000 INFO @ Fri, 10 Dec 2021 12:35:50: 13000000 INFO @ Fri, 10 Dec 2021 12:35:56: 7000000 INFO @ Fri, 10 Dec 2021 12:35:58: 5000000 INFO @ Fri, 10 Dec 2021 12:35:59: 14000000 INFO @ Fri, 10 Dec 2021 12:36:08: 15000000 INFO @ Fri, 10 Dec 2021 12:36:09: 8000000 INFO @ Fri, 10 Dec 2021 12:36:11: 6000000 INFO @ Fri, 10 Dec 2021 12:36:17: 16000000 INFO @ Fri, 10 Dec 2021 12:36:22: 9000000 INFO @ Fri, 10 Dec 2021 12:36:24: 7000000 INFO @ Fri, 10 Dec 2021 12:36:26: 17000000 INFO @ Fri, 10 Dec 2021 12:36:35: 10000000 INFO @ Fri, 10 Dec 2021 12:36:35: 18000000 INFO @ Fri, 10 Dec 2021 12:36:37: 8000000 INFO @ Fri, 10 Dec 2021 12:36:42: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:36:42: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:36:42: #1 total tags in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:36:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:36:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:36:42: #1 tags after filtering in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:36:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:36:42: #1 finished! INFO @ Fri, 10 Dec 2021 12:36:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:36:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:36:44: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 12:36:44: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:36:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:36:48: 11000000 INFO @ Fri, 10 Dec 2021 12:36:49: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:37:01: 12000000 INFO @ Fri, 10 Dec 2021 12:37:02: 10000000 INFO @ Fri, 10 Dec 2021 12:37:14: 11000000 INFO @ Fri, 10 Dec 2021 12:37:14: 13000000 INFO @ Fri, 10 Dec 2021 12:37:26: 12000000 INFO @ Fri, 10 Dec 2021 12:37:27: 14000000 INFO @ Fri, 10 Dec 2021 12:37:37: 13000000 INFO @ Fri, 10 Dec 2021 12:37:40: 15000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:37:49: 14000000 INFO @ Fri, 10 Dec 2021 12:37:54: 16000000 INFO @ Fri, 10 Dec 2021 12:38:01: 15000000 INFO @ Fri, 10 Dec 2021 12:38:07: 17000000 INFO @ Fri, 10 Dec 2021 12:38:13: 16000000 INFO @ Fri, 10 Dec 2021 12:38:20: 18000000 INFO @ Fri, 10 Dec 2021 12:38:25: 17000000 INFO @ Fri, 10 Dec 2021 12:38:28: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:38:28: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:38:28: #1 total tags in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:38:28: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:38:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:38:28: #1 tags after filtering in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:38:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:38:28: #1 finished! INFO @ Fri, 10 Dec 2021 12:38:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:38:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:38:29: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 12:38:29: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:38:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:38:37: 18000000 INFO @ Fri, 10 Dec 2021 12:38:45: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:38:45: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:38:45: #1 total tags in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:38:45: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:38:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:38:45: #1 tags after filtering in treatment: 18718198 INFO @ Fri, 10 Dec 2021 12:38:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:38:45: #1 finished! INFO @ Fri, 10 Dec 2021 12:38:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:38:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:38:47: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 12:38:47: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:38:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688941/SRX8688941.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling