Job ID = 14167363 SRX = SRX8688934 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23264978 spots for SRR12174286/SRR12174286.sra Written 23264978 spots for SRR12174286/SRR12174286.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168016 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 23264978 reads; of these: 23264978 (100.00%) were unpaired; of these: 701857 (3.02%) aligned 0 times 16182110 (69.56%) aligned exactly 1 time 6381011 (27.43%) aligned >1 times 96.98% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2303976 / 22563121 = 0.1021 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:18:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:18:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:18:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:18:39: 1000000 INFO @ Fri, 10 Dec 2021 12:18:43: 2000000 INFO @ Fri, 10 Dec 2021 12:18:48: 3000000 INFO @ Fri, 10 Dec 2021 12:18:53: 4000000 INFO @ Fri, 10 Dec 2021 12:18:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:19:03: 6000000 INFO @ Fri, 10 Dec 2021 12:19:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:19:03: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:19:03: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:19:08: 7000000 INFO @ Fri, 10 Dec 2021 12:19:08: 1000000 INFO @ Fri, 10 Dec 2021 12:19:13: 8000000 INFO @ Fri, 10 Dec 2021 12:19:13: 2000000 INFO @ Fri, 10 Dec 2021 12:19:17: 9000000 INFO @ Fri, 10 Dec 2021 12:19:18: 3000000 INFO @ Fri, 10 Dec 2021 12:19:22: 10000000 INFO @ Fri, 10 Dec 2021 12:19:23: 4000000 INFO @ Fri, 10 Dec 2021 12:19:27: 11000000 INFO @ Fri, 10 Dec 2021 12:19:28: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:19:32: 12000000 INFO @ Fri, 10 Dec 2021 12:19:33: 6000000 INFO @ Fri, 10 Dec 2021 12:19:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:19:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:19:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:19:37: 13000000 INFO @ Fri, 10 Dec 2021 12:19:38: 7000000 INFO @ Fri, 10 Dec 2021 12:19:39: 1000000 INFO @ Fri, 10 Dec 2021 12:19:42: 14000000 INFO @ Fri, 10 Dec 2021 12:19:43: 8000000 INFO @ Fri, 10 Dec 2021 12:19:44: 2000000 INFO @ Fri, 10 Dec 2021 12:19:47: 15000000 INFO @ Fri, 10 Dec 2021 12:19:48: 9000000 INFO @ Fri, 10 Dec 2021 12:19:49: 3000000 INFO @ Fri, 10 Dec 2021 12:19:52: 16000000 INFO @ Fri, 10 Dec 2021 12:19:53: 10000000 INFO @ Fri, 10 Dec 2021 12:19:54: 4000000 INFO @ Fri, 10 Dec 2021 12:19:57: 17000000 INFO @ Fri, 10 Dec 2021 12:19:58: 11000000 INFO @ Fri, 10 Dec 2021 12:19:59: 5000000 INFO @ Fri, 10 Dec 2021 12:20:02: 18000000 INFO @ Fri, 10 Dec 2021 12:20:03: 12000000 INFO @ Fri, 10 Dec 2021 12:20:04: 6000000 INFO @ Fri, 10 Dec 2021 12:20:07: 19000000 INFO @ Fri, 10 Dec 2021 12:20:08: 13000000 INFO @ Fri, 10 Dec 2021 12:20:09: 7000000 INFO @ Fri, 10 Dec 2021 12:20:12: 20000000 INFO @ Fri, 10 Dec 2021 12:20:13: 14000000 INFO @ Fri, 10 Dec 2021 12:20:13: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:20:13: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:20:13: #1 total tags in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:20:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:20:14: 8000000 INFO @ Fri, 10 Dec 2021 12:20:14: #1 tags after filtering in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:20:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:20:14: #1 finished! INFO @ Fri, 10 Dec 2021 12:20:14: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:20:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:20:15: #2 number of paired peaks: 90 WARNING @ Fri, 10 Dec 2021 12:20:15: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:20:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:20:18: 15000000 INFO @ Fri, 10 Dec 2021 12:20:19: 9000000 INFO @ Fri, 10 Dec 2021 12:20:23: 16000000 INFO @ Fri, 10 Dec 2021 12:20:24: 10000000 INFO @ Fri, 10 Dec 2021 12:20:28: 17000000 INFO @ Fri, 10 Dec 2021 12:20:29: 11000000 INFO @ Fri, 10 Dec 2021 12:20:33: 18000000 INFO @ Fri, 10 Dec 2021 12:20:34: 12000000 INFO @ Fri, 10 Dec 2021 12:20:38: 19000000 INFO @ Fri, 10 Dec 2021 12:20:39: 13000000 INFO @ Fri, 10 Dec 2021 12:20:43: 20000000 INFO @ Fri, 10 Dec 2021 12:20:44: 14000000 INFO @ Fri, 10 Dec 2021 12:20:44: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:20:44: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:20:44: #1 total tags in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:20:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:20:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:20:44: #1 tags after filtering in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:20:44: #1 finished! INFO @ Fri, 10 Dec 2021 12:20:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:20:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:20:46: #2 number of paired peaks: 90 WARNING @ Fri, 10 Dec 2021 12:20:46: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:20:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:20:49: 15000000 INFO @ Fri, 10 Dec 2021 12:20:54: 16000000 INFO @ Fri, 10 Dec 2021 12:20:59: 17000000 INFO @ Fri, 10 Dec 2021 12:21:03: 18000000 INFO @ Fri, 10 Dec 2021 12:21:08: 19000000 INFO @ Fri, 10 Dec 2021 12:21:13: 20000000 INFO @ Fri, 10 Dec 2021 12:21:15: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:21:15: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:21:15: #1 total tags in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:21:15: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:21:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:21:15: #1 tags after filtering in treatment: 20259145 INFO @ Fri, 10 Dec 2021 12:21:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:21:15: #1 finished! INFO @ Fri, 10 Dec 2021 12:21:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:21:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:21:17: #2 number of paired peaks: 90 WARNING @ Fri, 10 Dec 2021 12:21:17: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:21:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688934/SRX8688934.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。