Job ID = 14167265 SRX = SRX8688920 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13097216 spots for SRR12174378/SRR12174378.sra Written 13097216 spots for SRR12174378/SRR12174378.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167879 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:25 13097216 reads; of these: 13097216 (100.00%) were unpaired; of these: 385372 (2.94%) aligned 0 times 9922200 (75.76%) aligned exactly 1 time 2789644 (21.30%) aligned >1 times 97.06% overall alignment rate Time searching: 00:04:25 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1464926 / 12711844 = 0.1152 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:34:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:34:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:34:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:34:32: 1000000 INFO @ Fri, 10 Dec 2021 11:34:39: 2000000 INFO @ Fri, 10 Dec 2021 11:34:45: 3000000 INFO @ Fri, 10 Dec 2021 11:34:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:34:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:34:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:34:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:34:58: 5000000 INFO @ Fri, 10 Dec 2021 11:35:02: 1000000 INFO @ Fri, 10 Dec 2021 11:35:04: 6000000 INFO @ Fri, 10 Dec 2021 11:35:09: 2000000 INFO @ Fri, 10 Dec 2021 11:35:11: 7000000 INFO @ Fri, 10 Dec 2021 11:35:15: 3000000 INFO @ Fri, 10 Dec 2021 11:35:17: 8000000 INFO @ Fri, 10 Dec 2021 11:35:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:35:24: 9000000 INFO @ Fri, 10 Dec 2021 11:35:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:35:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:35:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:35:29: 5000000 INFO @ Fri, 10 Dec 2021 11:35:31: 10000000 INFO @ Fri, 10 Dec 2021 11:35:33: 1000000 INFO @ Fri, 10 Dec 2021 11:35:35: 6000000 INFO @ Fri, 10 Dec 2021 11:35:38: 11000000 INFO @ Fri, 10 Dec 2021 11:35:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:35:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:35:40: #1 total tags in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:35:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:35:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:35:40: #1 tags after filtering in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:35:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:35:40: #1 finished! INFO @ Fri, 10 Dec 2021 11:35:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:35:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:35:40: 2000000 INFO @ Fri, 10 Dec 2021 11:35:41: #2 number of paired peaks: 99 WARNING @ Fri, 10 Dec 2021 11:35:41: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:35:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:35:42: 7000000 INFO @ Fri, 10 Dec 2021 11:35:47: 3000000 INFO @ Fri, 10 Dec 2021 11:35:48: 8000000 INFO @ Fri, 10 Dec 2021 11:35:53: 4000000 INFO @ Fri, 10 Dec 2021 11:35:55: 9000000 INFO @ Fri, 10 Dec 2021 11:36:00: 5000000 INFO @ Fri, 10 Dec 2021 11:36:01: 10000000 INFO @ Fri, 10 Dec 2021 11:36:07: 6000000 INFO @ Fri, 10 Dec 2021 11:36:08: 11000000 INFO @ Fri, 10 Dec 2021 11:36:09: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:36:09: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:36:09: #1 total tags in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:36:09: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:36:10: #1 tags after filtering in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:36:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:36:10: #1 finished! INFO @ Fri, 10 Dec 2021 11:36:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:36:10: #2 number of paired peaks: 99 WARNING @ Fri, 10 Dec 2021 11:36:10: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:36:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:36:13: 7000000 INFO @ Fri, 10 Dec 2021 11:36:19: 8000000 INFO @ Fri, 10 Dec 2021 11:36:25: 9000000 INFO @ Fri, 10 Dec 2021 11:36:31: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:36:37: 11000000 INFO @ Fri, 10 Dec 2021 11:36:39: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:36:39: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:36:39: #1 total tags in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:36:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:36:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:36:39: #1 tags after filtering in treatment: 11246918 INFO @ Fri, 10 Dec 2021 11:36:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:36:39: #1 finished! INFO @ Fri, 10 Dec 2021 11:36:39: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:36:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:36:40: #2 number of paired peaks: 99 WARNING @ Fri, 10 Dec 2021 11:36:40: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:36:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688920/SRX8688920.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。