Job ID = 14168373 SRX = SRX8688896 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6245607 spots for SRR12174207/SRR12174207.sra Written 6245607 spots for SRR12174207/SRR12174207.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169541 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:49 6245607 reads; of these: 6245607 (100.00%) were unpaired; of these: 584220 (9.35%) aligned 0 times 4716197 (75.51%) aligned exactly 1 time 945190 (15.13%) aligned >1 times 90.65% overall alignment rate Time searching: 00:01:49 Overall time: 00:01:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 184958 / 5661387 = 0.0327 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:16:23: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:16:23: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:16:29: 1000000 INFO @ Fri, 10 Dec 2021 18:16:35: 2000000 INFO @ Fri, 10 Dec 2021 18:16:41: 3000000 INFO @ Fri, 10 Dec 2021 18:16:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:16:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:16:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:16:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:16:53: 5000000 INFO @ Fri, 10 Dec 2021 18:16:56: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:16:56: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:16:56: #1 total tags in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:16:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:16:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:16:56: #1 tags after filtering in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:16:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:16:56: #1 finished! INFO @ Fri, 10 Dec 2021 18:16:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:16:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:16:57: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 18:16:57: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:16:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:17:00: 1000000 INFO @ Fri, 10 Dec 2021 18:17:07: 2000000 INFO @ Fri, 10 Dec 2021 18:17:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:17:21: 4000000 INFO @ Fri, 10 Dec 2021 18:17:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:17:23: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:17:23: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:17:29: 5000000 INFO @ Fri, 10 Dec 2021 18:17:30: 1000000 INFO @ Fri, 10 Dec 2021 18:17:33: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:17:33: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:17:33: #1 total tags in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:17:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:17:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:17:33: #1 tags after filtering in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:17:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:17:33: #1 finished! INFO @ Fri, 10 Dec 2021 18:17:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:17:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:17:33: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 18:17:33: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:17:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:17:38: 2000000 INFO @ Fri, 10 Dec 2021 18:17:45: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 18:17:52: 4000000 INFO @ Fri, 10 Dec 2021 18:17:59: 5000000 INFO @ Fri, 10 Dec 2021 18:18:02: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:18:02: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:18:02: #1 total tags in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:18:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:18:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:18:02: #1 tags after filtering in treatment: 5476429 INFO @ Fri, 10 Dec 2021 18:18:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:18:02: #1 finished! INFO @ Fri, 10 Dec 2021 18:18:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:18:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:18:03: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 18:18:03: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:18:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688896/SRX8688896.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。