Job ID = 14168370 SRX = SRX8688893 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23752209 spots for SRR12174172/SRR12174172.sra Written 23752209 spots for SRR12174172/SRR12174172.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169559 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:58 23752209 reads; of these: 23752209 (100.00%) were unpaired; of these: 5851921 (24.64%) aligned 0 times 13858252 (58.35%) aligned exactly 1 time 4042036 (17.02%) aligned >1 times 75.36% overall alignment rate Time searching: 00:05:58 Overall time: 00:05:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1310409 / 17900288 = 0.0732 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:23:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:23:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:23:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:23:30: 1000000 INFO @ Fri, 10 Dec 2021 18:23:35: 2000000 INFO @ Fri, 10 Dec 2021 18:23:41: 3000000 INFO @ Fri, 10 Dec 2021 18:23:46: 4000000 INFO @ Fri, 10 Dec 2021 18:23:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:23:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:23:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:23:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:23:56: 6000000 INFO @ Fri, 10 Dec 2021 18:23:59: 1000000 INFO @ Fri, 10 Dec 2021 18:24:02: 7000000 INFO @ Fri, 10 Dec 2021 18:24:05: 2000000 INFO @ Fri, 10 Dec 2021 18:24:07: 8000000 INFO @ Fri, 10 Dec 2021 18:24:10: 3000000 INFO @ Fri, 10 Dec 2021 18:24:13: 9000000 INFO @ Fri, 10 Dec 2021 18:24:16: 4000000 INFO @ Fri, 10 Dec 2021 18:24:18: 10000000 INFO @ Fri, 10 Dec 2021 18:24:21: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:24:24: 11000000 INFO @ Fri, 10 Dec 2021 18:24:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:24:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:24:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:24:26: 6000000 INFO @ Fri, 10 Dec 2021 18:24:29: 12000000 INFO @ Fri, 10 Dec 2021 18:24:30: 1000000 INFO @ Fri, 10 Dec 2021 18:24:32: 7000000 INFO @ Fri, 10 Dec 2021 18:24:35: 13000000 INFO @ Fri, 10 Dec 2021 18:24:36: 2000000 INFO @ Fri, 10 Dec 2021 18:24:37: 8000000 INFO @ Fri, 10 Dec 2021 18:24:40: 14000000 INFO @ Fri, 10 Dec 2021 18:24:42: 9000000 INFO @ Fri, 10 Dec 2021 18:24:43: 3000000 INFO @ Fri, 10 Dec 2021 18:24:46: 15000000 INFO @ Fri, 10 Dec 2021 18:24:48: 10000000 INFO @ Fri, 10 Dec 2021 18:24:49: 4000000 INFO @ Fri, 10 Dec 2021 18:24:51: 16000000 INFO @ Fri, 10 Dec 2021 18:24:53: 11000000 INFO @ Fri, 10 Dec 2021 18:24:54: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:24:54: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:24:54: #1 total tags in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:24:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:24:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:24:55: #1 tags after filtering in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:24:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:24:55: #1 finished! INFO @ Fri, 10 Dec 2021 18:24:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:24:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:24:55: 5000000 INFO @ Fri, 10 Dec 2021 18:24:56: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 18:24:56: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:24:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:24:59: 12000000 INFO @ Fri, 10 Dec 2021 18:25:01: 6000000 INFO @ Fri, 10 Dec 2021 18:25:04: 13000000 INFO @ Fri, 10 Dec 2021 18:25:07: 7000000 INFO @ Fri, 10 Dec 2021 18:25:10: 14000000 INFO @ Fri, 10 Dec 2021 18:25:13: 8000000 INFO @ Fri, 10 Dec 2021 18:25:15: 15000000 INFO @ Fri, 10 Dec 2021 18:25:19: 9000000 INFO @ Fri, 10 Dec 2021 18:25:20: 16000000 INFO @ Fri, 10 Dec 2021 18:25:24: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:25:24: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:25:24: #1 total tags in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:25:24: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:25:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:25:24: #1 tags after filtering in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:25:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:25:24: #1 finished! INFO @ Fri, 10 Dec 2021 18:25:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:25:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:25:25: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 18:25:25: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:25:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 18:25:25: 10000000 INFO @ Fri, 10 Dec 2021 18:25:31: 11000000 INFO @ Fri, 10 Dec 2021 18:25:37: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 18:25:43: 13000000 INFO @ Fri, 10 Dec 2021 18:25:49: 14000000 INFO @ Fri, 10 Dec 2021 18:25:54: 15000000 INFO @ Fri, 10 Dec 2021 18:26:00: 16000000 INFO @ Fri, 10 Dec 2021 18:26:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 18:26:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 18:26:04: #1 total tags in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:26:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:26:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:26:04: #1 tags after filtering in treatment: 16589879 INFO @ Fri, 10 Dec 2021 18:26:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 18:26:04: #1 finished! INFO @ Fri, 10 Dec 2021 18:26:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:26:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:26:05: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 18:26:05: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 18:26:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688893/SRX8688893.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。