Job ID = 9159930 sra ファイルのダウンロード中... Completed: 286070K bytes transferred in 5 seconds (394406K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 15429368 spots for /home/okishinya/chipatlas/results/dm3/SRX859012/SRR1779568.sra Written 15429368 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:19 15429368 reads; of these: 15429368 (100.00%) were unpaired; of these: 71625 (0.46%) aligned 0 times 14741733 (95.54%) aligned exactly 1 time 616010 (3.99%) aligned >1 times 99.54% overall alignment rate Time searching: 00:03:19 Overall time: 00:03:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 596 / 15357743 = 0.0000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:15:35: # Command line: callpeak -t SRX859012.bam -f BAM -g dm -n SRX859012.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX859012.10 # format = BAM # ChIP-seq file = ['SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:15:35: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:15:35: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:15:35: # Command line: callpeak -t SRX859012.bam -f BAM -g dm -n SRX859012.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX859012.20 # format = BAM # ChIP-seq file = ['SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:15:35: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:15:35: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:15:35: # Command line: callpeak -t SRX859012.bam -f BAM -g dm -n SRX859012.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX859012.05 # format = BAM # ChIP-seq file = ['SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:15:35: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:15:35: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:15:43: 1000000 INFO @ Wed, 28 Jun 2017 01:15:43: 1000000 INFO @ Wed, 28 Jun 2017 01:15:44: 1000000 INFO @ Wed, 28 Jun 2017 01:15:51: 2000000 INFO @ Wed, 28 Jun 2017 01:15:51: 2000000 INFO @ Wed, 28 Jun 2017 01:15:53: 2000000 INFO @ Wed, 28 Jun 2017 01:16:00: 3000000 INFO @ Wed, 28 Jun 2017 01:16:00: 3000000 INFO @ Wed, 28 Jun 2017 01:16:02: 3000000 INFO @ Wed, 28 Jun 2017 01:16:08: 4000000 INFO @ Wed, 28 Jun 2017 01:16:08: 4000000 INFO @ Wed, 28 Jun 2017 01:16:12: 4000000 INFO @ Wed, 28 Jun 2017 01:16:16: 5000000 INFO @ Wed, 28 Jun 2017 01:16:16: 5000000 INFO @ Wed, 28 Jun 2017 01:16:21: 5000000 INFO @ Wed, 28 Jun 2017 01:16:24: 6000000 INFO @ Wed, 28 Jun 2017 01:16:24: 6000000 INFO @ Wed, 28 Jun 2017 01:16:30: 6000000 INFO @ Wed, 28 Jun 2017 01:16:33: 7000000 INFO @ Wed, 28 Jun 2017 01:16:33: 7000000 INFO @ Wed, 28 Jun 2017 01:16:39: 7000000 INFO @ Wed, 28 Jun 2017 01:16:41: 8000000 INFO @ Wed, 28 Jun 2017 01:16:41: 8000000 INFO @ Wed, 28 Jun 2017 01:16:48: 8000000 INFO @ Wed, 28 Jun 2017 01:16:49: 9000000 INFO @ Wed, 28 Jun 2017 01:16:49: 9000000 INFO @ Wed, 28 Jun 2017 01:16:57: 10000000 INFO @ Wed, 28 Jun 2017 01:16:57: 10000000 INFO @ Wed, 28 Jun 2017 01:16:57: 9000000 INFO @ Wed, 28 Jun 2017 01:17:05: 11000000 INFO @ Wed, 28 Jun 2017 01:17:05: 11000000 INFO @ Wed, 28 Jun 2017 01:17:07: 10000000 INFO @ Wed, 28 Jun 2017 01:17:14: 12000000 INFO @ Wed, 28 Jun 2017 01:17:14: 12000000 INFO @ Wed, 28 Jun 2017 01:17:16: 11000000 INFO @ Wed, 28 Jun 2017 01:17:22: 13000000 INFO @ Wed, 28 Jun 2017 01:17:22: 13000000 INFO @ Wed, 28 Jun 2017 01:17:25: 12000000 INFO @ Wed, 28 Jun 2017 01:17:29: 14000000 INFO @ Wed, 28 Jun 2017 01:17:29: 14000000 INFO @ Wed, 28 Jun 2017 01:17:33: 13000000 INFO @ Wed, 28 Jun 2017 01:17:37: 15000000 INFO @ Wed, 28 Jun 2017 01:17:37: 15000000 INFO @ Wed, 28 Jun 2017 01:17:39: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:17:39: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:17:39: #1 total tags in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:17:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:17:39: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:17:39: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:17:39: #1 total tags in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:17:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:17:40: #1 tags after filtering in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:17:40: #1 finished! INFO @ Wed, 28 Jun 2017 01:17:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:17:40: #1 tags after filtering in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:17:40: #1 finished! INFO @ Wed, 28 Jun 2017 01:17:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:17:41: #2 number of paired peaks: 2 WARNING @ Wed, 28 Jun 2017 01:17:41: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:17:41: Process for pairing-model is terminated! cat: SRX859012.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859012.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859012.05_*.xls': そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 01:17:41: #2 number of paired peaks: 2 rm: cannot remove `SRX859012.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません WARNING @ Wed, 28 Jun 2017 01:17:41: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:17:41: Process for pairing-model is terminated! CompletedMACS2peakCalling cat: SRX859012.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859012.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859012.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859012.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:17:41: 14000000 INFO @ Wed, 28 Jun 2017 01:17:48: 15000000 INFO @ Wed, 28 Jun 2017 01:17:50: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:17:50: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:17:50: #1 total tags in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:50: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:17:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:17:51: #1 tags after filtering in treatment: 15357147 INFO @ Wed, 28 Jun 2017 01:17:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:17:51: #1 finished! INFO @ Wed, 28 Jun 2017 01:17:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:17:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:17:52: #2 number of paired peaks: 2 WARNING @ Wed, 28 Jun 2017 01:17:52: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:17:52: Process for pairing-model is terminated! cat: SRX859012.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859012.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859012.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859012.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。