Job ID = 9159920 sra ファイルのダウンロード中... Completed: 223010K bytes transferred in 5 seconds (337047K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 12655696 spots for /home/okishinya/chipatlas/results/dm3/SRX858998/SRR1779554.sra Written 12655696 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:38 12655696 reads; of these: 12655696 (100.00%) were unpaired; of these: 64250 (0.51%) aligned 0 times 12118815 (95.76%) aligned exactly 1 time 472631 (3.73%) aligned >1 times 99.49% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 347 / 12591446 = 0.0000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:08:30: # Command line: callpeak -t SRX858998.bam -f BAM -g dm -n SRX858998.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX858998.10 # format = BAM # ChIP-seq file = ['SRX858998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:08:30: # Command line: callpeak -t SRX858998.bam -f BAM -g dm -n SRX858998.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX858998.20 # format = BAM # ChIP-seq file = ['SRX858998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:08:30: # Command line: callpeak -t SRX858998.bam -f BAM -g dm -n SRX858998.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX858998.05 # format = BAM # ChIP-seq file = ['SRX858998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:08:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:08:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:08:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:08:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:08:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:08:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:08:37: 1000000 INFO @ Wed, 28 Jun 2017 01:08:37: 1000000 INFO @ Wed, 28 Jun 2017 01:08:37: 1000000 INFO @ Wed, 28 Jun 2017 01:08:43: 2000000 INFO @ Wed, 28 Jun 2017 01:08:43: 2000000 INFO @ Wed, 28 Jun 2017 01:08:43: 2000000 INFO @ Wed, 28 Jun 2017 01:08:51: 3000000 INFO @ Wed, 28 Jun 2017 01:08:51: 3000000 INFO @ Wed, 28 Jun 2017 01:08:51: 3000000 INFO @ Wed, 28 Jun 2017 01:08:57: 4000000 INFO @ Wed, 28 Jun 2017 01:08:58: 4000000 INFO @ Wed, 28 Jun 2017 01:08:58: 4000000 INFO @ Wed, 28 Jun 2017 01:09:04: 5000000 INFO @ Wed, 28 Jun 2017 01:09:05: 5000000 INFO @ Wed, 28 Jun 2017 01:09:05: 5000000 INFO @ Wed, 28 Jun 2017 01:09:12: 6000000 INFO @ Wed, 28 Jun 2017 01:09:12: 6000000 INFO @ Wed, 28 Jun 2017 01:09:12: 6000000 INFO @ Wed, 28 Jun 2017 01:09:19: 7000000 INFO @ Wed, 28 Jun 2017 01:09:19: 7000000 INFO @ Wed, 28 Jun 2017 01:09:19: 7000000 INFO @ Wed, 28 Jun 2017 01:09:26: 8000000 INFO @ Wed, 28 Jun 2017 01:09:26: 8000000 INFO @ Wed, 28 Jun 2017 01:09:27: 8000000 INFO @ Wed, 28 Jun 2017 01:09:33: 9000000 INFO @ Wed, 28 Jun 2017 01:09:34: 9000000 INFO @ Wed, 28 Jun 2017 01:09:34: 9000000 INFO @ Wed, 28 Jun 2017 01:09:40: 10000000 INFO @ Wed, 28 Jun 2017 01:09:41: 10000000 INFO @ Wed, 28 Jun 2017 01:09:41: 10000000 INFO @ Wed, 28 Jun 2017 01:09:48: 11000000 INFO @ Wed, 28 Jun 2017 01:09:48: 11000000 INFO @ Wed, 28 Jun 2017 01:09:49: 11000000 INFO @ Wed, 28 Jun 2017 01:09:56: 12000000 INFO @ Wed, 28 Jun 2017 01:09:56: 12000000 INFO @ Wed, 28 Jun 2017 01:09:57: 12000000 INFO @ Wed, 28 Jun 2017 01:10:00: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:10:00: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:10:00: #1 total tags in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:00: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:10:00: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:10:00: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:10:00: #1 total tags in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:00: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:10:00: #1 tags after filtering in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:10:00: #1 finished! INFO @ Wed, 28 Jun 2017 01:10:00: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:10:00: #1 tags after filtering in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:10:00: #1 finished! INFO @ Wed, 28 Jun 2017 01:10:00: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:10:01: #2 number of paired peaks: 21 WARNING @ Wed, 28 Jun 2017 01:10:01: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:10:01: Process for pairing-model is terminated! cat: SRX858998.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX858998.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:10:01: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:10:01: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:10:01: #1 total tags in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:10:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:10:01: #2 number of paired peaks: 21 WARNING @ Wed, 28 Jun 2017 01:10:01: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:10:01: Process for pairing-model is terminated! cat: SRX858998.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX858998.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:10:01: #1 tags after filtering in treatment: 12591099 INFO @ Wed, 28 Jun 2017 01:10:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:10:01: #1 finished! INFO @ Wed, 28 Jun 2017 01:10:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:10:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:10:02: #2 number of paired peaks: 21 WARNING @ Wed, 28 Jun 2017 01:10:02: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:10:02: Process for pairing-model is terminated! cat: SRX858998.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX858998.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX858998.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。