Job ID = 14167052 SRX = SRX8574283 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 59278358 spots for SRR12045604/SRR12045604.sra Written 59278358 spots for SRR12045604/SRR12045604.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167498 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:36 59278358 reads; of these: 59278358 (100.00%) were unpaired; of these: 18588890 (31.36%) aligned 0 times 28407621 (47.92%) aligned exactly 1 time 12281847 (20.72%) aligned >1 times 68.64% overall alignment rate Time searching: 00:20:36 Overall time: 00:20:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12475056 / 40689468 = 0.3066 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:58:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:58:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:58:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:59:00: 1000000 INFO @ Fri, 10 Dec 2021 09:59:05: 2000000 INFO @ Fri, 10 Dec 2021 09:59:10: 3000000 INFO @ Fri, 10 Dec 2021 09:59:16: 4000000 INFO @ Fri, 10 Dec 2021 09:59:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:59:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:59:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:59:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:59:26: 6000000 INFO @ Fri, 10 Dec 2021 09:59:30: 1000000 INFO @ Fri, 10 Dec 2021 09:59:32: 7000000 INFO @ Fri, 10 Dec 2021 09:59:36: 2000000 INFO @ Fri, 10 Dec 2021 09:59:37: 8000000 INFO @ Fri, 10 Dec 2021 09:59:41: 3000000 INFO @ Fri, 10 Dec 2021 09:59:43: 9000000 INFO @ Fri, 10 Dec 2021 09:59:47: 4000000 INFO @ Fri, 10 Dec 2021 09:59:48: 10000000 INFO @ Fri, 10 Dec 2021 09:59:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:59:54: 11000000 INFO @ Fri, 10 Dec 2021 09:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:59:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:59:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:59:58: 6000000 INFO @ Fri, 10 Dec 2021 09:59:59: 12000000 INFO @ Fri, 10 Dec 2021 10:00:01: 1000000 INFO @ Fri, 10 Dec 2021 10:00:04: 7000000 INFO @ Fri, 10 Dec 2021 10:00:05: 13000000 INFO @ Fri, 10 Dec 2021 10:00:08: 2000000 INFO @ Fri, 10 Dec 2021 10:00:09: 8000000 INFO @ Fri, 10 Dec 2021 10:00:11: 14000000 INFO @ Fri, 10 Dec 2021 10:00:14: 3000000 INFO @ Fri, 10 Dec 2021 10:00:15: 9000000 INFO @ Fri, 10 Dec 2021 10:00:16: 15000000 INFO @ Fri, 10 Dec 2021 10:00:21: 10000000 INFO @ Fri, 10 Dec 2021 10:00:21: 4000000 INFO @ Fri, 10 Dec 2021 10:00:22: 16000000 INFO @ Fri, 10 Dec 2021 10:00:27: 11000000 INFO @ Fri, 10 Dec 2021 10:00:27: 17000000 INFO @ Fri, 10 Dec 2021 10:00:28: 5000000 INFO @ Fri, 10 Dec 2021 10:00:32: 12000000 INFO @ Fri, 10 Dec 2021 10:00:33: 18000000 INFO @ Fri, 10 Dec 2021 10:00:34: 6000000 INFO @ Fri, 10 Dec 2021 10:00:38: 13000000 INFO @ Fri, 10 Dec 2021 10:00:38: 19000000 INFO @ Fri, 10 Dec 2021 10:00:41: 7000000 INFO @ Fri, 10 Dec 2021 10:00:45: 20000000 INFO @ Fri, 10 Dec 2021 10:00:45: 14000000 INFO @ Fri, 10 Dec 2021 10:00:47: 8000000 INFO @ Fri, 10 Dec 2021 10:00:51: 21000000 INFO @ Fri, 10 Dec 2021 10:00:51: 15000000 INFO @ Fri, 10 Dec 2021 10:00:54: 9000000 INFO @ Fri, 10 Dec 2021 10:00:56: 22000000 INFO @ Fri, 10 Dec 2021 10:00:56: 16000000 INFO @ Fri, 10 Dec 2021 10:01:01: 10000000 INFO @ Fri, 10 Dec 2021 10:01:02: 23000000 INFO @ Fri, 10 Dec 2021 10:01:02: 17000000 INFO @ Fri, 10 Dec 2021 10:01:07: 11000000 INFO @ Fri, 10 Dec 2021 10:01:07: 24000000 INFO @ Fri, 10 Dec 2021 10:01:08: 18000000 INFO @ Fri, 10 Dec 2021 10:01:13: 25000000 INFO @ Fri, 10 Dec 2021 10:01:13: 19000000 INFO @ Fri, 10 Dec 2021 10:01:13: 12000000 INFO @ Fri, 10 Dec 2021 10:01:19: 26000000 INFO @ Fri, 10 Dec 2021 10:01:19: 20000000 INFO @ Fri, 10 Dec 2021 10:01:20: 13000000 INFO @ Fri, 10 Dec 2021 10:01:24: 27000000 INFO @ Fri, 10 Dec 2021 10:01:25: 21000000 INFO @ Fri, 10 Dec 2021 10:01:26: 14000000 INFO @ Fri, 10 Dec 2021 10:01:30: 28000000 INFO @ Fri, 10 Dec 2021 10:01:30: 22000000 INFO @ Fri, 10 Dec 2021 10:01:31: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:01:31: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:01:31: #1 total tags in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:01:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:01:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:01:32: #1 tags after filtering in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:01:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:01:32: #1 finished! INFO @ Fri, 10 Dec 2021 10:01:32: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:01:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:01:33: 15000000 INFO @ Fri, 10 Dec 2021 10:01:33: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:01:33: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:01:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:01:36: 23000000 INFO @ Fri, 10 Dec 2021 10:01:39: 16000000 INFO @ Fri, 10 Dec 2021 10:01:42: 24000000 INFO @ Fri, 10 Dec 2021 10:01:45: 17000000 INFO @ Fri, 10 Dec 2021 10:01:47: 25000000 INFO @ Fri, 10 Dec 2021 10:01:52: 18000000 INFO @ Fri, 10 Dec 2021 10:01:53: 26000000 INFO @ Fri, 10 Dec 2021 10:01:58: 19000000 INFO @ Fri, 10 Dec 2021 10:01:59: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:02:04: 20000000 INFO @ Fri, 10 Dec 2021 10:02:04: 28000000 INFO @ Fri, 10 Dec 2021 10:02:06: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:02:06: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:02:06: #1 total tags in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:02:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:02:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:02:06: #1 tags after filtering in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:02:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:02:06: #1 finished! INFO @ Fri, 10 Dec 2021 10:02:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:02:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:02:08: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:02:08: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:02:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:02:11: 21000000 INFO @ Fri, 10 Dec 2021 10:02:17: 22000000 INFO @ Fri, 10 Dec 2021 10:02:23: 23000000 INFO @ Fri, 10 Dec 2021 10:02:29: 24000000 INFO @ Fri, 10 Dec 2021 10:02:35: 25000000 INFO @ Fri, 10 Dec 2021 10:02:41: 26000000 INFO @ Fri, 10 Dec 2021 10:02:47: 27000000 INFO @ Fri, 10 Dec 2021 10:02:54: 28000000 INFO @ Fri, 10 Dec 2021 10:02:55: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:02:55: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:02:55: #1 total tags in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:02:55: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:02:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:02:56: #1 tags after filtering in treatment: 28214412 INFO @ Fri, 10 Dec 2021 10:02:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:02:56: #1 finished! INFO @ Fri, 10 Dec 2021 10:02:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:02:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:02:58: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:02:58: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:02:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574283/SRX8574283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。