Job ID = 14167051 SRX = SRX8574282 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 66334233 spots for SRR12045603/SRR12045603.sra Written 66334233 spots for SRR12045603/SRR12045603.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167508 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:22 66334233 reads; of these: 66334233 (100.00%) were unpaired; of these: 6661614 (10.04%) aligned 0 times 39640968 (59.76%) aligned exactly 1 time 20031651 (30.20%) aligned >1 times 89.96% overall alignment rate Time searching: 00:26:22 Overall time: 00:26:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 12666532 / 59672619 = 0.2123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:09:11: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:09:11: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:09:16: 1000000 INFO @ Fri, 10 Dec 2021 10:09:22: 2000000 INFO @ Fri, 10 Dec 2021 10:09:28: 3000000 INFO @ Fri, 10 Dec 2021 10:09:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:09:40: 5000000 INFO @ Fri, 10 Dec 2021 10:09:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:09:40: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:09:40: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:09:46: 6000000 INFO @ Fri, 10 Dec 2021 10:09:47: 1000000 INFO @ Fri, 10 Dec 2021 10:09:53: 7000000 INFO @ Fri, 10 Dec 2021 10:09:53: 2000000 INFO @ Fri, 10 Dec 2021 10:10:00: 8000000 INFO @ Fri, 10 Dec 2021 10:10:00: 3000000 INFO @ Fri, 10 Dec 2021 10:10:07: 9000000 INFO @ Fri, 10 Dec 2021 10:10:07: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:10:10: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:10:10: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:10:13: 10000000 INFO @ Fri, 10 Dec 2021 10:10:14: 5000000 INFO @ Fri, 10 Dec 2021 10:10:18: 1000000 INFO @ Fri, 10 Dec 2021 10:10:21: 11000000 INFO @ Fri, 10 Dec 2021 10:10:21: 6000000 INFO @ Fri, 10 Dec 2021 10:10:26: 2000000 INFO @ Fri, 10 Dec 2021 10:10:28: 12000000 INFO @ Fri, 10 Dec 2021 10:10:28: 7000000 INFO @ Fri, 10 Dec 2021 10:10:34: 3000000 INFO @ Fri, 10 Dec 2021 10:10:35: 13000000 INFO @ Fri, 10 Dec 2021 10:10:35: 8000000 INFO @ Fri, 10 Dec 2021 10:10:42: 4000000 INFO @ Fri, 10 Dec 2021 10:10:43: 9000000 INFO @ Fri, 10 Dec 2021 10:10:43: 14000000 INFO @ Fri, 10 Dec 2021 10:10:49: 5000000 INFO @ Fri, 10 Dec 2021 10:10:50: 15000000 INFO @ Fri, 10 Dec 2021 10:10:50: 10000000 INFO @ Fri, 10 Dec 2021 10:10:57: 6000000 INFO @ Fri, 10 Dec 2021 10:10:57: 16000000 INFO @ Fri, 10 Dec 2021 10:10:57: 11000000 INFO @ Fri, 10 Dec 2021 10:11:05: 17000000 INFO @ Fri, 10 Dec 2021 10:11:05: 12000000 INFO @ Fri, 10 Dec 2021 10:11:05: 7000000 INFO @ Fri, 10 Dec 2021 10:11:12: 18000000 INFO @ Fri, 10 Dec 2021 10:11:12: 13000000 INFO @ Fri, 10 Dec 2021 10:11:13: 8000000 INFO @ Fri, 10 Dec 2021 10:11:20: 19000000 INFO @ Fri, 10 Dec 2021 10:11:20: 14000000 INFO @ Fri, 10 Dec 2021 10:11:21: 9000000 INFO @ Fri, 10 Dec 2021 10:11:27: 20000000 INFO @ Fri, 10 Dec 2021 10:11:27: 15000000 INFO @ Fri, 10 Dec 2021 10:11:29: 10000000 INFO @ Fri, 10 Dec 2021 10:11:34: 16000000 INFO @ Fri, 10 Dec 2021 10:11:34: 21000000 INFO @ Fri, 10 Dec 2021 10:11:37: 11000000 INFO @ Fri, 10 Dec 2021 10:11:42: 17000000 INFO @ Fri, 10 Dec 2021 10:11:42: 22000000 INFO @ Fri, 10 Dec 2021 10:11:45: 12000000 INFO @ Fri, 10 Dec 2021 10:11:49: 18000000 INFO @ Fri, 10 Dec 2021 10:11:49: 23000000 INFO @ Fri, 10 Dec 2021 10:11:53: 13000000 INFO @ Fri, 10 Dec 2021 10:11:56: 19000000 INFO @ Fri, 10 Dec 2021 10:11:56: 24000000 INFO @ Fri, 10 Dec 2021 10:12:01: 14000000 INFO @ Fri, 10 Dec 2021 10:12:04: 20000000 INFO @ Fri, 10 Dec 2021 10:12:04: 25000000 INFO @ Fri, 10 Dec 2021 10:12:09: 15000000 INFO @ Fri, 10 Dec 2021 10:12:11: 21000000 INFO @ Fri, 10 Dec 2021 10:12:11: 26000000 INFO @ Fri, 10 Dec 2021 10:12:17: 16000000 INFO @ Fri, 10 Dec 2021 10:12:18: 27000000 INFO @ Fri, 10 Dec 2021 10:12:18: 22000000 INFO @ Fri, 10 Dec 2021 10:12:25: 17000000 INFO @ Fri, 10 Dec 2021 10:12:25: 28000000 INFO @ Fri, 10 Dec 2021 10:12:25: 23000000 INFO @ Fri, 10 Dec 2021 10:12:32: 18000000 INFO @ Fri, 10 Dec 2021 10:12:33: 29000000 INFO @ Fri, 10 Dec 2021 10:12:33: 24000000 INFO @ Fri, 10 Dec 2021 10:12:40: 30000000 INFO @ Fri, 10 Dec 2021 10:12:40: 25000000 INFO @ Fri, 10 Dec 2021 10:12:41: 19000000 INFO @ Fri, 10 Dec 2021 10:12:47: 31000000 INFO @ Fri, 10 Dec 2021 10:12:47: 26000000 INFO @ Fri, 10 Dec 2021 10:12:48: 20000000 INFO @ Fri, 10 Dec 2021 10:12:55: 32000000 INFO @ Fri, 10 Dec 2021 10:12:55: 27000000 INFO @ Fri, 10 Dec 2021 10:12:56: 21000000 INFO @ Fri, 10 Dec 2021 10:13:02: 28000000 INFO @ Fri, 10 Dec 2021 10:13:02: 33000000 INFO @ Fri, 10 Dec 2021 10:13:04: 22000000 INFO @ Fri, 10 Dec 2021 10:13:09: 29000000 INFO @ Fri, 10 Dec 2021 10:13:09: 34000000 INFO @ Fri, 10 Dec 2021 10:13:12: 23000000 INFO @ Fri, 10 Dec 2021 10:13:17: 30000000 INFO @ Fri, 10 Dec 2021 10:13:17: 35000000 INFO @ Fri, 10 Dec 2021 10:13:20: 24000000 INFO @ Fri, 10 Dec 2021 10:13:24: 31000000 INFO @ Fri, 10 Dec 2021 10:13:24: 36000000 INFO @ Fri, 10 Dec 2021 10:13:28: 25000000 INFO @ Fri, 10 Dec 2021 10:13:31: 32000000 INFO @ Fri, 10 Dec 2021 10:13:31: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:13:35: 26000000 INFO @ Fri, 10 Dec 2021 10:13:38: 33000000 INFO @ Fri, 10 Dec 2021 10:13:39: 38000000 INFO @ Fri, 10 Dec 2021 10:13:43: 27000000 INFO @ Fri, 10 Dec 2021 10:13:46: 34000000 INFO @ Fri, 10 Dec 2021 10:13:46: 39000000 INFO @ Fri, 10 Dec 2021 10:13:51: 28000000 INFO @ Fri, 10 Dec 2021 10:13:53: 35000000 INFO @ Fri, 10 Dec 2021 10:13:53: 40000000 INFO @ Fri, 10 Dec 2021 10:13:58: 29000000 INFO @ Fri, 10 Dec 2021 10:14:00: 36000000 INFO @ Fri, 10 Dec 2021 10:14:01: 41000000 INFO @ Fri, 10 Dec 2021 10:14:06: 30000000 INFO @ Fri, 10 Dec 2021 10:14:08: 37000000 INFO @ Fri, 10 Dec 2021 10:14:08: 42000000 INFO @ Fri, 10 Dec 2021 10:14:14: 31000000 INFO @ Fri, 10 Dec 2021 10:14:15: 38000000 INFO @ Fri, 10 Dec 2021 10:14:15: 43000000 INFO @ Fri, 10 Dec 2021 10:14:22: 32000000 INFO @ Fri, 10 Dec 2021 10:14:22: 39000000 INFO @ Fri, 10 Dec 2021 10:14:22: 44000000 INFO @ Fri, 10 Dec 2021 10:14:29: 33000000 INFO @ Fri, 10 Dec 2021 10:14:29: 40000000 INFO @ Fri, 10 Dec 2021 10:14:30: 45000000 INFO @ Fri, 10 Dec 2021 10:14:37: 41000000 INFO @ Fri, 10 Dec 2021 10:14:37: 46000000 INFO @ Fri, 10 Dec 2021 10:14:37: 34000000 INFO @ Fri, 10 Dec 2021 10:14:44: 47000000 INFO @ Fri, 10 Dec 2021 10:14:44: 42000000 INFO @ Fri, 10 Dec 2021 10:14:44: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:14:44: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:14:44: #1 total tags in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:14:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:14:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:14:45: 35000000 INFO @ Fri, 10 Dec 2021 10:14:45: #1 tags after filtering in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:14:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:14:45: #1 finished! INFO @ Fri, 10 Dec 2021 10:14:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:14:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:14:48: #2 number of paired peaks: 14 WARNING @ Fri, 10 Dec 2021 10:14:48: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:14:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:14:51: 43000000 INFO @ Fri, 10 Dec 2021 10:14:53: 36000000 INFO @ Fri, 10 Dec 2021 10:14:58: 44000000 INFO @ Fri, 10 Dec 2021 10:15:00: 37000000 INFO @ Fri, 10 Dec 2021 10:15:05: 45000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:15:08: 38000000 INFO @ Fri, 10 Dec 2021 10:15:12: 46000000 INFO @ Fri, 10 Dec 2021 10:15:16: 39000000 INFO @ Fri, 10 Dec 2021 10:15:18: 47000000 INFO @ Fri, 10 Dec 2021 10:15:19: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:15:19: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:15:19: #1 total tags in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:15:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:15:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:15:19: #1 tags after filtering in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:15:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:15:19: #1 finished! INFO @ Fri, 10 Dec 2021 10:15:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:15:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:15:22: #2 number of paired peaks: 14 WARNING @ Fri, 10 Dec 2021 10:15:22: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:15:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:15:23: 40000000 INFO @ Fri, 10 Dec 2021 10:15:31: 41000000 INFO @ Fri, 10 Dec 2021 10:15:38: 42000000 INFO @ Fri, 10 Dec 2021 10:15:45: 43000000 INFO @ Fri, 10 Dec 2021 10:15:52: 44000000 INFO @ Fri, 10 Dec 2021 10:15:59: 45000000 INFO @ Fri, 10 Dec 2021 10:16:07: 46000000 INFO @ Fri, 10 Dec 2021 10:16:13: 47000000 INFO @ Fri, 10 Dec 2021 10:16:14: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:16:14: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:16:14: #1 total tags in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:16:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:16:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:16:15: #1 tags after filtering in treatment: 47006087 INFO @ Fri, 10 Dec 2021 10:16:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:16:15: #1 finished! INFO @ Fri, 10 Dec 2021 10:16:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:16:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:16:18: #2 number of paired peaks: 14 WARNING @ Fri, 10 Dec 2021 10:16:18: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:16:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574282/SRX8574282.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling