Job ID = 14167050 SRX = SRX8574281 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 54830823 spots for SRR12045602/SRR12045602.sra Written 54830823 spots for SRR12045602/SRR12045602.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167481 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:24 54830823 reads; of these: 54830823 (100.00%) were unpaired; of these: 23820231 (43.44%) aligned 0 times 22396113 (40.85%) aligned exactly 1 time 8614479 (15.71%) aligned >1 times 56.56% overall alignment rate Time searching: 00:17:24 Overall time: 00:17:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7315458 / 31010592 = 0.2359 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:52:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:52:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:52:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:52:55: 1000000 INFO @ Fri, 10 Dec 2021 09:53:01: 2000000 INFO @ Fri, 10 Dec 2021 09:53:06: 3000000 INFO @ Fri, 10 Dec 2021 09:53:12: 4000000 INFO @ Fri, 10 Dec 2021 09:53:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:53:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:53:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:53:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:53:23: 6000000 INFO @ Fri, 10 Dec 2021 09:53:28: 1000000 INFO @ Fri, 10 Dec 2021 09:53:29: 7000000 INFO @ Fri, 10 Dec 2021 09:53:35: 8000000 INFO @ Fri, 10 Dec 2021 09:53:36: 2000000 INFO @ Fri, 10 Dec 2021 09:53:41: 9000000 INFO @ Fri, 10 Dec 2021 09:53:44: 3000000 INFO @ Fri, 10 Dec 2021 09:53:47: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:53:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:53:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:53:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:53:52: 4000000 INFO @ Fri, 10 Dec 2021 09:53:53: 11000000 INFO @ Fri, 10 Dec 2021 09:53:59: 1000000 INFO @ Fri, 10 Dec 2021 09:54:00: 12000000 INFO @ Fri, 10 Dec 2021 09:54:00: 5000000 INFO @ Fri, 10 Dec 2021 09:54:06: 13000000 INFO @ Fri, 10 Dec 2021 09:54:07: 2000000 INFO @ Fri, 10 Dec 2021 09:54:09: 6000000 INFO @ Fri, 10 Dec 2021 09:54:12: 14000000 INFO @ Fri, 10 Dec 2021 09:54:14: 3000000 INFO @ Fri, 10 Dec 2021 09:54:17: 7000000 INFO @ Fri, 10 Dec 2021 09:54:19: 15000000 INFO @ Fri, 10 Dec 2021 09:54:20: 4000000 INFO @ Fri, 10 Dec 2021 09:54:24: 8000000 INFO @ Fri, 10 Dec 2021 09:54:25: 16000000 INFO @ Fri, 10 Dec 2021 09:54:27: 5000000 INFO @ Fri, 10 Dec 2021 09:54:31: 17000000 INFO @ Fri, 10 Dec 2021 09:54:33: 9000000 INFO @ Fri, 10 Dec 2021 09:54:34: 6000000 INFO @ Fri, 10 Dec 2021 09:54:38: 18000000 INFO @ Fri, 10 Dec 2021 09:54:41: 7000000 INFO @ Fri, 10 Dec 2021 09:54:41: 10000000 INFO @ Fri, 10 Dec 2021 09:54:44: 19000000 INFO @ Fri, 10 Dec 2021 09:54:48: 8000000 INFO @ Fri, 10 Dec 2021 09:54:49: 11000000 INFO @ Fri, 10 Dec 2021 09:54:50: 20000000 INFO @ Fri, 10 Dec 2021 09:54:54: 9000000 INFO @ Fri, 10 Dec 2021 09:54:56: 21000000 INFO @ Fri, 10 Dec 2021 09:54:57: 12000000 INFO @ Fri, 10 Dec 2021 09:55:00: 10000000 INFO @ Fri, 10 Dec 2021 09:55:03: 22000000 INFO @ Fri, 10 Dec 2021 09:55:05: 13000000 INFO @ Fri, 10 Dec 2021 09:55:06: 11000000 INFO @ Fri, 10 Dec 2021 09:55:09: 23000000 INFO @ Fri, 10 Dec 2021 09:55:12: 12000000 INFO @ Fri, 10 Dec 2021 09:55:13: 14000000 INFO @ Fri, 10 Dec 2021 09:55:13: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 09:55:13: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 09:55:13: #1 total tags in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:55:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:55:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:55:13: #1 tags after filtering in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:55:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:55:13: #1 finished! INFO @ Fri, 10 Dec 2021 09:55:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:55:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:55:15: #2 number of paired peaks: 86 WARNING @ Fri, 10 Dec 2021 09:55:15: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:55:19: 13000000 INFO @ Fri, 10 Dec 2021 09:55:20: 15000000 INFO @ Fri, 10 Dec 2021 09:55:24: 14000000 INFO @ Fri, 10 Dec 2021 09:55:28: 16000000 INFO @ Fri, 10 Dec 2021 09:55:30: 15000000 INFO @ Fri, 10 Dec 2021 09:55:36: 17000000 INFO @ Fri, 10 Dec 2021 09:55:36: 16000000 INFO @ Fri, 10 Dec 2021 09:55:42: 17000000 INFO @ Fri, 10 Dec 2021 09:55:44: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:55:48: 18000000 INFO @ Fri, 10 Dec 2021 09:55:52: 19000000 INFO @ Fri, 10 Dec 2021 09:55:54: 19000000 INFO @ Fri, 10 Dec 2021 09:55:59: 20000000 INFO @ Fri, 10 Dec 2021 09:56:00: 20000000 INFO @ Fri, 10 Dec 2021 09:56:07: 21000000 INFO @ Fri, 10 Dec 2021 09:56:08: 21000000 INFO @ Fri, 10 Dec 2021 09:56:13: 22000000 INFO @ Fri, 10 Dec 2021 09:56:16: 22000000 INFO @ Fri, 10 Dec 2021 09:56:19: 23000000 INFO @ Fri, 10 Dec 2021 09:56:22: 23000000 INFO @ Fri, 10 Dec 2021 09:56:23: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 09:56:23: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 09:56:23: #1 total tags in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:56:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:56:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:56:24: #1 tags after filtering in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:56:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:56:24: #1 finished! INFO @ Fri, 10 Dec 2021 09:56:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:56:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:56:25: #2 number of paired peaks: 86 WARNING @ Fri, 10 Dec 2021 09:56:25: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:56:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:56:27: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 09:56:27: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 09:56:27: #1 total tags in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:56:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:56:27: #1 tags after filtering in treatment: 23695134 INFO @ Fri, 10 Dec 2021 09:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:56:27: #1 finished! INFO @ Fri, 10 Dec 2021 09:56:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:56:29: #2 number of paired peaks: 86 WARNING @ Fri, 10 Dec 2021 09:56:29: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:56:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574281/SRX8574281.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。