Job ID = 14167045 SRX = SRX8574276 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 62701682 spots for SRR12045597/SRR12045597.sra Written 62701682 spots for SRR12045597/SRR12045597.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167505 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:38 62701682 reads; of these: 62701682 (100.00%) were unpaired; of these: 3181394 (5.07%) aligned 0 times 41279549 (65.83%) aligned exactly 1 time 18240739 (29.09%) aligned >1 times 94.93% overall alignment rate Time searching: 00:31:39 Overall time: 00:31:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 10965952 / 59520288 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:15:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:15:33: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:15:33: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:15:42: 1000000 INFO @ Fri, 10 Dec 2021 10:15:51: 2000000 INFO @ Fri, 10 Dec 2021 10:15:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:16:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:16:03: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:16:03: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:16:08: 4000000 INFO @ Fri, 10 Dec 2021 10:16:12: 1000000 INFO @ Fri, 10 Dec 2021 10:16:18: 5000000 INFO @ Fri, 10 Dec 2021 10:16:22: 2000000 INFO @ Fri, 10 Dec 2021 10:16:27: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:16:31: 3000000 INFO @ Fri, 10 Dec 2021 10:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:16:33: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:16:33: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:16:37: 7000000 INFO @ Fri, 10 Dec 2021 10:16:41: 4000000 INFO @ Fri, 10 Dec 2021 10:16:43: 1000000 INFO @ Fri, 10 Dec 2021 10:16:47: 8000000 INFO @ Fri, 10 Dec 2021 10:16:51: 5000000 INFO @ Fri, 10 Dec 2021 10:16:53: 2000000 INFO @ Fri, 10 Dec 2021 10:16:57: 9000000 INFO @ Fri, 10 Dec 2021 10:17:01: 6000000 INFO @ Fri, 10 Dec 2021 10:17:03: 3000000 INFO @ Fri, 10 Dec 2021 10:17:07: 10000000 INFO @ Fri, 10 Dec 2021 10:17:11: 7000000 INFO @ Fri, 10 Dec 2021 10:17:14: 4000000 INFO @ Fri, 10 Dec 2021 10:17:18: 11000000 INFO @ Fri, 10 Dec 2021 10:17:21: 8000000 INFO @ Fri, 10 Dec 2021 10:17:24: 5000000 INFO @ Fri, 10 Dec 2021 10:17:28: 12000000 INFO @ Fri, 10 Dec 2021 10:17:32: 9000000 INFO @ Fri, 10 Dec 2021 10:17:34: 6000000 INFO @ Fri, 10 Dec 2021 10:17:39: 13000000 INFO @ Fri, 10 Dec 2021 10:17:41: 10000000 INFO @ Fri, 10 Dec 2021 10:17:45: 7000000 INFO @ Fri, 10 Dec 2021 10:17:50: 14000000 INFO @ Fri, 10 Dec 2021 10:17:50: 11000000 INFO @ Fri, 10 Dec 2021 10:17:55: 8000000 INFO @ Fri, 10 Dec 2021 10:18:00: 15000000 INFO @ Fri, 10 Dec 2021 10:18:01: 12000000 INFO @ Fri, 10 Dec 2021 10:18:05: 9000000 INFO @ Fri, 10 Dec 2021 10:18:10: 13000000 INFO @ Fri, 10 Dec 2021 10:18:11: 16000000 INFO @ Fri, 10 Dec 2021 10:18:15: 10000000 INFO @ Fri, 10 Dec 2021 10:18:19: 14000000 INFO @ Fri, 10 Dec 2021 10:18:20: 17000000 INFO @ Fri, 10 Dec 2021 10:18:25: 11000000 INFO @ Fri, 10 Dec 2021 10:18:29: 15000000 INFO @ Fri, 10 Dec 2021 10:18:31: 18000000 INFO @ Fri, 10 Dec 2021 10:18:35: 12000000 INFO @ Fri, 10 Dec 2021 10:18:39: 16000000 INFO @ Fri, 10 Dec 2021 10:18:40: 19000000 INFO @ Fri, 10 Dec 2021 10:18:45: 13000000 INFO @ Fri, 10 Dec 2021 10:18:48: 17000000 INFO @ Fri, 10 Dec 2021 10:18:50: 20000000 INFO @ Fri, 10 Dec 2021 10:18:55: 14000000 INFO @ Fri, 10 Dec 2021 10:18:58: 18000000 INFO @ Fri, 10 Dec 2021 10:19:00: 21000000 INFO @ Fri, 10 Dec 2021 10:19:05: 15000000 INFO @ Fri, 10 Dec 2021 10:19:07: 19000000 INFO @ Fri, 10 Dec 2021 10:19:10: 22000000 INFO @ Fri, 10 Dec 2021 10:19:15: 16000000 INFO @ Fri, 10 Dec 2021 10:19:16: 20000000 INFO @ Fri, 10 Dec 2021 10:19:20: 23000000 INFO @ Fri, 10 Dec 2021 10:19:25: 17000000 INFO @ Fri, 10 Dec 2021 10:19:26: 21000000 INFO @ Fri, 10 Dec 2021 10:19:30: 24000000 INFO @ Fri, 10 Dec 2021 10:19:35: 18000000 INFO @ Fri, 10 Dec 2021 10:19:35: 22000000 INFO @ Fri, 10 Dec 2021 10:19:40: 25000000 INFO @ Fri, 10 Dec 2021 10:19:45: 23000000 INFO @ Fri, 10 Dec 2021 10:19:45: 19000000 INFO @ Fri, 10 Dec 2021 10:19:50: 26000000 INFO @ Fri, 10 Dec 2021 10:19:55: 24000000 INFO @ Fri, 10 Dec 2021 10:19:56: 20000000 INFO @ Fri, 10 Dec 2021 10:20:00: 27000000 INFO @ Fri, 10 Dec 2021 10:20:05: 25000000 INFO @ Fri, 10 Dec 2021 10:20:06: 21000000 INFO @ Fri, 10 Dec 2021 10:20:11: 28000000 INFO @ Fri, 10 Dec 2021 10:20:15: 26000000 INFO @ Fri, 10 Dec 2021 10:20:16: 22000000 INFO @ Fri, 10 Dec 2021 10:20:21: 29000000 INFO @ Fri, 10 Dec 2021 10:20:25: 27000000 INFO @ Fri, 10 Dec 2021 10:20:26: 23000000 INFO @ Fri, 10 Dec 2021 10:20:31: 30000000 INFO @ Fri, 10 Dec 2021 10:20:35: 28000000 INFO @ Fri, 10 Dec 2021 10:20:36: 24000000 INFO @ Fri, 10 Dec 2021 10:20:41: 31000000 INFO @ Fri, 10 Dec 2021 10:20:45: 29000000 INFO @ Fri, 10 Dec 2021 10:20:47: 25000000 INFO @ Fri, 10 Dec 2021 10:20:50: 32000000 INFO @ Fri, 10 Dec 2021 10:20:56: 30000000 INFO @ Fri, 10 Dec 2021 10:20:57: 26000000 INFO @ Fri, 10 Dec 2021 10:21:02: 33000000 INFO @ Fri, 10 Dec 2021 10:21:05: 31000000 INFO @ Fri, 10 Dec 2021 10:21:07: 27000000 INFO @ Fri, 10 Dec 2021 10:21:12: 34000000 INFO @ Fri, 10 Dec 2021 10:21:15: 32000000 INFO @ Fri, 10 Dec 2021 10:21:18: 28000000 INFO @ Fri, 10 Dec 2021 10:21:22: 35000000 INFO @ Fri, 10 Dec 2021 10:21:25: 33000000 INFO @ Fri, 10 Dec 2021 10:21:29: 29000000 INFO @ Fri, 10 Dec 2021 10:21:33: 36000000 INFO @ Fri, 10 Dec 2021 10:21:35: 34000000 INFO @ Fri, 10 Dec 2021 10:21:39: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:21:43: 37000000 INFO @ Fri, 10 Dec 2021 10:21:44: 35000000 INFO @ Fri, 10 Dec 2021 10:21:49: 31000000 INFO @ Fri, 10 Dec 2021 10:21:53: 38000000 INFO @ Fri, 10 Dec 2021 10:21:54: 36000000 INFO @ Fri, 10 Dec 2021 10:21:58: 32000000 INFO @ Fri, 10 Dec 2021 10:22:03: 39000000 INFO @ Fri, 10 Dec 2021 10:22:04: 37000000 INFO @ Fri, 10 Dec 2021 10:22:08: 33000000 INFO @ Fri, 10 Dec 2021 10:22:13: 40000000 INFO @ Fri, 10 Dec 2021 10:22:14: 38000000 INFO @ Fri, 10 Dec 2021 10:22:18: 34000000 INFO @ Fri, 10 Dec 2021 10:22:23: 39000000 INFO @ Fri, 10 Dec 2021 10:22:24: 41000000 INFO @ Fri, 10 Dec 2021 10:22:28: 35000000 INFO @ Fri, 10 Dec 2021 10:22:33: 40000000 INFO @ Fri, 10 Dec 2021 10:22:34: 42000000 INFO @ Fri, 10 Dec 2021 10:22:39: 36000000 INFO @ Fri, 10 Dec 2021 10:22:43: 41000000 INFO @ Fri, 10 Dec 2021 10:22:46: 43000000 INFO @ Fri, 10 Dec 2021 10:22:49: 37000000 INFO @ Fri, 10 Dec 2021 10:22:52: 42000000 INFO @ Fri, 10 Dec 2021 10:22:56: 44000000 INFO @ Fri, 10 Dec 2021 10:22:59: 38000000 INFO @ Fri, 10 Dec 2021 10:23:01: 43000000 INFO @ Fri, 10 Dec 2021 10:23:06: 45000000 INFO @ Fri, 10 Dec 2021 10:23:09: 39000000 INFO @ Fri, 10 Dec 2021 10:23:12: 44000000 INFO @ Fri, 10 Dec 2021 10:23:16: 46000000 INFO @ Fri, 10 Dec 2021 10:23:19: 40000000 INFO @ Fri, 10 Dec 2021 10:23:21: 45000000 INFO @ Fri, 10 Dec 2021 10:23:26: 47000000 INFO @ Fri, 10 Dec 2021 10:23:29: 41000000 INFO @ Fri, 10 Dec 2021 10:23:30: 46000000 INFO @ Fri, 10 Dec 2021 10:23:37: 48000000 INFO @ Fri, 10 Dec 2021 10:23:40: 42000000 INFO @ Fri, 10 Dec 2021 10:23:41: 47000000 INFO @ Fri, 10 Dec 2021 10:23:43: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:23:43: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:23:43: #1 total tags in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:23:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:23:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:23:44: #1 tags after filtering in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:23:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:23:44: #1 finished! INFO @ Fri, 10 Dec 2021 10:23:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:23:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:23:47: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:23:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:23:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:23:49: 43000000 INFO @ Fri, 10 Dec 2021 10:23:50: 48000000 INFO @ Fri, 10 Dec 2021 10:23:55: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:23:55: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:23:55: #1 total tags in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:23:55: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:23:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:23:56: #1 tags after filtering in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:23:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:23:56: #1 finished! INFO @ Fri, 10 Dec 2021 10:23:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:23:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:23:58: 44000000 INFO @ Fri, 10 Dec 2021 10:23:59: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:23:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:23:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:24:07: 45000000 INFO @ Fri, 10 Dec 2021 10:24:15: 46000000 INFO @ Fri, 10 Dec 2021 10:24:24: 47000000 INFO @ Fri, 10 Dec 2021 10:24:32: 48000000 INFO @ Fri, 10 Dec 2021 10:24:37: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:24:37: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:24:37: #1 total tags in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:24:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:24:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:24:38: #1 tags after filtering in treatment: 48554336 INFO @ Fri, 10 Dec 2021 10:24:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:24:38: #1 finished! INFO @ Fri, 10 Dec 2021 10:24:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:24:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:24:42: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:24:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:24:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574276/SRX8574276.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling