Job ID = 14167044 SRX = SRX8574271 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30783256 spots for SRR12045592/SRR12045592.sra Written 30783256 spots for SRR12045592/SRR12045592.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167413 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:38 30783256 reads; of these: 30783256 (100.00%) were unpaired; of these: 3139648 (10.20%) aligned 0 times 18783120 (61.02%) aligned exactly 1 time 8860488 (28.78%) aligned >1 times 89.80% overall alignment rate Time searching: 00:09:38 Overall time: 00:09:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5092909 / 27643608 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:29:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:29:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:29:51: 1000000 INFO @ Fri, 10 Dec 2021 09:29:56: 2000000 INFO @ Fri, 10 Dec 2021 09:30:00: 3000000 INFO @ Fri, 10 Dec 2021 09:30:05: 4000000 INFO @ Fri, 10 Dec 2021 09:30:09: 5000000 INFO @ Fri, 10 Dec 2021 09:30:13: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:30:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:30:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:30:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:30:18: 7000000 INFO @ Fri, 10 Dec 2021 09:30:21: 1000000 INFO @ Fri, 10 Dec 2021 09:30:22: 8000000 INFO @ Fri, 10 Dec 2021 09:30:26: 2000000 INFO @ Fri, 10 Dec 2021 09:30:26: 9000000 INFO @ Fri, 10 Dec 2021 09:30:30: 3000000 INFO @ Fri, 10 Dec 2021 09:30:31: 10000000 INFO @ Fri, 10 Dec 2021 09:30:35: 4000000 INFO @ Fri, 10 Dec 2021 09:30:35: 11000000 INFO @ Fri, 10 Dec 2021 09:30:39: 5000000 INFO @ Fri, 10 Dec 2021 09:30:40: 12000000 INFO @ Fri, 10 Dec 2021 09:30:44: 6000000 INFO @ Fri, 10 Dec 2021 09:30:44: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:30:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:30:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:30:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:30:48: 7000000 INFO @ Fri, 10 Dec 2021 09:30:48: 14000000 INFO @ Fri, 10 Dec 2021 09:30:52: 1000000 INFO @ Fri, 10 Dec 2021 09:30:52: 8000000 INFO @ Fri, 10 Dec 2021 09:30:53: 15000000 INFO @ Fri, 10 Dec 2021 09:30:56: 2000000 INFO @ Fri, 10 Dec 2021 09:30:57: 9000000 INFO @ Fri, 10 Dec 2021 09:30:57: 16000000 INFO @ Fri, 10 Dec 2021 09:31:00: 3000000 INFO @ Fri, 10 Dec 2021 09:31:01: 10000000 INFO @ Fri, 10 Dec 2021 09:31:02: 17000000 INFO @ Fri, 10 Dec 2021 09:31:05: 4000000 INFO @ Fri, 10 Dec 2021 09:31:06: 11000000 INFO @ Fri, 10 Dec 2021 09:31:06: 18000000 INFO @ Fri, 10 Dec 2021 09:31:09: 5000000 INFO @ Fri, 10 Dec 2021 09:31:10: 12000000 INFO @ Fri, 10 Dec 2021 09:31:11: 19000000 INFO @ Fri, 10 Dec 2021 09:31:14: 6000000 INFO @ Fri, 10 Dec 2021 09:31:14: 13000000 INFO @ Fri, 10 Dec 2021 09:31:15: 20000000 INFO @ Fri, 10 Dec 2021 09:31:18: 7000000 INFO @ Fri, 10 Dec 2021 09:31:19: 14000000 INFO @ Fri, 10 Dec 2021 09:31:19: 21000000 INFO @ Fri, 10 Dec 2021 09:31:22: 8000000 INFO @ Fri, 10 Dec 2021 09:31:23: 15000000 INFO @ Fri, 10 Dec 2021 09:31:24: 22000000 INFO @ Fri, 10 Dec 2021 09:31:26: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:31:26: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:31:26: #1 total tags in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:31:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:31:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:31:27: #1 tags after filtering in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:31:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:31:27: #1 finished! INFO @ Fri, 10 Dec 2021 09:31:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:31:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:31:27: 9000000 INFO @ Fri, 10 Dec 2021 09:31:28: 16000000 INFO @ Fri, 10 Dec 2021 09:31:28: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 09:31:28: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:31:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:31:31: 10000000 INFO @ Fri, 10 Dec 2021 09:31:32: 17000000 INFO @ Fri, 10 Dec 2021 09:31:36: 11000000 INFO @ Fri, 10 Dec 2021 09:31:36: 18000000 INFO @ Fri, 10 Dec 2021 09:31:40: 12000000 INFO @ Fri, 10 Dec 2021 09:31:41: 19000000 INFO @ Fri, 10 Dec 2021 09:31:45: 13000000 INFO @ Fri, 10 Dec 2021 09:31:45: 20000000 INFO @ Fri, 10 Dec 2021 09:31:49: 14000000 INFO @ Fri, 10 Dec 2021 09:31:50: 21000000 INFO @ Fri, 10 Dec 2021 09:31:53: 15000000 INFO @ Fri, 10 Dec 2021 09:31:54: 22000000 INFO @ Fri, 10 Dec 2021 09:31:57: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:31:57: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:31:57: #1 total tags in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:31:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:31:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:31:57: #1 tags after filtering in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:31:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:31:57: #1 finished! INFO @ Fri, 10 Dec 2021 09:31:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:31:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:31:58: 16000000 INFO @ Fri, 10 Dec 2021 09:31:59: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 09:31:59: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:31:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:32:02: 17000000 INFO @ Fri, 10 Dec 2021 09:32:07: 18000000 INFO @ Fri, 10 Dec 2021 09:32:11: 19000000 INFO @ Fri, 10 Dec 2021 09:32:16: 20000000 INFO @ Fri, 10 Dec 2021 09:32:20: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:32:24: 22000000 INFO @ Fri, 10 Dec 2021 09:32:27: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:32:27: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:32:27: #1 total tags in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:32:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:32:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:32:27: #1 tags after filtering in treatment: 22550699 INFO @ Fri, 10 Dec 2021 09:32:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:32:27: #1 finished! INFO @ Fri, 10 Dec 2021 09:32:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:32:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:32:29: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 09:32:29: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:32:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574271/SRX8574271.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。