Job ID = 14167034 SRX = SRX8574269 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 52941533 spots for SRR12045590/SRR12045590.sra Written 52941533 spots for SRR12045590/SRR12045590.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167408 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:43 52941533 reads; of these: 52941533 (100.00%) were unpaired; of these: 14778134 (27.91%) aligned 0 times 24707528 (46.67%) aligned exactly 1 time 13455871 (25.42%) aligned >1 times 72.09% overall alignment rate Time searching: 00:14:44 Overall time: 00:14:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12940467 / 38163399 = 0.3391 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:24:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:24:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:24:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:24:26: 1000000 INFO @ Fri, 10 Dec 2021 09:24:31: 2000000 INFO @ Fri, 10 Dec 2021 09:24:37: 3000000 INFO @ Fri, 10 Dec 2021 09:24:42: 4000000 INFO @ Fri, 10 Dec 2021 09:24:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:24:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:24:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:24:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:24:53: 6000000 INFO @ Fri, 10 Dec 2021 09:24:55: 1000000 INFO @ Fri, 10 Dec 2021 09:24:59: 7000000 INFO @ Fri, 10 Dec 2021 09:25:00: 2000000 INFO @ Fri, 10 Dec 2021 09:25:04: 8000000 INFO @ Fri, 10 Dec 2021 09:25:05: 3000000 INFO @ Fri, 10 Dec 2021 09:25:09: 9000000 INFO @ Fri, 10 Dec 2021 09:25:10: 4000000 INFO @ Fri, 10 Dec 2021 09:25:14: 10000000 INFO @ Fri, 10 Dec 2021 09:25:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:25:19: 11000000 INFO @ Fri, 10 Dec 2021 09:25:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:25:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:25:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:25:21: 6000000 INFO @ Fri, 10 Dec 2021 09:25:24: 12000000 INFO @ Fri, 10 Dec 2021 09:25:26: 7000000 INFO @ Fri, 10 Dec 2021 09:25:26: 1000000 INFO @ Fri, 10 Dec 2021 09:25:29: 13000000 INFO @ Fri, 10 Dec 2021 09:25:31: 8000000 INFO @ Fri, 10 Dec 2021 09:25:32: 2000000 INFO @ Fri, 10 Dec 2021 09:25:34: 14000000 INFO @ Fri, 10 Dec 2021 09:25:36: 9000000 INFO @ Fri, 10 Dec 2021 09:25:38: 3000000 INFO @ Fri, 10 Dec 2021 09:25:39: 15000000 INFO @ Fri, 10 Dec 2021 09:25:41: 10000000 INFO @ Fri, 10 Dec 2021 09:25:43: 4000000 INFO @ Fri, 10 Dec 2021 09:25:44: 16000000 INFO @ Fri, 10 Dec 2021 09:25:46: 11000000 INFO @ Fri, 10 Dec 2021 09:25:49: 17000000 INFO @ Fri, 10 Dec 2021 09:25:49: 5000000 INFO @ Fri, 10 Dec 2021 09:25:51: 12000000 INFO @ Fri, 10 Dec 2021 09:25:54: 18000000 INFO @ Fri, 10 Dec 2021 09:25:55: 6000000 INFO @ Fri, 10 Dec 2021 09:25:56: 13000000 INFO @ Fri, 10 Dec 2021 09:25:59: 19000000 INFO @ Fri, 10 Dec 2021 09:26:00: 14000000 INFO @ Fri, 10 Dec 2021 09:26:01: 7000000 INFO @ Fri, 10 Dec 2021 09:26:04: 20000000 INFO @ Fri, 10 Dec 2021 09:26:05: 15000000 INFO @ Fri, 10 Dec 2021 09:26:07: 8000000 INFO @ Fri, 10 Dec 2021 09:26:09: 21000000 INFO @ Fri, 10 Dec 2021 09:26:10: 16000000 INFO @ Fri, 10 Dec 2021 09:26:12: 9000000 INFO @ Fri, 10 Dec 2021 09:26:13: 22000000 INFO @ Fri, 10 Dec 2021 09:26:15: 17000000 INFO @ Fri, 10 Dec 2021 09:26:18: 10000000 INFO @ Fri, 10 Dec 2021 09:26:18: 23000000 INFO @ Fri, 10 Dec 2021 09:26:20: 18000000 INFO @ Fri, 10 Dec 2021 09:26:23: 24000000 INFO @ Fri, 10 Dec 2021 09:26:24: 11000000 INFO @ Fri, 10 Dec 2021 09:26:25: 19000000 INFO @ Fri, 10 Dec 2021 09:26:28: 25000000 INFO @ Fri, 10 Dec 2021 09:26:29: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:26:29: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:26:29: #1 total tags in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:26:29: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:26:30: #1 tags after filtering in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:26:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:26:30: #1 finished! INFO @ Fri, 10 Dec 2021 09:26:30: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:26:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:26:30: 12000000 INFO @ Fri, 10 Dec 2021 09:26:30: 20000000 INFO @ Fri, 10 Dec 2021 09:26:31: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 09:26:31: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:26:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:26:35: 21000000 INFO @ Fri, 10 Dec 2021 09:26:36: 13000000 INFO @ Fri, 10 Dec 2021 09:26:40: 22000000 INFO @ Fri, 10 Dec 2021 09:26:42: 14000000 INFO @ Fri, 10 Dec 2021 09:26:45: 23000000 INFO @ Fri, 10 Dec 2021 09:26:47: 15000000 INFO @ Fri, 10 Dec 2021 09:26:49: 24000000 INFO @ Fri, 10 Dec 2021 09:26:53: 16000000 INFO @ Fri, 10 Dec 2021 09:26:54: 25000000 INFO @ Fri, 10 Dec 2021 09:26:56: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:26:56: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:26:56: #1 total tags in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:26:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:26:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:26:56: #1 tags after filtering in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:26:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:26:56: #1 finished! INFO @ Fri, 10 Dec 2021 09:26:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:26:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:26:58: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 09:26:58: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:26:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:26:59: 17000000 INFO @ Fri, 10 Dec 2021 09:27:04: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:27:10: 19000000 INFO @ Fri, 10 Dec 2021 09:27:15: 20000000 INFO @ Fri, 10 Dec 2021 09:27:21: 21000000 INFO @ Fri, 10 Dec 2021 09:27:26: 22000000 INFO @ Fri, 10 Dec 2021 09:27:31: 23000000 INFO @ Fri, 10 Dec 2021 09:27:37: 24000000 INFO @ Fri, 10 Dec 2021 09:27:42: 25000000 INFO @ Fri, 10 Dec 2021 09:27:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:27:43: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:27:43: #1 total tags in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:27:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:27:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:27:44: #1 tags after filtering in treatment: 25222932 INFO @ Fri, 10 Dec 2021 09:27:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:27:44: #1 finished! INFO @ Fri, 10 Dec 2021 09:27:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:27:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:27:45: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 09:27:45: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:27:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574269/SRX8574269.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。