Job ID = 14167033 SRX = SRX8574268 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 62882314 spots for SRR12045589/SRR12045589.sra Written 62882314 spots for SRR12045589/SRR12045589.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167446 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:49 62882314 reads; of these: 62882314 (100.00%) were unpaired; of these: 6349008 (10.10%) aligned 0 times 33997709 (54.07%) aligned exactly 1 time 22535597 (35.84%) aligned >1 times 89.90% overall alignment rate Time searching: 00:28:49 Overall time: 00:28:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 16298872 / 56533306 = 0.2883 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:44:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:44:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:44:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:44:41: 1000000 INFO @ Fri, 10 Dec 2021 09:44:46: 2000000 INFO @ Fri, 10 Dec 2021 09:44:51: 3000000 INFO @ Fri, 10 Dec 2021 09:44:56: 4000000 INFO @ Fri, 10 Dec 2021 09:45:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:45:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:45:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:45:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:45:06: 6000000 INFO @ Fri, 10 Dec 2021 09:45:11: 1000000 INFO @ Fri, 10 Dec 2021 09:45:12: 7000000 INFO @ Fri, 10 Dec 2021 09:45:17: 2000000 INFO @ Fri, 10 Dec 2021 09:45:17: 8000000 INFO @ Fri, 10 Dec 2021 09:45:22: 3000000 INFO @ Fri, 10 Dec 2021 09:45:23: 9000000 INFO @ Fri, 10 Dec 2021 09:45:28: 4000000 INFO @ Fri, 10 Dec 2021 09:45:28: 10000000 BedGraph に変換中... INFO @ Fri, 10 Dec 2021 09:45:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:45:34: 11000000 INFO @ Fri, 10 Dec 2021 09:45:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:45:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:45:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:45:39: 6000000 INFO @ Fri, 10 Dec 2021 09:45:39: 12000000 INFO @ Fri, 10 Dec 2021 09:45:41: 1000000 INFO @ Fri, 10 Dec 2021 09:45:45: 7000000 INFO @ Fri, 10 Dec 2021 09:45:45: 13000000 INFO @ Fri, 10 Dec 2021 09:45:46: 2000000 INFO @ Fri, 10 Dec 2021 09:45:50: 8000000 INFO @ Fri, 10 Dec 2021 09:45:51: 14000000 INFO @ Fri, 10 Dec 2021 09:45:52: 3000000 INFO @ Fri, 10 Dec 2021 09:45:56: 9000000 INFO @ Fri, 10 Dec 2021 09:45:56: 15000000 INFO @ Fri, 10 Dec 2021 09:45:57: 4000000 INFO @ Fri, 10 Dec 2021 09:46:01: 10000000 INFO @ Fri, 10 Dec 2021 09:46:02: 5000000 INFO @ Fri, 10 Dec 2021 09:46:02: 16000000 INFO @ Fri, 10 Dec 2021 09:46:07: 6000000 INFO @ Fri, 10 Dec 2021 09:46:07: 11000000 INFO @ Fri, 10 Dec 2021 09:46:08: 17000000 INFO @ Fri, 10 Dec 2021 09:46:12: 7000000 INFO @ Fri, 10 Dec 2021 09:46:13: 12000000 INFO @ Fri, 10 Dec 2021 09:46:13: 18000000 INFO @ Fri, 10 Dec 2021 09:46:17: 8000000 INFO @ Fri, 10 Dec 2021 09:46:19: 13000000 INFO @ Fri, 10 Dec 2021 09:46:19: 19000000 INFO @ Fri, 10 Dec 2021 09:46:22: 9000000 INFO @ Fri, 10 Dec 2021 09:46:25: 14000000 INFO @ Fri, 10 Dec 2021 09:46:25: 20000000 INFO @ Fri, 10 Dec 2021 09:46:27: 10000000 INFO @ Fri, 10 Dec 2021 09:46:30: 15000000 INFO @ Fri, 10 Dec 2021 09:46:31: 21000000 INFO @ Fri, 10 Dec 2021 09:46:33: 11000000 INFO @ Fri, 10 Dec 2021 09:46:36: 16000000 INFO @ Fri, 10 Dec 2021 09:46:37: 22000000 INFO @ Fri, 10 Dec 2021 09:46:38: 12000000 INFO @ Fri, 10 Dec 2021 09:46:42: 17000000 INFO @ Fri, 10 Dec 2021 09:46:42: 23000000 INFO @ Fri, 10 Dec 2021 09:46:43: 13000000 INFO @ Fri, 10 Dec 2021 09:46:48: 18000000 INFO @ Fri, 10 Dec 2021 09:46:48: 24000000 INFO @ Fri, 10 Dec 2021 09:46:48: 14000000 INFO @ Fri, 10 Dec 2021 09:46:53: 19000000 INFO @ Fri, 10 Dec 2021 09:46:53: 15000000 INFO @ Fri, 10 Dec 2021 09:46:54: 25000000 INFO @ Fri, 10 Dec 2021 09:46:59: 16000000 INFO @ Fri, 10 Dec 2021 09:46:59: 20000000 INFO @ Fri, 10 Dec 2021 09:47:00: 26000000 INFO @ Fri, 10 Dec 2021 09:47:04: 17000000 INFO @ Fri, 10 Dec 2021 09:47:05: 21000000 INFO @ Fri, 10 Dec 2021 09:47:06: 27000000 INFO @ Fri, 10 Dec 2021 09:47:09: 18000000 INFO @ Fri, 10 Dec 2021 09:47:11: 22000000 INFO @ Fri, 10 Dec 2021 09:47:12: 28000000 INFO @ Fri, 10 Dec 2021 09:47:14: 19000000 INFO @ Fri, 10 Dec 2021 09:47:17: 23000000 INFO @ Fri, 10 Dec 2021 09:47:17: 29000000 INFO @ Fri, 10 Dec 2021 09:47:19: 20000000 INFO @ Fri, 10 Dec 2021 09:47:23: 24000000 INFO @ Fri, 10 Dec 2021 09:47:23: 30000000 INFO @ Fri, 10 Dec 2021 09:47:24: 21000000 INFO @ Fri, 10 Dec 2021 09:47:28: 25000000 INFO @ Fri, 10 Dec 2021 09:47:29: 31000000 INFO @ Fri, 10 Dec 2021 09:47:30: 22000000 INFO @ Fri, 10 Dec 2021 09:47:34: 26000000 INFO @ Fri, 10 Dec 2021 09:47:34: 32000000 INFO @ Fri, 10 Dec 2021 09:47:35: 23000000 INFO @ Fri, 10 Dec 2021 09:47:39: 27000000 INFO @ Fri, 10 Dec 2021 09:47:40: 33000000 INFO @ Fri, 10 Dec 2021 09:47:40: 24000000 INFO @ Fri, 10 Dec 2021 09:47:45: 28000000 INFO @ Fri, 10 Dec 2021 09:47:45: 25000000 INFO @ Fri, 10 Dec 2021 09:47:46: 34000000 INFO @ Fri, 10 Dec 2021 09:47:50: 26000000 INFO @ Fri, 10 Dec 2021 09:47:51: 29000000 INFO @ Fri, 10 Dec 2021 09:47:52: 35000000 INFO @ Fri, 10 Dec 2021 09:47:56: 27000000 INFO @ Fri, 10 Dec 2021 09:47:57: 30000000 INFO @ Fri, 10 Dec 2021 09:47:57: 36000000 INFO @ Fri, 10 Dec 2021 09:48:01: 28000000 INFO @ Fri, 10 Dec 2021 09:48:02: 31000000 INFO @ Fri, 10 Dec 2021 09:48:03: 37000000 INFO @ Fri, 10 Dec 2021 09:48:06: 29000000 INFO @ Fri, 10 Dec 2021 09:48:08: 32000000 INFO @ Fri, 10 Dec 2021 09:48:09: 38000000 INFO @ Fri, 10 Dec 2021 09:48:11: 30000000 INFO @ Fri, 10 Dec 2021 09:48:14: 33000000 INFO @ Fri, 10 Dec 2021 09:48:15: 39000000 INFO @ Fri, 10 Dec 2021 09:48:17: 31000000 INFO @ Fri, 10 Dec 2021 09:48:20: 34000000 INFO @ Fri, 10 Dec 2021 09:48:21: 40000000 INFO @ Fri, 10 Dec 2021 09:48:22: 32000000 INFO @ Fri, 10 Dec 2021 09:48:22: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:48:22: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:48:22: #1 total tags in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:48:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:48:23: #1 tags after filtering in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:48:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:48:23: #1 finished! INFO @ Fri, 10 Dec 2021 09:48:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:48:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:48:25: 35000000 INFO @ Fri, 10 Dec 2021 09:48:26: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 09:48:26: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:48:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:48:27: 33000000 INFO @ Fri, 10 Dec 2021 09:48:30: 36000000 INFO @ Fri, 10 Dec 2021 09:48:32: 34000000 INFO @ Fri, 10 Dec 2021 09:48:35: 37000000 INFO @ Fri, 10 Dec 2021 09:48:37: 35000000 INFO @ Fri, 10 Dec 2021 09:48:41: 38000000 INFO @ Fri, 10 Dec 2021 09:48:42: 36000000 INFO @ Fri, 10 Dec 2021 09:48:46: 39000000 INFO @ Fri, 10 Dec 2021 09:48:48: 37000000 INFO @ Fri, 10 Dec 2021 09:48:51: 40000000 INFO @ Fri, 10 Dec 2021 09:48:53: 38000000 INFO @ Fri, 10 Dec 2021 09:48:53: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:48:53: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:48:53: #1 total tags in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:48:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:48:53: #1 tags after filtering in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:48:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:48:53: #1 finished! INFO @ Fri, 10 Dec 2021 09:48:53: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:48:56: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 09:48:56: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:48:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:48:58: 39000000 INFO @ Fri, 10 Dec 2021 09:49:03: 40000000 INFO @ Fri, 10 Dec 2021 09:49:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:49:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:49:04: #1 total tags in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:49:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:49:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:49:05: #1 tags after filtering in treatment: 40234434 INFO @ Fri, 10 Dec 2021 09:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:49:05: #1 finished! INFO @ Fri, 10 Dec 2021 09:49:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:49:07: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 09:49:07: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:49:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574268/SRX8574268.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。