Job ID = 14167025 SRX = SRX8574260 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39301965 spots for SRR12045581/SRR12045581.sra Written 39301965 spots for SRR12045581/SRR12045581.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167399 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:02 39301965 reads; of these: 39301965 (100.00%) were unpaired; of these: 2527006 (6.43%) aligned 0 times 21286751 (54.16%) aligned exactly 1 time 15488208 (39.41%) aligned >1 times 93.57% overall alignment rate Time searching: 00:17:02 Overall time: 00:17:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11448155 / 36774959 = 0.3113 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:23:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:23:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:23:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:23:57: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:24:11: 2000000 INFO @ Fri, 10 Dec 2021 09:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:24:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:24:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:24:23: 3000000 INFO @ Fri, 10 Dec 2021 09:24:33: 1000000 INFO @ Fri, 10 Dec 2021 09:24:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:24:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:24:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:24:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:24:47: 5000000 INFO @ Fri, 10 Dec 2021 09:24:50: 2000000 INFO @ Fri, 10 Dec 2021 09:24:59: 6000000 INFO @ Fri, 10 Dec 2021 09:25:04: 1000000 INFO @ Fri, 10 Dec 2021 09:25:09: 3000000 INFO @ Fri, 10 Dec 2021 09:25:10: 7000000 INFO @ Fri, 10 Dec 2021 09:25:22: 2000000 INFO @ Fri, 10 Dec 2021 09:25:22: 8000000 INFO @ Fri, 10 Dec 2021 09:25:29: 4000000 INFO @ Fri, 10 Dec 2021 09:25:35: 9000000 INFO @ Fri, 10 Dec 2021 09:25:40: 3000000 INFO @ Fri, 10 Dec 2021 09:25:48: 10000000 INFO @ Fri, 10 Dec 2021 09:25:48: 5000000 INFO @ Fri, 10 Dec 2021 09:25:56: 4000000 INFO @ Fri, 10 Dec 2021 09:26:00: 11000000 INFO @ Fri, 10 Dec 2021 09:26:07: 6000000 INFO @ Fri, 10 Dec 2021 09:26:11: 12000000 INFO @ Fri, 10 Dec 2021 09:26:13: 5000000 INFO @ Fri, 10 Dec 2021 09:26:22: 13000000 INFO @ Fri, 10 Dec 2021 09:26:26: 7000000 INFO @ Fri, 10 Dec 2021 09:26:30: 6000000 INFO @ Fri, 10 Dec 2021 09:26:33: 14000000 INFO @ Fri, 10 Dec 2021 09:26:45: 15000000 INFO @ Fri, 10 Dec 2021 09:26:46: 8000000 INFO @ Fri, 10 Dec 2021 09:26:50: 7000000 INFO @ Fri, 10 Dec 2021 09:26:58: 16000000 INFO @ Fri, 10 Dec 2021 09:27:05: 9000000 INFO @ Fri, 10 Dec 2021 09:27:10: 17000000 INFO @ Fri, 10 Dec 2021 09:27:11: 8000000 INFO @ Fri, 10 Dec 2021 09:27:21: 18000000 INFO @ Fri, 10 Dec 2021 09:27:25: 10000000 INFO @ Fri, 10 Dec 2021 09:27:28: 9000000 INFO @ Fri, 10 Dec 2021 09:27:33: 19000000 INFO @ Fri, 10 Dec 2021 09:27:43: 20000000 INFO @ Fri, 10 Dec 2021 09:27:45: 11000000 INFO @ Fri, 10 Dec 2021 09:27:46: 10000000 INFO @ Fri, 10 Dec 2021 09:27:55: 21000000 INFO @ Fri, 10 Dec 2021 09:28:03: 11000000 INFO @ Fri, 10 Dec 2021 09:28:05: 12000000 INFO @ Fri, 10 Dec 2021 09:28:09: 22000000 INFO @ Fri, 10 Dec 2021 09:28:22: 12000000 INFO @ Fri, 10 Dec 2021 09:28:22: 23000000 INFO @ Fri, 10 Dec 2021 09:28:25: 13000000 INFO @ Fri, 10 Dec 2021 09:28:35: 24000000 INFO @ Fri, 10 Dec 2021 09:28:39: 13000000 INFO @ Fri, 10 Dec 2021 09:28:43: 14000000 INFO @ Fri, 10 Dec 2021 09:28:47: 25000000 INFO @ Fri, 10 Dec 2021 09:28:51: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:28:51: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:28:51: #1 total tags in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:28:51: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:28:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:28:51: #1 tags after filtering in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:28:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:28:51: #1 finished! INFO @ Fri, 10 Dec 2021 09:28:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:28:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:28:54: #2 number of paired peaks: 69 WARNING @ Fri, 10 Dec 2021 09:28:54: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:28:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:28:58: 14000000 INFO @ Fri, 10 Dec 2021 09:29:02: 15000000 INFO @ Fri, 10 Dec 2021 09:29:16: 15000000 INFO @ Fri, 10 Dec 2021 09:29:21: 16000000 INFO @ Fri, 10 Dec 2021 09:29:34: 16000000 INFO @ Fri, 10 Dec 2021 09:29:40: 17000000 INFO @ Fri, 10 Dec 2021 09:29:53: 17000000 INFO @ Fri, 10 Dec 2021 09:29:59: 18000000 INFO @ Fri, 10 Dec 2021 09:30:12: 18000000 INFO @ Fri, 10 Dec 2021 09:30:17: 19000000 INFO @ Fri, 10 Dec 2021 09:30:31: 19000000 INFO @ Fri, 10 Dec 2021 09:30:35: 20000000 INFO @ Fri, 10 Dec 2021 09:30:48: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:30:54: 21000000 INFO @ Fri, 10 Dec 2021 09:31:08: 21000000 INFO @ Fri, 10 Dec 2021 09:31:13: 22000000 INFO @ Fri, 10 Dec 2021 09:31:26: 22000000 INFO @ Fri, 10 Dec 2021 09:31:32: 23000000 INFO @ Fri, 10 Dec 2021 09:31:41: 23000000 INFO @ Fri, 10 Dec 2021 09:31:49: 24000000 INFO @ Fri, 10 Dec 2021 09:31:58: 24000000 INFO @ Fri, 10 Dec 2021 09:32:08: 25000000 INFO @ Fri, 10 Dec 2021 09:32:14: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:32:14: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:32:14: #1 total tags in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:32:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:32:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:32:14: 25000000 INFO @ Fri, 10 Dec 2021 09:32:14: #1 tags after filtering in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:32:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:32:14: #1 finished! INFO @ Fri, 10 Dec 2021 09:32:14: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:32:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:32:17: #2 number of paired peaks: 69 WARNING @ Fri, 10 Dec 2021 09:32:17: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:32:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:32:21: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:32:21: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:32:21: #1 total tags in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:32:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:32:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:32:21: #1 tags after filtering in treatment: 25326804 INFO @ Fri, 10 Dec 2021 09:32:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:32:21: #1 finished! INFO @ Fri, 10 Dec 2021 09:32:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:32:21: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 09:32:24: #2 number of paired peaks: 69 WARNING @ Fri, 10 Dec 2021 09:32:24: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:32:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574260/SRX8574260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling