Job ID = 14170121 SRX = SRX8556378 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 34517969 spots for SRR12024934/SRR12024934.sra Written 34517969 spots for SRR12024934/SRR12024934.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170707 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:46 34517969 reads; of these: 34517969 (100.00%) were paired; of these: 33558494 (97.22%) aligned concordantly 0 times 699288 (2.03%) aligned concordantly exactly 1 time 260187 (0.75%) aligned concordantly >1 times ---- 33558494 pairs aligned concordantly 0 times; of these: 167188 (0.50%) aligned discordantly 1 time ---- 33391306 pairs aligned 0 times concordantly or discordantly; of these: 66782612 mates make up the pairs; of these: 66441085 (99.49%) aligned 0 times 155014 (0.23%) aligned exactly 1 time 186513 (0.28%) aligned >1 times 3.76% overall alignment rate Time searching: 00:13:46 Overall time: 00:13:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 816994 / 1110668 = 0.7356 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:58:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:58:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:58:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 05:58:19: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:58:19: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:58:19: #1 total tags in treatment: 243880 INFO @ Sat, 11 Dec 2021 05:58:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:58:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:58:19: #1 tags after filtering in treatment: 211148 INFO @ Sat, 11 Dec 2021 05:58:19: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:58:19: #1 finished! INFO @ Sat, 11 Dec 2021 05:58:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:58:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:58:19: #2 number of paired peaks: 1884 INFO @ Sat, 11 Dec 2021 05:58:19: start model_add_line... INFO @ Sat, 11 Dec 2021 05:58:19: start X-correlation... INFO @ Sat, 11 Dec 2021 05:58:19: end of X-cor INFO @ Sat, 11 Dec 2021 05:58:19: #2 finished! INFO @ Sat, 11 Dec 2021 05:58:19: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 05:58:19: #2 alternative fragment length(s) may be 168 bps INFO @ Sat, 11 Dec 2021 05:58:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05_model.r WARNING @ Sat, 11 Dec 2021 05:58:19: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:58:19: #2 You may need to consider one of the other alternative d(s): 168 WARNING @ Sat, 11 Dec 2021 05:58:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:58:19: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:58:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:58:19: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:58:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05_peaks.xls INFO @ Sat, 11 Dec 2021 05:58:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:58:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.05_summits.bed INFO @ Sat, 11 Dec 2021 05:58:20: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (474 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:58:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:58:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:58:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 05:58:49: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:58:49: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:58:49: #1 total tags in treatment: 243880 INFO @ Sat, 11 Dec 2021 05:58:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:58:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:58:49: #1 tags after filtering in treatment: 211148 INFO @ Sat, 11 Dec 2021 05:58:49: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:58:49: #1 finished! INFO @ Sat, 11 Dec 2021 05:58:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:58:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:58:49: #2 number of paired peaks: 1884 INFO @ Sat, 11 Dec 2021 05:58:49: start model_add_line... INFO @ Sat, 11 Dec 2021 05:58:49: start X-correlation... INFO @ Sat, 11 Dec 2021 05:58:49: end of X-cor INFO @ Sat, 11 Dec 2021 05:58:49: #2 finished! INFO @ Sat, 11 Dec 2021 05:58:49: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 05:58:49: #2 alternative fragment length(s) may be 168 bps INFO @ Sat, 11 Dec 2021 05:58:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10_model.r WARNING @ Sat, 11 Dec 2021 05:58:49: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:58:49: #2 You may need to consider one of the other alternative d(s): 168 WARNING @ Sat, 11 Dec 2021 05:58:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:58:49: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:58:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:58:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:58:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10_peaks.xls INFO @ Sat, 11 Dec 2021 05:58:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:58:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.10_summits.bed INFO @ Sat, 11 Dec 2021 05:58:50: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (285 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:59:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:59:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:59:12: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 05:59:21: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:59:21: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:59:21: #1 total tags in treatment: 243880 INFO @ Sat, 11 Dec 2021 05:59:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:59:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:59:21: #1 tags after filtering in treatment: 211148 INFO @ Sat, 11 Dec 2021 05:59:21: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:59:21: #1 finished! INFO @ Sat, 11 Dec 2021 05:59:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:59:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:59:21: #2 number of paired peaks: 1884 INFO @ Sat, 11 Dec 2021 05:59:21: start model_add_line... INFO @ Sat, 11 Dec 2021 05:59:21: start X-correlation... INFO @ Sat, 11 Dec 2021 05:59:21: end of X-cor INFO @ Sat, 11 Dec 2021 05:59:21: #2 finished! INFO @ Sat, 11 Dec 2021 05:59:21: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 05:59:21: #2 alternative fragment length(s) may be 168 bps INFO @ Sat, 11 Dec 2021 05:59:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20_model.r WARNING @ Sat, 11 Dec 2021 05:59:21: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:59:21: #2 You may need to consider one of the other alternative d(s): 168 WARNING @ Sat, 11 Dec 2021 05:59:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:59:21: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:59:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:59:21: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:59:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20_peaks.xls INFO @ Sat, 11 Dec 2021 05:59:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:59:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556378/SRX8556378.20_summits.bed INFO @ Sat, 11 Dec 2021 05:59:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (169 records, 4 fields): 12 millis CompletedMACS2peakCalling