Job ID = 14170115 SRX = SRX8556372 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28958643 spots for SRR12024928/SRR12024928.sra Written 28958643 spots for SRR12024928/SRR12024928.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170686 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:00 28958643 reads; of these: 28958643 (100.00%) were paired; of these: 28605035 (98.78%) aligned concordantly 0 times 235964 (0.81%) aligned concordantly exactly 1 time 117644 (0.41%) aligned concordantly >1 times ---- 28605035 pairs aligned concordantly 0 times; of these: 69506 (0.24%) aligned discordantly 1 time ---- 28535529 pairs aligned 0 times concordantly or discordantly; of these: 57071058 mates make up the pairs; of these: 56874040 (99.65%) aligned 0 times 74962 (0.13%) aligned exactly 1 time 122056 (0.21%) aligned >1 times 1.80% overall alignment rate Time searching: 00:09:00 Overall time: 00:09:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 267785 / 414794 = 0.6456 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:40:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:40:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:40:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 05:40:25: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:40:25: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:40:25: #1 total tags in treatment: 123302 INFO @ Sat, 11 Dec 2021 05:40:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:40:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:40:25: #1 tags after filtering in treatment: 107585 INFO @ Sat, 11 Dec 2021 05:40:25: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:40:25: #1 finished! INFO @ Sat, 11 Dec 2021 05:40:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:40:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:40:25: #2 number of paired peaks: 4879 INFO @ Sat, 11 Dec 2021 05:40:25: start model_add_line... INFO @ Sat, 11 Dec 2021 05:40:25: start X-correlation... INFO @ Sat, 11 Dec 2021 05:40:25: end of X-cor INFO @ Sat, 11 Dec 2021 05:40:25: #2 finished! INFO @ Sat, 11 Dec 2021 05:40:25: #2 predicted fragment length is 163 bps INFO @ Sat, 11 Dec 2021 05:40:25: #2 alternative fragment length(s) may be 163 bps INFO @ Sat, 11 Dec 2021 05:40:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05_model.r WARNING @ Sat, 11 Dec 2021 05:40:25: #2 Since the d (163) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:40:25: #2 You may need to consider one of the other alternative d(s): 163 WARNING @ Sat, 11 Dec 2021 05:40:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:40:25: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:40:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:40:25: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:40:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05_peaks.xls INFO @ Sat, 11 Dec 2021 05:40:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:40:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.05_summits.bed INFO @ Sat, 11 Dec 2021 05:40:26: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (413 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:40:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:40:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:40:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 05:40:55: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:40:55: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:40:55: #1 total tags in treatment: 123302 INFO @ Sat, 11 Dec 2021 05:40:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:40:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:40:55: #1 tags after filtering in treatment: 107585 INFO @ Sat, 11 Dec 2021 05:40:55: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:40:55: #1 finished! INFO @ Sat, 11 Dec 2021 05:40:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:40:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:40:55: #2 number of paired peaks: 4879 INFO @ Sat, 11 Dec 2021 05:40:55: start model_add_line... INFO @ Sat, 11 Dec 2021 05:40:55: start X-correlation... INFO @ Sat, 11 Dec 2021 05:40:55: end of X-cor INFO @ Sat, 11 Dec 2021 05:40:55: #2 finished! INFO @ Sat, 11 Dec 2021 05:40:55: #2 predicted fragment length is 163 bps INFO @ Sat, 11 Dec 2021 05:40:55: #2 alternative fragment length(s) may be 163 bps INFO @ Sat, 11 Dec 2021 05:40:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10_model.r WARNING @ Sat, 11 Dec 2021 05:40:55: #2 Since the d (163) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:40:55: #2 You may need to consider one of the other alternative d(s): 163 WARNING @ Sat, 11 Dec 2021 05:40:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:40:55: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:40:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:40:55: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:40:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10_peaks.xls INFO @ Sat, 11 Dec 2021 05:40:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:40:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.10_summits.bed INFO @ Sat, 11 Dec 2021 05:40:55: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (234 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 05:41:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 05:41:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 05:41:20: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 05:41:26: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 05:41:26: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 05:41:26: #1 total tags in treatment: 123302 INFO @ Sat, 11 Dec 2021 05:41:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 05:41:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 05:41:26: #1 tags after filtering in treatment: 107585 INFO @ Sat, 11 Dec 2021 05:41:26: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 05:41:26: #1 finished! INFO @ Sat, 11 Dec 2021 05:41:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 05:41:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 05:41:26: #2 number of paired peaks: 4879 INFO @ Sat, 11 Dec 2021 05:41:26: start model_add_line... INFO @ Sat, 11 Dec 2021 05:41:26: start X-correlation... INFO @ Sat, 11 Dec 2021 05:41:26: end of X-cor INFO @ Sat, 11 Dec 2021 05:41:26: #2 finished! INFO @ Sat, 11 Dec 2021 05:41:26: #2 predicted fragment length is 163 bps INFO @ Sat, 11 Dec 2021 05:41:26: #2 alternative fragment length(s) may be 163 bps INFO @ Sat, 11 Dec 2021 05:41:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20_model.r WARNING @ Sat, 11 Dec 2021 05:41:26: #2 Since the d (163) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 05:41:26: #2 You may need to consider one of the other alternative d(s): 163 WARNING @ Sat, 11 Dec 2021 05:41:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 05:41:26: #3 Call peaks... INFO @ Sat, 11 Dec 2021 05:41:26: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 05:41:27: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 05:41:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20_peaks.xls INFO @ Sat, 11 Dec 2021 05:41:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 05:41:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556372/SRX8556372.20_summits.bed INFO @ Sat, 11 Dec 2021 05:41:27: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (118 records, 4 fields): 1 millis CompletedMACS2peakCalling