Job ID = 14170031 SRX = SRX8556359 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29043947 spots for SRR12024969/SRR12024969.sra Written 29043947 spots for SRR12024969/SRR12024969.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170741 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:28:52 29043947 reads; of these: 29043947 (100.00%) were paired; of these: 8753298 (30.14%) aligned concordantly 0 times 13760448 (47.38%) aligned concordantly exactly 1 time 6530201 (22.48%) aligned concordantly >1 times ---- 8753298 pairs aligned concordantly 0 times; of these: 3169411 (36.21%) aligned discordantly 1 time ---- 5583887 pairs aligned 0 times concordantly or discordantly; of these: 11167774 mates make up the pairs; of these: 7845057 (70.25%) aligned 0 times 649340 (5.81%) aligned exactly 1 time 2673377 (23.94%) aligned >1 times 86.49% overall alignment rate Time searching: 01:28:52 Overall time: 01:28:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2827919 / 23218983 = 0.1218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:40:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:40:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:40:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:40:45: 1000000 INFO @ Sat, 11 Dec 2021 06:40:55: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:41:05: 3000000 INFO @ Sat, 11 Dec 2021 06:41:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:41:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:41:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:41:16: 1000000 INFO @ Sat, 11 Dec 2021 06:41:16: 4000000 INFO @ Sat, 11 Dec 2021 06:41:27: 2000000 INFO @ Sat, 11 Dec 2021 06:41:27: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:41:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:41:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:41:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:41:38: 6000000 INFO @ Sat, 11 Dec 2021 06:41:38: 3000000 INFO @ Sat, 11 Dec 2021 06:41:46: 1000000 INFO @ Sat, 11 Dec 2021 06:41:49: 7000000 INFO @ Sat, 11 Dec 2021 06:41:49: 4000000 INFO @ Sat, 11 Dec 2021 06:41:58: 2000000 INFO @ Sat, 11 Dec 2021 06:42:00: 8000000 INFO @ Sat, 11 Dec 2021 06:42:00: 5000000 INFO @ Sat, 11 Dec 2021 06:42:09: 3000000 INFO @ Sat, 11 Dec 2021 06:42:11: 9000000 INFO @ Sat, 11 Dec 2021 06:42:11: 6000000 INFO @ Sat, 11 Dec 2021 06:42:20: 4000000 INFO @ Sat, 11 Dec 2021 06:42:22: 10000000 INFO @ Sat, 11 Dec 2021 06:42:22: 7000000 INFO @ Sat, 11 Dec 2021 06:42:31: 5000000 INFO @ Sat, 11 Dec 2021 06:42:33: 11000000 INFO @ Sat, 11 Dec 2021 06:42:34: 8000000 INFO @ Sat, 11 Dec 2021 06:42:42: 6000000 INFO @ Sat, 11 Dec 2021 06:42:43: 12000000 INFO @ Sat, 11 Dec 2021 06:42:45: 9000000 INFO @ Sat, 11 Dec 2021 06:42:53: 7000000 INFO @ Sat, 11 Dec 2021 06:42:54: 13000000 INFO @ Sat, 11 Dec 2021 06:42:56: 10000000 INFO @ Sat, 11 Dec 2021 06:43:04: 8000000 INFO @ Sat, 11 Dec 2021 06:43:05: 14000000 INFO @ Sat, 11 Dec 2021 06:43:07: 11000000 INFO @ Sat, 11 Dec 2021 06:43:15: 9000000 INFO @ Sat, 11 Dec 2021 06:43:16: 15000000 INFO @ Sat, 11 Dec 2021 06:43:18: 12000000 INFO @ Sat, 11 Dec 2021 06:43:27: 10000000 INFO @ Sat, 11 Dec 2021 06:43:27: 16000000 INFO @ Sat, 11 Dec 2021 06:43:29: 13000000 INFO @ Sat, 11 Dec 2021 06:43:37: 17000000 INFO @ Sat, 11 Dec 2021 06:43:38: 11000000 INFO @ Sat, 11 Dec 2021 06:43:40: 14000000 INFO @ Sat, 11 Dec 2021 06:43:48: 18000000 INFO @ Sat, 11 Dec 2021 06:43:49: 12000000 INFO @ Sat, 11 Dec 2021 06:43:52: 15000000 INFO @ Sat, 11 Dec 2021 06:43:59: 19000000 INFO @ Sat, 11 Dec 2021 06:44:00: 13000000 INFO @ Sat, 11 Dec 2021 06:44:03: 16000000 INFO @ Sat, 11 Dec 2021 06:44:10: 20000000 INFO @ Sat, 11 Dec 2021 06:44:11: 14000000 INFO @ Sat, 11 Dec 2021 06:44:14: 17000000 INFO @ Sat, 11 Dec 2021 06:44:21: 21000000 INFO @ Sat, 11 Dec 2021 06:44:22: 15000000 INFO @ Sat, 11 Dec 2021 06:44:25: 18000000 INFO @ Sat, 11 Dec 2021 06:44:32: 22000000 INFO @ Sat, 11 Dec 2021 06:44:33: 16000000 INFO @ Sat, 11 Dec 2021 06:44:36: 19000000 INFO @ Sat, 11 Dec 2021 06:44:43: 23000000 INFO @ Sat, 11 Dec 2021 06:44:44: 17000000 INFO @ Sat, 11 Dec 2021 06:44:47: 20000000 INFO @ Sat, 11 Dec 2021 06:44:54: 24000000 INFO @ Sat, 11 Dec 2021 06:44:55: 18000000 INFO @ Sat, 11 Dec 2021 06:44:58: 21000000 INFO @ Sat, 11 Dec 2021 06:45:05: 25000000 INFO @ Sat, 11 Dec 2021 06:45:06: 19000000 INFO @ Sat, 11 Dec 2021 06:45:09: 22000000 INFO @ Sat, 11 Dec 2021 06:45:16: 26000000 INFO @ Sat, 11 Dec 2021 06:45:17: 20000000 INFO @ Sat, 11 Dec 2021 06:45:19: 23000000 INFO @ Sat, 11 Dec 2021 06:45:27: 27000000 INFO @ Sat, 11 Dec 2021 06:45:28: 21000000 INFO @ Sat, 11 Dec 2021 06:45:30: 24000000 INFO @ Sat, 11 Dec 2021 06:45:38: 28000000 INFO @ Sat, 11 Dec 2021 06:45:39: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:45:41: 25000000 INFO @ Sat, 11 Dec 2021 06:45:49: 29000000 INFO @ Sat, 11 Dec 2021 06:45:50: 23000000 INFO @ Sat, 11 Dec 2021 06:45:52: 26000000 INFO @ Sat, 11 Dec 2021 06:45:59: 30000000 INFO @ Sat, 11 Dec 2021 06:46:01: 24000000 INFO @ Sat, 11 Dec 2021 06:46:03: 27000000 INFO @ Sat, 11 Dec 2021 06:46:10: 31000000 INFO @ Sat, 11 Dec 2021 06:46:13: 25000000 INFO @ Sat, 11 Dec 2021 06:46:15: 28000000 INFO @ Sat, 11 Dec 2021 06:46:21: 32000000 INFO @ Sat, 11 Dec 2021 06:46:24: 26000000 INFO @ Sat, 11 Dec 2021 06:46:26: 29000000 INFO @ Sat, 11 Dec 2021 06:46:32: 33000000 INFO @ Sat, 11 Dec 2021 06:46:35: 27000000 INFO @ Sat, 11 Dec 2021 06:46:37: 30000000 INFO @ Sat, 11 Dec 2021 06:46:44: 34000000 INFO @ Sat, 11 Dec 2021 06:46:47: 28000000 INFO @ Sat, 11 Dec 2021 06:46:48: 31000000 INFO @ Sat, 11 Dec 2021 06:46:55: 35000000 INFO @ Sat, 11 Dec 2021 06:46:58: 29000000 INFO @ Sat, 11 Dec 2021 06:46:59: 32000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:47:06: 36000000 INFO @ Sat, 11 Dec 2021 06:47:10: 30000000 INFO @ Sat, 11 Dec 2021 06:47:10: 33000000 INFO @ Sat, 11 Dec 2021 06:47:18: 37000000 INFO @ Sat, 11 Dec 2021 06:47:21: 31000000 INFO @ Sat, 11 Dec 2021 06:47:21: 34000000 INFO @ Sat, 11 Dec 2021 06:47:29: 38000000 INFO @ Sat, 11 Dec 2021 06:47:31: 32000000 INFO @ Sat, 11 Dec 2021 06:47:33: 35000000 INFO @ Sat, 11 Dec 2021 06:47:40: 39000000 INFO @ Sat, 11 Dec 2021 06:47:42: 33000000 INFO @ Sat, 11 Dec 2021 06:47:44: 36000000 INFO @ Sat, 11 Dec 2021 06:47:52: 40000000 INFO @ Sat, 11 Dec 2021 06:47:53: 34000000 INFO @ Sat, 11 Dec 2021 06:47:55: 37000000 INFO @ Sat, 11 Dec 2021 06:48:03: 41000000 INFO @ Sat, 11 Dec 2021 06:48:03: 35000000 INFO @ Sat, 11 Dec 2021 06:48:06: 38000000 INFO @ Sat, 11 Dec 2021 06:48:14: 42000000 INFO @ Sat, 11 Dec 2021 06:48:14: 36000000 INFO @ Sat, 11 Dec 2021 06:48:17: 39000000 INFO @ Sat, 11 Dec 2021 06:48:25: 37000000 INFO @ Sat, 11 Dec 2021 06:48:25: 43000000 INFO @ Sat, 11 Dec 2021 06:48:28: 40000000 INFO @ Sat, 11 Dec 2021 06:48:36: 38000000 INFO @ Sat, 11 Dec 2021 06:48:36: 44000000 INFO @ Sat, 11 Dec 2021 06:48:39: 41000000 INFO @ Sat, 11 Dec 2021 06:48:43: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:48:43: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:48:43: #1 total tags in treatment: 17733696 INFO @ Sat, 11 Dec 2021 06:48:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:48:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:48:43: #1 tags after filtering in treatment: 16060578 INFO @ Sat, 11 Dec 2021 06:48:43: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 06:48:43: #1 finished! INFO @ Sat, 11 Dec 2021 06:48:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:48:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:48:44: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 06:48:44: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:48:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:48:47: 39000000 INFO @ Sat, 11 Dec 2021 06:48:51: 42000000 INFO @ Sat, 11 Dec 2021 06:48:58: 40000000 INFO @ Sat, 11 Dec 2021 06:49:02: 43000000 INFO @ Sat, 11 Dec 2021 06:49:08: 41000000 INFO @ Sat, 11 Dec 2021 06:49:13: 44000000 INFO @ Sat, 11 Dec 2021 06:49:19: 42000000 INFO @ Sat, 11 Dec 2021 06:49:19: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:49:19: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:49:19: #1 total tags in treatment: 17733696 INFO @ Sat, 11 Dec 2021 06:49:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:49:20: #1 tags after filtering in treatment: 16060578 INFO @ Sat, 11 Dec 2021 06:49:20: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 06:49:20: #1 finished! INFO @ Sat, 11 Dec 2021 06:49:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:49:21: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 06:49:21: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:49:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:49:29: 43000000 INFO @ Sat, 11 Dec 2021 06:49:39: 44000000 INFO @ Sat, 11 Dec 2021 06:49:45: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:49:45: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:49:45: #1 total tags in treatment: 17733696 INFO @ Sat, 11 Dec 2021 06:49:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:49:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:49:45: #1 tags after filtering in treatment: 16060578 INFO @ Sat, 11 Dec 2021 06:49:45: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 06:49:45: #1 finished! INFO @ Sat, 11 Dec 2021 06:49:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:49:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:49:46: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 06:49:46: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:49:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556359/SRX8556359.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling