Job ID = 6627276 SRX = SRX8521343 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:47:41 prefetch.2.10.7: 1) Downloading 'SRR11978584'... 2020-07-14T01:47:41 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:56 prefetch.2.10.7: 'SRR11978584' is valid 2020-07-14T01:47:56 prefetch.2.10.7: 1) 'SRR11978584' was downloaded successfully 2020-07-14T01:47:56 prefetch.2.10.7: 'SRR11978584' has 0 unresolved dependencies Read 278982 spots for SRR11978584/SRR11978584.sra Written 278982 spots for SRR11978584/SRR11978584.sra 2020-07-14T01:48:11 prefetch.2.10.7: 1) Downloading 'SRR11978585'... 2020-07-14T01:48:11 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:39 prefetch.2.10.7: 'SRR11978585' is valid 2020-07-14T01:48:39 prefetch.2.10.7: 1) 'SRR11978585' was downloaded successfully 2020-07-14T01:48:39 prefetch.2.10.7: 'SRR11978585' has 0 unresolved dependencies Read 1003563 spots for SRR11978585/SRR11978585.sra Written 1003563 spots for SRR11978585/SRR11978585.sra 2020-07-14T01:48:53 prefetch.2.10.7: 1) Downloading 'SRR11978586'... 2020-07-14T01:48:53 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:15 prefetch.2.10.7: 'SRR11978586' is valid 2020-07-14T01:49:15 prefetch.2.10.7: 1) 'SRR11978586' was downloaded successfully 2020-07-14T01:49:15 prefetch.2.10.7: 'SRR11978586' has 0 unresolved dependencies Read 1067910 spots for SRR11978586/SRR11978586.sra Written 1067910 spots for SRR11978586/SRR11978586.sra 2020-07-14T01:49:31 prefetch.2.10.7: 1) Downloading 'SRR11978587'... 2020-07-14T01:49:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:51 prefetch.2.10.7: 'SRR11978587' is valid 2020-07-14T01:49:51 prefetch.2.10.7: 1) 'SRR11978587' was downloaded successfully 2020-07-14T01:49:51 prefetch.2.10.7: 'SRR11978587' has 0 unresolved dependencies Read 1043636 spots for SRR11978587/SRR11978587.sra Written 1043636 spots for SRR11978587/SRR11978587.sra 2020-07-14T01:50:05 prefetch.2.10.7: 1) Downloading 'SRR11978588'... 2020-07-14T01:50:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:50:33 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:50:33 prefetch.2.10.7: 'SRR11978588' is valid 2020-07-14T01:50:33 prefetch.2.10.7: 1) 'SRR11978588' was downloaded successfully 2020-07-14T01:50:33 prefetch.2.10.7: 'SRR11978588' has 0 unresolved dependencies Read 2015949 spots for SRR11978588/SRR11978588.sra Written 2015949 spots for SRR11978588/SRR11978588.sra 2020-07-14T01:50:51 prefetch.2.10.7: 1) Downloading 'SRR11978589'... 2020-07-14T01:50:51 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:16 prefetch.2.10.7: 'SRR11978589' is valid 2020-07-14T01:51:16 prefetch.2.10.7: 1) 'SRR11978589' was downloaded successfully 2020-07-14T01:51:16 prefetch.2.10.7: 'SRR11978589' has 0 unresolved dependencies Read 2046744 spots for SRR11978589/SRR11978589.sra Written 2046744 spots for SRR11978589/SRR11978589.sra 2020-07-14T01:51:33 prefetch.2.10.7: 1) Downloading 'SRR11978590'... 2020-07-14T01:51:33 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:56 prefetch.2.10.7: 'SRR11978590' is valid 2020-07-14T01:51:56 prefetch.2.10.7: 1) 'SRR11978590' was downloaded successfully 2020-07-14T01:51:56 prefetch.2.10.7: 'SRR11978590' has 0 unresolved dependencies Read 2050595 spots for SRR11978590/SRR11978590.sra Written 2050595 spots for SRR11978590/SRR11978590.sra 2020-07-14T01:52:17 prefetch.2.10.7: 1) Downloading 'SRR11978591'... 2020-07-14T01:52:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:52:36 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:52:36 prefetch.2.10.7: 'SRR11978591' is valid 2020-07-14T01:52:36 prefetch.2.10.7: 1) 'SRR11978591' was downloaded successfully 2020-07-14T01:52:36 prefetch.2.10.7: 'SRR11978591' has 0 unresolved dependencies Read 2017854 spots for SRR11978591/SRR11978591.sra Written 2017854 spots for SRR11978591/SRR11978591.sra 2020-07-14T01:53:00 prefetch.2.10.7: 1) Downloading 'SRR11978592'... 2020-07-14T01:53:00 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:53:18 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:53:18 prefetch.2.10.7: 'SRR11978592' is valid 2020-07-14T01:53:18 prefetch.2.10.7: 1) 'SRR11978592' was downloaded successfully 2020-07-14T01:53:18 prefetch.2.10.7: 'SRR11978592' has 0 unresolved dependencies Read 268807 spots for SRR11978592/SRR11978592.sra Written 268807 spots for SRR11978592/SRR11978592.sra 2020-07-14T01:53:34 prefetch.2.10.7: 1) Downloading 'SRR11978593'... 2020-07-14T01:53:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:53:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:53:55 prefetch.2.10.7: 'SRR11978593' is valid 2020-07-14T01:53:55 prefetch.2.10.7: 1) 'SRR11978593' was downloaded successfully 2020-07-14T01:53:55 prefetch.2.10.7: 'SRR11978593' has 0 unresolved dependencies Read 284179 spots for SRR11978593/SRR11978593.sra Written 284179 spots for SRR11978593/SRR11978593.sra 2020-07-14T01:54:06 prefetch.2.10.7: 1) Downloading 'SRR11978594'... 2020-07-14T01:54:06 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:54:20 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:54:20 prefetch.2.10.7: 'SRR11978594' is valid 2020-07-14T01:54:20 prefetch.2.10.7: 1) 'SRR11978594' was downloaded successfully 2020-07-14T01:54:20 prefetch.2.10.7: 'SRR11978594' has 0 unresolved dependencies Read 280758 spots for SRR11978594/SRR11978594.sra Written 280758 spots for SRR11978594/SRR11978594.sra 2020-07-14T01:54:34 prefetch.2.10.7: 1) Downloading 'SRR11978595'... 2020-07-14T01:54:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:36 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:37 prefetch.2.10.7: 'SRR11978595' is valid 2020-07-14T01:56:37 prefetch.2.10.7: 1) 'SRR11978595' was downloaded successfully 2020-07-14T01:56:37 prefetch.2.10.7: 'SRR11978595' has 0 unresolved dependencies Read 7729844 spots for SRR11978595/SRR11978595.sra Written 7729844 spots for SRR11978595/SRR11978595.sra 2020-07-14T01:57:15 prefetch.2.10.7: 1) Downloading 'SRR11978596'... 2020-07-14T01:57:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:58:47 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:58:47 prefetch.2.10.7: 'SRR11978596' is valid 2020-07-14T01:58:47 prefetch.2.10.7: 1) 'SRR11978596' was downloaded successfully 2020-07-14T01:58:47 prefetch.2.10.7: 'SRR11978596' has 0 unresolved dependencies Read 7553895 spots for SRR11978596/SRR11978596.sra Written 7553895 spots for SRR11978596/SRR11978596.sra 2020-07-14T01:59:26 prefetch.2.10.7: 1) Downloading 'SRR11978597'... 2020-07-14T01:59:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:02:21 prefetch.2.10.7: HTTPS download failed 2020-07-14T02:02:21 prefetch.2.10.7: 1) failed to download SRR11978597 2020-07-14T02:02:37 prefetch.2.10.7: 1) Downloading 'SRR11978597'... 2020-07-14T02:02:37 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:02:37 prefetch.2.10.7: Continue download of 'SRR11978597' from 222698687 2020-07-14T02:02:46 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:02:46 prefetch.2.10.7: 'SRR11978597' is valid 2020-07-14T02:02:46 prefetch.2.10.7: 1) 'SRR11978597' was downloaded successfully 2020-07-14T02:02:46 prefetch.2.10.7: 'SRR11978597' has 0 unresolved dependencies Read 7828429 spots for SRR11978597/SRR11978597.sra Written 7828429 spots for SRR11978597/SRR11978597.sra 2020-07-14T02:03:23 prefetch.2.10.7: 1) Downloading 'SRR11978598'... 2020-07-14T02:03:23 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:04:17 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:04:17 prefetch.2.10.7: 'SRR11978598' is valid 2020-07-14T02:04:17 prefetch.2.10.7: 1) 'SRR11978598' was downloaded successfully 2020-07-14T02:04:17 prefetch.2.10.7: 'SRR11978598' has 0 unresolved dependencies Read 7829531 spots for SRR11978598/SRR11978598.sra Written 7829531 spots for SRR11978598/SRR11978598.sra 2020-07-14T02:04:54 prefetch.2.10.7: 1) Downloading 'SRR11978599'... 2020-07-14T02:04:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:05:19 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:05:19 prefetch.2.10.7: 'SRR11978599' is valid 2020-07-14T02:05:19 prefetch.2.10.7: 1) 'SRR11978599' was downloaded successfully 2020-07-14T02:05:19 prefetch.2.10.7: 'SRR11978599' has 0 unresolved dependencies Read 1040895 spots for SRR11978599/SRR11978599.sra Written 1040895 spots for SRR11978599/SRR11978599.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627529 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 44341571 reads; of these: 44341571 (100.00%) were unpaired; of these: 38373796 (86.54%) aligned 0 times 4641739 (10.47%) aligned exactly 1 time 1326036 (2.99%) aligned >1 times 13.46% overall alignment rate Time searching: 00:06:48 Overall time: 00:06:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1504658 / 5967775 = 0.2521 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:14:34: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:14:34: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:14:39: 1000000 INFO @ Tue, 14 Jul 2020 11:14:44: 2000000 INFO @ Tue, 14 Jul 2020 11:14:49: 3000000 INFO @ Tue, 14 Jul 2020 11:14:54: 4000000 INFO @ Tue, 14 Jul 2020 11:14:57: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:14:57: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:14:57: #1 total tags in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:14:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:14:57: #1 tags after filtering in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:14:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:14:57: #1 finished! INFO @ Tue, 14 Jul 2020 11:14:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:14:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:14:57: #2 number of paired peaks: 554 WARNING @ Tue, 14 Jul 2020 11:14:57: Fewer paired peaks (554) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 554 pairs to build model! INFO @ Tue, 14 Jul 2020 11:14:57: start model_add_line... INFO @ Tue, 14 Jul 2020 11:14:57: start X-correlation... INFO @ Tue, 14 Jul 2020 11:14:57: end of X-cor INFO @ Tue, 14 Jul 2020 11:14:57: #2 finished! INFO @ Tue, 14 Jul 2020 11:14:57: #2 predicted fragment length is 66 bps INFO @ Tue, 14 Jul 2020 11:14:57: #2 alternative fragment length(s) may be 66 bps INFO @ Tue, 14 Jul 2020 11:14:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05_model.r WARNING @ Tue, 14 Jul 2020 11:14:57: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:14:57: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Tue, 14 Jul 2020 11:14:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:14:57: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:14:57: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:15:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:15:04: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:15:04: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:15:07: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:15:09: 1000000 INFO @ Tue, 14 Jul 2020 11:15:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:15:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:15:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.05_summits.bed INFO @ Tue, 14 Jul 2020 11:15:11: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1852 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:15:14: 2000000 INFO @ Tue, 14 Jul 2020 11:15:19: 3000000 INFO @ Tue, 14 Jul 2020 11:15:24: 4000000 INFO @ Tue, 14 Jul 2020 11:15:27: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:15:27: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:15:27: #1 total tags in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:15:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:15:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:15:27: #1 tags after filtering in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:15:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:15:27: #1 finished! INFO @ Tue, 14 Jul 2020 11:15:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:15:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:15:27: #2 number of paired peaks: 554 WARNING @ Tue, 14 Jul 2020 11:15:27: Fewer paired peaks (554) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 554 pairs to build model! INFO @ Tue, 14 Jul 2020 11:15:27: start model_add_line... INFO @ Tue, 14 Jul 2020 11:15:27: start X-correlation... INFO @ Tue, 14 Jul 2020 11:15:27: end of X-cor INFO @ Tue, 14 Jul 2020 11:15:27: #2 finished! INFO @ Tue, 14 Jul 2020 11:15:27: #2 predicted fragment length is 66 bps INFO @ Tue, 14 Jul 2020 11:15:27: #2 alternative fragment length(s) may be 66 bps INFO @ Tue, 14 Jul 2020 11:15:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10_model.r WARNING @ Tue, 14 Jul 2020 11:15:27: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:15:27: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Tue, 14 Jul 2020 11:15:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:15:27: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:15:27: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:15:34: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:15:34: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:15:37: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:15:40: 1000000 INFO @ Tue, 14 Jul 2020 11:15:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:15:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:15:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.10_summits.bed INFO @ Tue, 14 Jul 2020 11:15:41: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (665 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:15:45: 2000000 INFO @ Tue, 14 Jul 2020 11:15:51: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:15:56: 4000000 INFO @ Tue, 14 Jul 2020 11:15:59: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:15:59: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:15:59: #1 total tags in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:15:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:15:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:15:59: #1 tags after filtering in treatment: 4463117 INFO @ Tue, 14 Jul 2020 11:15:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:15:59: #1 finished! INFO @ Tue, 14 Jul 2020 11:15:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:15:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:16:00: #2 number of paired peaks: 554 WARNING @ Tue, 14 Jul 2020 11:16:00: Fewer paired peaks (554) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 554 pairs to build model! INFO @ Tue, 14 Jul 2020 11:16:00: start model_add_line... INFO @ Tue, 14 Jul 2020 11:16:00: start X-correlation... INFO @ Tue, 14 Jul 2020 11:16:00: end of X-cor INFO @ Tue, 14 Jul 2020 11:16:00: #2 finished! INFO @ Tue, 14 Jul 2020 11:16:00: #2 predicted fragment length is 66 bps INFO @ Tue, 14 Jul 2020 11:16:00: #2 alternative fragment length(s) may be 66 bps INFO @ Tue, 14 Jul 2020 11:16:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20_model.r WARNING @ Tue, 14 Jul 2020 11:16:00: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:16:00: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Tue, 14 Jul 2020 11:16:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:16:00: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:16:00: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:16:09: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:16:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:16:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:16:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521343/SRX8521343.20_summits.bed INFO @ Tue, 14 Jul 2020 11:16:14: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (175 records, 4 fields): 19 millis CompletedMACS2peakCalling