Job ID = 6627268 SRX = SRX8521336 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:44:30 prefetch.2.10.7: 1) Downloading 'SRR11978045'... 2020-07-14T01:44:30 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:31 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:31 prefetch.2.10.7: 1) 'SRR11978045' was downloaded successfully 2020-07-14T01:49:31 prefetch.2.10.7: 'SRR11978045' has 0 unresolved dependencies Read 21228948 spots for SRR11978045/SRR11978045.sra Written 21228948 spots for SRR11978045/SRR11978045.sra 2020-07-14T01:50:57 prefetch.2.10.7: 1) Downloading 'SRR11978046'... 2020-07-14T01:50:57 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:51 prefetch.2.10.7: 'SRR11978046' is valid 2020-07-14T01:55:51 prefetch.2.10.7: 1) 'SRR11978046' was downloaded successfully 2020-07-14T01:55:51 prefetch.2.10.7: 'SRR11978046' has 0 unresolved dependencies Read 8100254 spots for SRR11978046/SRR11978046.sra Written 8100254 spots for SRR11978046/SRR11978046.sra 2020-07-14T01:56:32 prefetch.2.10.7: 1) Downloading 'SRR11978047'... 2020-07-14T01:56:32 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:57:36 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:57:37 prefetch.2.10.7: 'SRR11978047' is valid 2020-07-14T01:57:37 prefetch.2.10.7: 1) 'SRR11978047' was downloaded successfully 2020-07-14T01:57:37 prefetch.2.10.7: 'SRR11978047' has 0 unresolved dependencies Read 8414013 spots for SRR11978047/SRR11978047.sra Written 8414013 spots for SRR11978047/SRR11978047.sra 2020-07-14T01:58:18 prefetch.2.10.7: 1) Downloading 'SRR11978048'... 2020-07-14T01:58:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:59:25 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:59:25 prefetch.2.10.7: 'SRR11978048' is valid 2020-07-14T01:59:25 prefetch.2.10.7: 1) 'SRR11978048' was downloaded successfully 2020-07-14T01:59:25 prefetch.2.10.7: 'SRR11978048' has 0 unresolved dependencies Read 8290926 spots for SRR11978048/SRR11978048.sra Written 8290926 spots for SRR11978048/SRR11978048.sra 2020-07-14T02:00:19 prefetch.2.10.7: 1) Downloading 'SRR11978049'... 2020-07-14T02:00:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:03:18 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:03:18 prefetch.2.10.7: 1) 'SRR11978049' was downloaded successfully 2020-07-14T02:03:18 prefetch.2.10.7: 'SRR11978049' has 0 unresolved dependencies Read 20614183 spots for SRR11978049/SRR11978049.sra Written 20614183 spots for SRR11978049/SRR11978049.sra 2020-07-14T02:04:43 prefetch.2.10.7: 1) Downloading 'SRR11978537'... 2020-07-14T02:04:43 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:06:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:06:53 prefetch.2.10.7: 1) 'SRR11978537' was downloaded successfully 2020-07-14T02:06:53 prefetch.2.10.7: 'SRR11978537' has 0 unresolved dependencies Read 21509032 spots for SRR11978537/SRR11978537.sra Written 21509032 spots for SRR11978537/SRR11978537.sra 2020-07-14T02:08:15 prefetch.2.10.7: 1) Downloading 'SRR11978538'... 2020-07-14T02:08:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:11:32 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:11:32 prefetch.2.10.7: 1) 'SRR11978538' was downloaded successfully 2020-07-14T02:11:32 prefetch.2.10.7: 'SRR11978538' has 0 unresolved dependencies Read 21548901 spots for SRR11978538/SRR11978538.sra Written 21548901 spots for SRR11978538/SRR11978538.sra 2020-07-14T02:13:03 prefetch.2.10.7: 1) Downloading 'SRR11978539'... 2020-07-14T02:13:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:13:35 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:13:35 prefetch.2.10.7: 'SRR11978539' is valid 2020-07-14T02:13:35 prefetch.2.10.7: 1) 'SRR11978539' was downloaded successfully 2020-07-14T02:13:35 prefetch.2.10.7: 'SRR11978539' has 0 unresolved dependencies Read 1528056 spots for SRR11978539/SRR11978539.sra Written 1528056 spots for SRR11978539/SRR11978539.sra 2020-07-14T02:13:53 prefetch.2.10.7: 1) Downloading 'SRR11978540'... 2020-07-14T02:13:53 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:14:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:14:22 prefetch.2.10.7: 'SRR11978540' is valid 2020-07-14T02:14:22 prefetch.2.10.7: 1) 'SRR11978540' was downloaded successfully 2020-07-14T02:14:22 prefetch.2.10.7: 'SRR11978540' has 0 unresolved dependencies Read 1478674 spots for SRR11978540/SRR11978540.sra Written 1478674 spots for SRR11978540/SRR11978540.sra 2020-07-14T02:14:42 prefetch.2.10.7: 1) Downloading 'SRR11978541'... 2020-07-14T02:14:42 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:15:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:15:10 prefetch.2.10.7: 'SRR11978541' is valid 2020-07-14T02:15:10 prefetch.2.10.7: 1) 'SRR11978541' was downloaded successfully 2020-07-14T02:15:10 prefetch.2.10.7: 'SRR11978541' has 0 unresolved dependencies Read 1566409 spots for SRR11978541/SRR11978541.sra Written 1566409 spots for SRR11978541/SRR11978541.sra 2020-07-14T02:15:29 prefetch.2.10.7: 1) Downloading 'SRR11978542'... 2020-07-14T02:15:30 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:15:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:15:53 prefetch.2.10.7: 'SRR11978542' is valid 2020-07-14T02:15:53 prefetch.2.10.7: 1) 'SRR11978542' was downloaded successfully 2020-07-14T02:15:53 prefetch.2.10.7: 'SRR11978542' has 0 unresolved dependencies Read 1535151 spots for SRR11978542/SRR11978542.sra Written 1535151 spots for SRR11978542/SRR11978542.sra 2020-07-14T02:16:13 prefetch.2.10.7: 1) Downloading 'SRR11978543'... 2020-07-14T02:16:13 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:17:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:17:41 prefetch.2.10.7: 'SRR11978543' is valid 2020-07-14T02:17:41 prefetch.2.10.7: 1) 'SRR11978543' was downloaded successfully 2020-07-14T02:17:41 prefetch.2.10.7: 'SRR11978543' has 0 unresolved dependencies Read 8276821 spots for SRR11978543/SRR11978543.sra Written 8276821 spots for SRR11978543/SRR11978543.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627631 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:11 124091368 reads; of these: 124091368 (100.00%) were unpaired; of these: 100001322 (80.59%) aligned 0 times 14914491 (12.02%) aligned exactly 1 time 9175555 (7.39%) aligned >1 times 19.41% overall alignment rate Time searching: 00:26:12 Overall time: 00:26:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8110921 / 24090046 = 0.3367 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:52:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:52:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:52:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:52:48: 1000000 INFO @ Tue, 14 Jul 2020 11:52:53: 2000000 INFO @ Tue, 14 Jul 2020 11:52:59: 3000000 INFO @ Tue, 14 Jul 2020 11:53:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:53:10: 5000000 INFO @ Tue, 14 Jul 2020 11:53:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:53:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:53:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:53:16: 6000000 INFO @ Tue, 14 Jul 2020 11:53:17: 1000000 INFO @ Tue, 14 Jul 2020 11:53:22: 2000000 INFO @ Tue, 14 Jul 2020 11:53:22: 7000000 INFO @ Tue, 14 Jul 2020 11:53:27: 3000000 INFO @ Tue, 14 Jul 2020 11:53:28: 8000000 INFO @ Tue, 14 Jul 2020 11:53:32: 4000000 INFO @ Tue, 14 Jul 2020 11:53:33: 9000000 INFO @ Tue, 14 Jul 2020 11:53:37: 5000000 INFO @ Tue, 14 Jul 2020 11:53:39: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:53:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:53:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:53:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:53:42: 6000000 INFO @ Tue, 14 Jul 2020 11:53:45: 11000000 INFO @ Tue, 14 Jul 2020 11:53:47: 7000000 INFO @ Tue, 14 Jul 2020 11:53:47: 1000000 INFO @ Tue, 14 Jul 2020 11:53:51: 12000000 INFO @ Tue, 14 Jul 2020 11:53:52: 8000000 INFO @ Tue, 14 Jul 2020 11:53:52: 2000000 INFO @ Tue, 14 Jul 2020 11:53:57: 13000000 INFO @ Tue, 14 Jul 2020 11:53:57: 9000000 INFO @ Tue, 14 Jul 2020 11:53:57: 3000000 INFO @ Tue, 14 Jul 2020 11:54:02: 10000000 INFO @ Tue, 14 Jul 2020 11:54:02: 14000000 INFO @ Tue, 14 Jul 2020 11:54:03: 4000000 INFO @ Tue, 14 Jul 2020 11:54:07: 11000000 INFO @ Tue, 14 Jul 2020 11:54:08: 5000000 INFO @ Tue, 14 Jul 2020 11:54:08: 15000000 INFO @ Tue, 14 Jul 2020 11:54:12: 12000000 INFO @ Tue, 14 Jul 2020 11:54:13: 6000000 INFO @ Tue, 14 Jul 2020 11:54:14: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 11:54:14: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 11:54:14: #1 total tags in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:54:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:54:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:54:14: #1 tags after filtering in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:54:14: #1 finished! INFO @ Tue, 14 Jul 2020 11:54:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:54:15: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 11:54:15: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:54:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:54:17: 13000000 INFO @ Tue, 14 Jul 2020 11:54:18: 7000000 INFO @ Tue, 14 Jul 2020 11:54:22: 14000000 INFO @ Tue, 14 Jul 2020 11:54:23: 8000000 INFO @ Tue, 14 Jul 2020 11:54:27: 15000000 INFO @ Tue, 14 Jul 2020 11:54:28: 9000000 INFO @ Tue, 14 Jul 2020 11:54:32: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 11:54:32: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 11:54:32: #1 total tags in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:54:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:54:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:54:32: #1 tags after filtering in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:54:32: #1 finished! INFO @ Tue, 14 Jul 2020 11:54:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:54:33: 10000000 INFO @ Tue, 14 Jul 2020 11:54:33: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 11:54:33: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:54:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:54:38: 11000000 INFO @ Tue, 14 Jul 2020 11:54:43: 12000000 INFO @ Tue, 14 Jul 2020 11:54:49: 13000000 INFO @ Tue, 14 Jul 2020 11:54:54: 14000000 INFO @ Tue, 14 Jul 2020 11:54:59: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:55:04: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 11:55:04: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 11:55:04: #1 total tags in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:55:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:55:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:55:05: #1 tags after filtering in treatment: 15979125 INFO @ Tue, 14 Jul 2020 11:55:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:55:05: #1 finished! INFO @ Tue, 14 Jul 2020 11:55:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:55:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:55:06: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 11:55:06: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:55:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521336/SRX8521336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。